Management of the soybean cyst nematode Heterodera glycines with combinations of different rhizobacterial strains on soybean

Abstract

Soybean cyst nematode (SCN) is the most damaging soybean pest worldwide. To improve soybean resistance to SCN, we employed a soybean seed-coating strategy through combination of three rhizobacterial strains, including Bacillus simple, B. megaterium and Sinarhizobium fredii at various ratios. We found seed coating by such rhizobacterial strains at a ratio of 3:1:1 (thereafter called SN101) produced the highest germination rate and the mortality of J2 of nematodes. Then, the role of soybean seed coating by SN101 in nematode control was evaluated under both greenhouse and two field conditions in Northeast China in 2013 and 2014. Our results showed that SN101 treatment greatly reduced SCN reproduction and significantly promoted plant growth and yield production in both greenhouse and field trials, suggesting that SN101 is a promising seed-coating agent that may be used as an alternative bio-nematicide for controlling SCN in soybean fields. Our findings also demonstrate that combination of multiple rhizobacterial strains needs to be considered in the seed coating for better management of plant nematodes.

Biocontrol potential ofMicrobacterium maritypicumSneb159 againstHeterodera glycines

“…Morphological, biochemical and molecular tools were used to identify Sneb159 as M. maritypicum . Coating seeds with B. simplex strain Sneb545 or compound bacteria SN101 promoted soybean resistance to H. glycines . For H. glycines , the females are important because they determine the size of a population and the extent of the damage .…”

“…Coating seeds with B. simplex strain Sneb545 or compound bacteria SN101 promoted soybean resistance to H. glycines. 37,46 For H. glycines, the females are important because they determine the size of a population and the extent of the damage. 47 Seeds treated with Sneb159 reduced population of juveniles and females in growth chamber and field experiments.…”

“…Seeds were surface‐sterilized with 0.5% NaClO for 10 min and washed several times with SDW and air‐dried . The sterilized seeds were uniformly coated with bacterial cultures (10 8 CFU/mL) at a seed to fresh culture ratio of 70:1 (g/mL); seeds coated with SDW were used as a control …”

Isolation and identification of induced systemic resistance determinants from Bacillus simplex Sneb545 against Heterodera glycines

“…The exogenous stimulation of PGPR can induce a plant immune response, which can directly induce salicylic acid (SA)-and/or jasmonic acid (JA)-dependent defence responses 11,12 . Previous studies have indicated that the expression of SA-and JA-pathway-related genes could be up-regulated in host plants in response to SCN by coating seeds with bacteria [13][14][15] .…”

Potassium-induced plant resistance against soybean cyst nematode via root exudation of phenolic acids and plant pathogen-related genes

“…Normally, nematicide and soil fumigation are proposed to control SCN [ 5 , 6 ]. However, such treatments seriously affect the ecological environment [ 7 , 8 ]. Therefore, simple tactics with fewer environmental risks have been sought to prevent SCN.…”