Manganese uptake mediated by the NRAMP-type transporter MntH is required for acid tolerance in Listeria monocytogenes

Wu, Jialun; McAuliffe, Olivia; O’Byrne, Conor

doi:10.1016/j.ijfoodmicro.2023.110238

Cited by 6 publications

(7 citation statements)

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“…In C2 for 268M, more proteins related to oxidative stress were identified, as the MntA protein is linked to SOD by MntH; these proteins are expressed in this experimental condition to cope with low pH and high osmolarity (Zeng et al, 2019 ). In fact, manganese (Mn) transporters (MntH and MntB) were found to be highly expressed after exposure to mild acid stress (pH 5) (Wu et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

A comprehensive investigation of protein expression profiles in L. monocytogenes exposed to thermal abuse, mild acid, and salt stress conditions

D'Onofrio,

Schirone,

Krasteva

et al. 2023

Front. Microbiol.

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Preventing L. monocytogenes infection is crucial for food safety, considering its widespread presence in the environment and its association with contaminated RTE foods. The pathogen's ability to persist under adverse conditions, for example, in food processing facilities, is linked to virulence and resistance mechanisms, including biofilm formation. In this study, the protein expression patterns of two L. monocytogenes 1/2a strains, grown under environmental stressors (mild acidic pH, thermal abuse, and high concentration of NaCl), were investigated. Protein identification and prediction were performed by nLC-ESI-MS/MS and nine different bioinformatic software programs, respectively. Gene enrichment analysis was carried out by STRING v11.05. A total of 1,215 proteins were identified, of which 335 were non-cytosolic proteins and 265 were immunogenic proteins. Proteomic analysis revealed differences in protein expression between L. monocytogenes strains in stressful conditions. The two strains exhibited unique protein expression profiles linked to stress response, virulence, and pathogenesis. Studying the proteomic profiles of such microorganisms provides information about adaptation and potential treatments, highlighting their genetic diversity and demonstrating the utility of bioinformatics and proteomics for a broader analysis of pathogens.

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Section: Discussionmentioning

confidence: 99%

A comprehensive investigation of protein expression profiles in L. monocytogenes exposed to thermal abuse, mild acid, and salt stress conditions

D'Onofrio,

Schirone,

Krasteva

et al. 2023

Front. Microbiol.

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“…The acid sensitive and acid intolerant CC2 strain 1381 is closely related to the chromosomally sequenced reference strain LI0521 [34], facilitating an investigation into the genetic basis of these unusual phenotypes. There are 4 genes intact in strain LI0521 that are truncated by the presence of premature stop codon (PMSC) in strain 1381, including mntH [34]. Among the other three is a putative RofA-like transcriptional regulator, located downstream of and oriented convergently with the acid resistance operon gadT2D2 (Fig 1).…”

Section: Identification Of a Rofa-like Regulator That Positively Infl...mentioning

confidence: 99%

“…One strain identified in that study, a lineage I strain designated 1381, displayed reduced acid tolerance (no growth at pH 4.9) and reduced acid resistance (poor survival at pH 2.3) although there was no evidence of mutations affecting the SigB system [33]. We have recently shown that the growth defect in strain 1381 at low pH, but not the poor survival phenotype (at pH 2.3), is caused by a mutation in the mntH gene, which encodes a manganese transporter belonging to the NRAMP family [34]. However, this mutation does not account for the decreased acid resistance in this strain [34].…”

Section: Introductionmentioning

confidence: 99%

“…We have recently shown that the growth defect in strain 1381 at low pH, but not the poor survival phenotype (at pH 2.3), is caused by a mutation in the mntH gene, which encodes a manganese transporter belonging to the NRAMP family [34]. However, this mutation does not account for the decreased acid resistance in this strain [34]. Thus, the genotype of this strain could help provide new insights into the mechanisms underpinning acid resistance in L. monocytogenes.…”

Section: Introductionmentioning

confidence: 99%

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A novel RofA-family transcriptional regulator, GadR, controls the development of acid resistance inListeria monocytogenes

McAuliffe

O’Byrne

2023

Preprint

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Stomach acid provides a significant innate barrier to the entry of the food-borne pathogen Listeria monocytogenes into the human gastrointestinal tract. A key determinant of acid resistance in this bacterium is the conserved glutamate decarboxylase system, GadD2 (encoded by the gadT2D2 operon), which helps to maintain the intracellular pH during exposure to gastric acid. In this study, we identified a premature stop codon in a gene located immediately downstream of the gadT2D2 operon that was highly linked to an acid sensitive phenotype. When this open reading frame was restored through homologous recombination an acid resistant phenotype resulted. Through a series of genetic, transcriptomic and survival experiments we established that this gene, which we designated gadR, encodes a transcriptional regulator of the gadT2D2 operon. GadR belongs to the RofA family of regulators, primarily found in the streptococci, where they are involved in regulating virulence. The data further showed that gadR plays a critical role in the development of acid resistance in response to mild acid exposure, a response that is known as the adaptive acid tolerance response (ATR). A deletion analysis of the gadT2D2 promoter region identified two 18bp palindromic sequences that are required for the GadR-mediated induction of gadT2D2, suggesting that they act as binding sites for GadR. Overall, this study uncovers a new RofA-like regulator of acid resistance in L. monocytogenes that plays a significant role in both growth phase-dependent and ATR-mediated acid resistance and accounts for previously observed strain-to-strain differences in survival at low pH. The findings have important implications for understanding the behavior of L. monocytogenes in acidic environments and identify a potential target for improved control of this important pathogen.

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“…Even with the well-established knowledge and matured expertise in good manufacturing practice and hygienic design, L. monocytogenes persists as a recurring food contamination problem in recent years [ 2 ]. At present, it is still a real challenge for the food industries to combat the foodborne pathogens like L. monocytogenes , thanks to the bug's built-in tolerance to adverse environmental conditions, such as extreme temperatures (as low as 1 °C), acidic pH condition (at pH 2.3), as well as to the formation of resistant biofilms the bug developed in niches it sticks around [ [2] , [3] , [4] ]. The need of safe yet natural antibacterial agents against L. monocytogenes is on rising.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a novel circular bacteriocin from Bacillus velezensis 1-3, and its mode of action against Listeria monocytogenes

Zhang,

Zhao,

Tang

et al. 2024

Heliyon

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Manganese uptake mediated by the NRAMP-type transporter MntH is required for acid tolerance in Listeria monocytogenes

Cited by 6 publications

References 68 publications

A comprehensive investigation of protein expression profiles in L. monocytogenes exposed to thermal abuse, mild acid, and salt stress conditions

A comprehensive investigation of protein expression profiles in L. monocytogenes exposed to thermal abuse, mild acid, and salt stress conditions

A novel RofA-family transcriptional regulator, GadR, controls the development of acid resistance inListeria monocytogenes

Characterization of a novel circular bacteriocin from Bacillus velezensis 1-3, and its mode of action against Listeria monocytogenes

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