Manganese porphyrins — Studies on their potential use for protein labeling

Konopińska, Kamila; Pietrzak, Mariusz; Malinowska, Elżbieta

doi:10.1016/j.microc.2014.01.013

Cited by 9 publications

(7 citation statements)

References 53 publications

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“…The same results were obtained for BSA and IgG. Comparing porphyrins with tin and manganese cations in the coordination center, we observed the same correlation in the Soret band intensities; however, shifts toward lower frequencies were more apparent in the case of Sn-tpp [36]. The detection limit for Sn-tpp obtained by UV-Vis spectrophotometry was 2.5 nM with a linear range of response up to 5 lM (in the case of Mn-tpp: 0.5 nM with a linear range of response up to 20 lM).…”

Section: Optical Characterizationsupporting

confidence: 84%

“…For each of the four examined electrolytes, a decrease of the emission intensity was found, with the largest decline in the case of bromide. Second, we found that the addition of proteins caused only slight negligible changes of signal intensities, as was also reported for manganese porphyrin [36]. The fluorescence spectra for Sn-tpp at different concentrations of OVA are presented in Fig.…”

Section: Optical Characterizationsupporting

confidence: 82%

“…It is shown that we can control the proteins' impact on Sn-tpp signals, which was a necessary step before we proceeded to the conjugation with proteins. The obtained results were compared with a similar study conducted for 5,10,15,20-tetraphenylporphyrin manganese(III) chloride (Mn-tpp), which is also a compound predestinated for the role of hybrid biomolecules' label [36].…”

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confidence: 97%

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Studies on potential use of tin(IV) porphyrin in a role of proteins’ label

Konopińska

Pietrzak

Malinowska

2015

Analytical Biochemistry

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Section: Optical Characterizationsupporting

confidence: 84%

Section: Optical Characterizationsupporting

confidence: 82%

mentioning

confidence: 97%

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Studies on potential use of tin(IV) porphyrin in a role of proteins’ label

Konopińska

Pietrzak

Malinowska

2015

Analytical Biochemistry

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“…Synthetic manganese porphyrins have been widely studied, with respect to their potential applications in the fields of catalysis and functional materials, − and several meso - and/or β-substituted manganese porphyrins have also been electrochemically examined. − However, to the best of our knowledge, no electrochemical data on manganese(III) β,β′-butanoporphyrins has been reported in the literature and only a few manganese(III) β,β′-benzo-substituted tetraarylporphyrins have been characterized to date. − These earlier studies on benzoporphyrins were devoted mainly to oxidations and applications of the compounds in catalysis, and very little is known about their reductive behavior, which we wished to investigate in detail.…”

Section: Introductionmentioning

confidence: 99%

Spectral, Electrochemical, and ESR Characterization of Manganese Tetraarylporphyrins Containing Four β,β′-Pyrrole Fused Butano and Benzo Groups in Nonaqueous Media

Fang

Wang

et al. 2019

Inorg. Chem.

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Two series of β,β′-pyrrole butano- and benzo-substituted mangenese(III) tetraarylporphyrins were synthesized and characterized with regard to their spectral and electrochemical properties. The investigated compounds have the general formula butano(Ar)4PorMnCl and benzo(Ar)4PorMnCl, where Por is the dianion of the porphyrin and Ar is a p-CH3Ph, Ph or p-ClPh group on each of the four meso-positions of the macrocycle. Each manganese(III) butano- or benzoporphyrin was examined in CH2Cl2 and/or pyridine containing 0.1 M tetra-n-butylammonium perchlorate and the data then were compared to that of the parent tetraarylporphyrins having the same meso-substituents. Up to four reductions are observed for each compound, the first being metal-centered to generate a Mn(II) porphyrin, and the second and third being porphyrin ring-centered to give a Mn(II) porphyrin π-anion radical and dianion, respectively. The one-electron reduced manganese porphyrins have an ESR spectrum with signals at g ⊥= 5.6–5.8 and g // = 2.0, indicating a mixture of the four- and five-coordinated Mn(II) complexes in a high-spin state (3d5, S = 5/2, I = 5/2). Data from cyclic voltammetry and spectroelectrochemistry both suggest that formation of the porphyrin dianion is followed by a chemical reaction at the electrode surface to give an electroactive phlorin anion. The effects of solvent and porphyrin substituents on ultraviolet–visible light (UV-vis) spectra, redox potentials, and electron transfer mechanisms are discussed.

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“…Finally there exists a possibility of applying the optimized conjugation procedure established in this work to bind proteins with metalated Tpp derivatives containing manganese(III) (Mn-tpp) and tin(IV) (Sn-tpp) cations in the coordination center. The characteristics of both Mn-tpp and Sn-tpp and the possibilities of their determination were presented elsewhere [ 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

Tetraphenylporphyrin as a protein label for triple detection analytical systems

Konopińska

Pietrzak

Mazur

et al. 2015

Heliyon

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Porphyrins and metalloporphyrins are promising new protein labels that can be detected using multiple techniques; improving the reliability of the analysis and broadening the range of the linear response. Here, we investigate the potential of 5,10,15,20-tetraphenyl-21H,23H-porphyrin (Tpp) as a hybrid protein label. The electrochemical and optical properties of porphyrin conjugated with bovine serum albumin (BSA), chicken egg albumin (CEA) and immunoglobulin G (IgG) were determined and optimal conditions for Tpp-protein conjugation established. Model conjugates of carboxylated Tpp with BSA and short peptides were characterized using differential pulse voltammetry, UV–Vis spectrophotometry and spectrofluorimetry. These results reveal that Tpp is a promising molecule to be used in a triple detection protein labelling system.

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Manganese porphyrins — Studies on their potential use for protein labeling

Cited by 9 publications

References 53 publications

Studies on potential use of tin(IV) porphyrin in a role of proteins’ label

Studies on potential use of tin(IV) porphyrin in a role of proteins’ label

Spectral, Electrochemical, and ESR Characterization of Manganese Tetraarylporphyrins Containing Four β,β′-Pyrrole Fused Butano and Benzo Groups in Nonaqueous Media

Tetraphenylporphyrin as a protein label for triple detection analytical systems

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