Manipulating cell fate: dynamic control of cell behaviors on functional platforms

Li, Wen; Yan, Zhengqing; Ren, Jinsong; Qu, Xiaogang

doi:10.1039/c8cs00053k

Cited by 128 publications

(94 citation statements)

References 336 publications

(406 reference statements)

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“…These determine the cell morphology, alter cellular behavior, maintain the tissue integrity, and provide location signals to guide migration and differentiation . The predefined pattern of an artificial extracellular matrix for cell adhesion is the cell′s behavior including growth and differentiation . Cell adhesion to substrates is mainly mediated by integrin receptors, which recognize the RGD (Arg‐Gly‐Asp) tripeptide motif in extracellular matrix proteins, mediate intracellular signal transduction, and regulate actin cytoskeleton dynamics .…”

Section: Applicationsmentioning

confidence: 99%

“…[42] The predefined pattern of an artificial extracellularm atrix for cell adhesion is the cell'sb ehaviori ncluding growth and differentiation. [43] Cell adhesion to substrates is mainly mediated by integrin receptors, which recognize the RGD (Arg-Gly-Asp) tripeptide motif in extracellular matrix proteins, mediate intracellular signal transduction, and regulate actin cytoskeleton dynamics. [44] Therefore, RGD is the most commonly used receptor recognition module to have been explored for quantitative regulation of cell adhesion.…”

Section: Regulation Of Cell Adhesionmentioning

confidence: 99%

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Engineering Cell‐Surface Receptors with DNA Nanotechnology for Cell Manipulation

et al. 2019

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Cell‐surface receptors play pivotal roles in the regulation of cell fate. Molecular engineering of cell‐surface receptors enables control of cell signaling and manipulation of cell behavior in a user‐defined way. Currently, the development of chemical‐biological approaches for non‐genetic engineering and regulation of membrane receptors is attracting significant interest. Recent research advances in functional nucleic acids and DNA nanotechnology have made it possible to use DNA as a new and promising molecular toolkit for controlling receptor‐mediated signaling and cell fates. In this minireview we summarize the advances in the use of DNA nanotechnology for the spatiotemporal regulation of cell receptors and highlight practical applications in manipulating cell functions including cell adhesion, cell–cell contact, cell migration, and cellular immunity. We also provide a perspective on the potential of and challenges facing DNA‐based receptor engineering in future applications of cell manipulation and cell‐based therapy.

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Section: Applicationsmentioning

confidence: 99%

Section: Regulation Of Cell Adhesionmentioning

confidence: 99%

Engineering Cell‐Surface Receptors with DNA Nanotechnology for Cell Manipulation

et al. 2019

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“…Namely, those systems are simplified into single differentiation behaviors without cell–cell interactions, as compared to the complicated progression of stem cell development. [ 2,10 ] As such, new developments in the dynamic regulation of the complex behaviors of stem cells with spatiotemporal controllability will be essential in developing novel dynamic biological tissue systems, and crucial for the full realization of their diverse applications.…”

Section: Introductionmentioning

confidence: 99%

“…[8,9,10] Several stimuli-responsive methods, including applied electrical and magnetic fields, changes in pH and temperature, introduction of light and enzymes, have been applied to modulate the physicochemical properties of culture substrates. [2,3,9,11,12] Specifically, these methods can be used to modify culture substrate surface properties (e.g., hydrophilicity/hydrophobicity), mechanical properties (e.g., elasticity), and scaffold topographies. [2,9,10,12,13] However, there are few examples that present controlled, simultaneous regulation of these biologically relevant cues in order to influence stem cells at the more complex tissue level.…”

Section: Introductionmentioning

confidence: 99%

4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation

et al. 2020

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As the most versatile and promising cell source, stem cells have been studied in regenerative medicine for two decades. Currently available culturing techniques utilize a 2D or 3D microenvironment for supporting the growth and proliferation of stem cells. However, these culture systems fail to fully reflect the supportive biological environment in which stem cells reside in vivo, which contain dynamic biophysical growth cues. Herein, a 4D programmable culture substrate with a self‐morphing capability is presented as a means to enhance dynamic cell growth and induce differentiation of stem cells. To function as a model system, a 4D neural culture substrate is fabricated using a combination of printing and imprinting techniques keyed to the different biological features of neural stem cells (NSCs) at different differentiation stages. Results show the 4D culture substrate demonstrates a time‐dependent self‐morphing process that plays an essential role in regulating NSC behaviors in a spatiotemporal manner and enhances neural differentiation of NSCs along with significant axonal alignment. This study of a customized, dynamic substrate revolutionizes current stem cell therapies, and can further have a far‐reaching impact on improving tissue regeneration and mimicking specific disease progression, as well as other impacts on materials and life science research.

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“…With the development of modern cell biology, the scope of cell researches has enlarged from the early interest in cell structures, functions, and working mechanisms to many rising star fields, such as exploring of life origin, biotechnologies, and cell factories . Artificial cells not only have the expectation for substituting natural cells to investigate life origin in the future but also have the potential for the basic cell biology research for the establishment of artificial cell factory . Furthermore, artificial cells exhibit more advantages in some ways than natural cell because many problems, such as fragile to be handled, complicated to be analyzed and easy apoptosis, are as shadows following natural cell research …”

Section: Introductionmentioning

confidence: 99%

Microfluidics for Biosynthesizing: from Droplets and Vesicles to Artificial Cells

Xie

Xiong

et al. 2019

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Fabrication of artificial biomimetic materials has attracted abundant attention. As one of the subcategories of biomimetic materials, artificial cells are highly significant for multiple disciplines and their synthesis has been intensively pursued. In order to manufacture robust “alive” artificial cells with high throughput, easy operation, and precise control, flexible microfluidic techniques are widely utilized. Herein, recent advances in microfluidic‐based methods for the synthesis of droplets, vesicles, and artificial cells are summarized. First, the advances of droplet fabrication and manipulation on the T‐junction, flow‐focusing, and coflowing microfluidic devices are discussed. Then, the formation of unicompartmental and multicompartmental vesicles based on microfluidics are summarized. Furthermore, the engineering of droplet‐based and vesicle‐based artificial cells by microfluidics is also reviewed. Moreover, the artificial cells applied for imitating cell behavior and acting as bioreactors for synthetic biology are highlighted. Finally, the current challenges and future trends in microfluidic‐based artificial cells are discussed. This review should be helpful for researchers in the fields of microfluidics, biomaterial fabrication, and synthetic biology.

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Manipulating cell fate: dynamic control of cell behaviors on functional platforms

Abstract: We review the recent advances and new horizons in the dynamic control of cell behaviors on functional platforms and their applications.

Cited by 128 publications

References 336 publications

Engineering Cell‐Surface Receptors with DNA Nanotechnology for Cell Manipulation

Engineering Cell‐Surface Receptors with DNA Nanotechnology for Cell Manipulation

4D Self‐Morphing Culture Substrate for Modulating Cell Differentiation

Microfluidics for Biosynthesizing: from Droplets and Vesicles to Artificial Cells

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