Manganese superoxide dismutase based phylogeny of pathogenic fungi

Fréalle, Émilie; Noël, Christophe; Nolard, N.; Symoens, Françoise; Felipe, M. S. S.; Dei‐Cas, Eduardo; Camus, Daniel; Viscogliosi, Éric; Delhaès, Laurence

doi:10.1016/j.ympev.2006.05.001

Cited by 32 publications

(22 citation statements)

References 54 publications

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“…The eukaryotes are separated into three common kingdoms (Fungi, Animalia, and Plantae). The fungi show a dichotomy into cytosolic and mitochondrial forms of their MnSODs as previously reported (Frealle et al , 2006). The fungal taxa presented are all Ascomycetes and conform to the relationships presented in earlier works constructing a 6-gene phylogeny (James et al , 2006).…”

Section: Resultssupporting

confidence: 69%

Searching for convergent evolution in manganese superoxidase dismutase using hydrophobic cluster analysis

Xiang

Zhang

et al. 2014

Genet. Mol. Biol.

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There are numerous examples of convergent evolution in nature. Major ecological adaptations such as flight, loss of limbs in vertebrates, pesticide resistance, adaptation to a parasitic way of life, etc., have all evolved more than once, as seen by their analogous functions in separate taxa. But what about protein evolution? Does the environment have a strong enough influence on intracellular processes that enzymes and other functional proteins play, to evolve similar functional roles separately in different organisms? Manganese Superoxide Dismutase (MnSOD) is a manganesedependant metallo-enzyme which plays a crucial role in protecting cells from anti-oxidative stress by eliminating reactive (superoxide) oxygen species. It is a ubiquitous housekeeping enzyme found in nearly all organisms. In this study we compare phylogenies based on MnSOD protein sequences to those based on scores from Hydrophobic Cluster Analysis (HCA). We calculated HCA similarity values for each pair of taxa to obtain a pair-wise distance matrix. A UPGMA tree based on the HCA distance matrix and a common tree based on the primary protein sequence for MnSOD was constructed. Differences between these two trees within animals, enterobacteriaceae, planctomycetes and cyanobacteria are presented and cited as possible examples of convergence. We note that several residue changes result in changes in hydrophobicity at positions which apparently are under the effect of positive selection.

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Section: Resultssupporting

confidence: 69%

Searching for convergent evolution in manganese superoxidase dismutase using hydrophobic cluster analysis

Xiang

Zhang

et al. 2014

Genet. Mol. Biol.

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“…FKS1 sequences have been reported for F. solani (14) and related Fusarium species (29; www.broad.mit.edu) but not for any Scedosporium species. Phylogenetic analysis suggests a close relationship between these two genera (11). Therefore, PCR primers for amplifying S. prolificans FKS1 were designed based on Fusarium FKS1 sequences that are conserved among ascomycetous molds, as deduced from BLAST analyses, and with the consideration of S. prolificans codon usage.…”

Section: Resultsmentioning

confidence: 99%

Role for Fks1 in the Intrinsic Echinocandin Resistance of Fusarium solani as Evidenced by Hybrid Expression in Saccharomyces cerevisiae

Katiyar

Edlind

2009

Antimicrob Agents Chemother

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The opportunistic mold Fusarium solani is intrinsically resistant to cell wall synthesis-inhibiting echinocandins (ECs), including caspofungin and micafungin. Mutations that confer acquired EC resistance in Saccharomyces cerevisiae and other normally susceptible yeast species have been mapped to the Fks1 gene; among these is the mutation of residue 639 from Phe to Tyr (F639Y) within a region designated hot spot 1. Fks1 sequence analysis identified the equivalent of Y639 in F. solani as well as in Scedosporium prolificans, another intrinsically EC-resistant mold. To test its role in intrinsic EC resistance, we constructed Fks1 hybrids in S. cerevisiae that incorporate F. solani hot spot 1 and flanking residues. Hybrid construction was accomplished by a PCR-based method that was validated by studies with Fks1 sequences from EC-susceptible Aspergillus fumigatus and paired EC-susceptible and -resistant Candida glabrata isolates. In support of our hypothesis, hybrid Fks1 incorporating F. solani hot spot 1 conferred significantly reduced EC susceptibility, 4-to 8-fold less than that of wild-type S. cerevisiae and 8-to 32-fold less than that of the same hybrid with an F639 mutation. We propose that Fks1 sequences represent determinants of intrinsic EC resistance in Fusarium and Scedosporium species and, potentially, other fungi.

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“…Like most eukaryotes, fungi possess cytosolic Cu/ZnSODs and homotetrameric MnSODs, which were found early in mitochondrial matrix [2] and later in cytosol [3] and peroxisome [4]. Indeed, two different MnSODs co-exist in the cytosol and mitochondria of human pathogen Candida albicans [5] and several fungi [6].…”

Section: Introductionmentioning

confidence: 99%

Additive Contributions of Two Manganese-Cored Superoxide Dismutases (MnSODs) to Antioxidation, UV Tolerance and Virulence of Beauveria bassiana

et al. 2012

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The biocontrol potential of entomopathogenic fungi against arthropod pests depends on not only their virulence to target pests but tolerance to outdoor high temperature and solar UV irradiation. Two Beauveria bassiana superoxide dismutases (SODs), BbSod2 and BbSod3, were characterized as cytosolic and mitochondrial manganese-cored isoenzymes (MnSODs) dominating the total SOD activity of the fungal entomopathogen under normal growth conditions. To probe their effects on the biocontrol potential of B. bassiana, ΔBbSod2, ΔBbSod3, and three hairpin RNA-interfered (RNAi) mutants with the transcripts of both BbSod2 and BbSod3 being suppressed by 91–97% were constructed and assayed for various phenotypic parameters in conjunction with ΔBbSod2/BbSod2, ΔBbSod3/BbSod3 and wild-type (control strains). In normal cultures, the knockout and RNAi mutants showed significant phenotypic alterations, including delayed sporulation, reduced conidial yields, and impaired conidial quality, but little change in colony morphology. Their mycelia or conidia became much more sensitive to menadione or H2O2-induced oxidative stress but had little change in sensitivity to the hyperosmolarity of NaCl and the high temperature of 45°C. Accompanied with the decreased antioxidative capability, conidial tolerances to UV-A and UV-B irradiations were reduced by 16.8% and 45.4% for ΔBbSod2, 18.7% and 44.7% for ΔBbSod3, and ∼33.7% and ∼63.8% for the RNAi mutants, respectively. Their median lethal times (LT50s) against Myzus persicae apterae, which were topically inoculated under a standardized spray, were delayed by 18.8%, 14.5% and 37.1%, respectively. Remarkably, the effects of cytosolic BbSod2 and mitochondrial BbSod3 on the phenotypic parameters important for the fungal bioncontrol potential were additive, well in accordance with the decreased SOD activities and the increased superoxide levels in the knockout and RNAi mutants. Our findings highlight for the first time that the two MnSODs co-contribute to the biocontrol potential of B. bassiana by mediating cellular antioxidative response.

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Manganese superoxide dismutase based phylogeny of pathogenic fungi

Cited by 32 publications

References 54 publications

Searching for convergent evolution in manganese superoxidase dismutase using hydrophobic cluster analysis

Searching for convergent evolution in manganese superoxidase dismutase using hydrophobic cluster analysis

Role for Fks1 in the Intrinsic Echinocandin Resistance of Fusarium solani as Evidenced by Hybrid Expression in Saccharomyces cerevisiae

Additive Contributions of Two Manganese-Cored Superoxide Dismutases (MnSODs) to Antioxidation, UV Tolerance and Virulence of Beauveria bassiana

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