Manipulation of the morphogenetic pathways of tobacco explants by oligosaccharins

Van, K. Tran Thanh; Toubart, P.; Cousson, A.; Darvill, Alan G.; Gollin, David J.; Chelf, Paulanne; Albersheim, Peter

doi:10.1038/314615a0

Cited by 201 publications

(24 citation statements)

References 25 publications

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“…Similar to oligoalginate, degraded chitosan was also reported to have a growth‐promotion effect for plants. Thanh Van et al [33] and Vasyokova et al [6] have reported that oligosaccharides have an effect on growth promotion and regulation of morphogenesis in plants. Irradiated chitosan showed a strong effect on the increase in dry weight and root length of rice and barley as well as wet biomass of orchid in tissue culture.…”

Section: Discussionmentioning

confidence: 99%

Biological effect of irradiated chitosan on plants in vitro

Luân

Nagasawa

et al. 2005

Biotech and App Biochem

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For degradation of chitosan, chitosan with an 80% degree of deacetylation and a weight-average molecular mass (Mw) of approx. 48 kDa was irradiated with gamma-rays at doses up to 200 kGy in a 10% (w/v) solution. The Mw of chitosan was reduced from 48 to 9.1 kDa by irradiation. The characteristics of irradiated chitosan were analysed by using Fourier-transform IR spectroscopy and an elemental analyser. The amino group was found to be stable, whereas the C-O-C group decreased with increase in the dose. The product of chitosan irradiated at 100 kGy with an Mw of approx. 16 kDa showed the strongest growth promotion effect on plants in vitro. For shoot culture, supplementation with irradiated chitosan increased the fresh biomass of shoot clusters (7.2-17.0%) as well as the shoot multiplication rate (17.9-69.0%) for Chrysanthemum morifolium (florist's chrysanthemum), Limonium latifolium (limonium or sea-lavender), Eustoma grandiflorum (lisianthus, tulip gentian or Texas bluebell) and Fragaria ananassa (modern garden strawberry). The optimum concentrations of irradiated chitosan were found to be approx. 70-100 mg/l for chrysanthemum, 50-100 mg/l for lisianthus and 30-100 mg/l for limonium. For the plantlet culture, the optimum concentrations were found to be approx. 100 mg/l for chrysanthemum, 30 mg/l for lisianthus, 40 mg/l for limonium and 50 mg/l for strawberry. Supplementation with optimum concentrations of irradiated chitosan resulted in a significant increase in the fresh biomass (68.1% for chrysanthemum, 48.5% for lisianthus, 53.6% for limonium and 26.4% for strawberry), shoot height (19.4% for chrysanthemum, 16.5% for lisianthus, 33.9% for limonium and 25.9% for strawberry) and root length (40.6% for chrysanthemum, 66.9% for lisianthus, 23.4% for limonium and 22.6% for strawberry). In addition, treatment with irradiated chitosan enhanced the activity of chitosanase in treated plants and also improved the survival ratio and growth of the transferred plantlets acclimatized for 10-30 days under greenhouse conditions.

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Section: Discussionmentioning

confidence: 99%

Biological effect of irradiated chitosan on plants in vitro

Luân

Nagasawa

et al. 2005

Biotech and App Biochem

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“…The majority of the identified oligosac charins have been described as stimulators or inhibitors of stem segment elongation [9,55,56]. Significantly higher variety of responses is yielded in the study of morphogen esis in in vitro cultures [5,29,39], but this method is very time consuming. Definitely, the currently used test sys tems do not cover all possible varieties of plant cell responses to different oligosaccharins.…”

Section: Problems Of Research On Oligosaccharinsmentioning

confidence: 99%

“…The effects of oligosaccharins depends on their concen tration, but also on the concentration and type of hor mone present in the culture medium; this reveals the rela tionship between the mechanisms of action of these groups of physiologically active compounds [5,15,35,37,40,115]. Most of the works demonstrating the interaction of oligosaccharins and hormones were focused on the analysis of their effect on plant growth and morphogene sis.…”

Section: General Information About Physiologically Active Oligosacchamentioning

confidence: 99%

Plant oligosaccharides — outsiders among elicitors?

Larskaya

Горшкова

2015

Biochemistry Moscow

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This review substantiates the need to study the plant oligoglycome. The available information on oligosaccharins - physiologically active fragments of plant cell wall polysaccharides - is summarized. The diversity of such compounds in chemical composition, origin, and proved biological activity is highlighted. At the same time, plant oligosaccharides can be considered as outsiders among elicitors of various natures in research intensity of recent decades. This review discusses the reasons for such attitude towards these regulators, which are largely connected with difficulties in isolation and identification. Together with that, approaches are suggested whose potentials can be used to study oligosaccharins. The topics of oligosaccharide metabolism in plants, including the ways of formation, transport, and inactivation are presented, together with data on biological activity and interaction with plant hormones. The current viewpoints on the mode of oligosaccharin action - perception, signal transduction, and possible "targets" - are considered. The potential uses of such compounds in medicine, food industry, agriculture, and biotechnology are discussed.

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“…For example, Hu et al 13 ) reported that agar may contain some growth inhibitory substances. From a different point of view, Tran Than Van et al 14 ) reported that Gellan gum contain stimulative substances for cell growth and cell differentiation or active substances similar to oligosaccharins. In spite of these papers, the real reasons for the effects of Gellan gum are not yet clear.…”

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confidence: 99%