This study explores the effect of manufacturing flexibility on the operational performance of Pharmaceutical manufacturing firms in Jordan, variables such as of machine, volume, material handling, mix, and routing flexibilities are selected to represent the manufacturing flexibility dimension while quality, cost, speed and reliability are chosen to represent operational performance.A survey questionnaire was distributed for that purpose to the subjects who belong to different departments or divisions in the production function. The study revealed that manufacturing flexibility is being adopted to a moderate extent, while the operational performance is impressively high. Testing hypothesis indicated that the manufacturing flexibility dimensions have a significant effect on the operational hypothesis performance of the pharmaceutical manufacturing companies, hence, the null hypothesis is rejected and the alternative hypothesis is accepted.