Mannose in vaccine delivery

Nahar, Ummey Jannatun; Tóth, István; Skwarczyński, Mariusz

doi:10.1016/j.jconrel.2022.09.038

Cited by 32 publications

(13 citation statements)

References 144 publications

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“…The incorporation of a mannose-based ligand into liposome formulations has been previously reported as being effective in promoting CTL responses [ 40 , 41 , 42 ]. We designed our ligands ( M1 – M3 ) to have different spacer lengths between the mannose and lipidic moieties, where the importance of these spacers has been previously reported [ 3 , 43 , 44 , 45 ]. We have also demonstrated the mechanism of mannose-receptor-mediated uptake [ 46 ].…”

Section: Resultsmentioning

confidence: 99%

Liposomal Formulations of a Polyleucine–Antigen Conjugate as Therapeutic Vaccines against Cervical Cancer

et al. 2023

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Human papilloma virus (HPV) is responsible for all cases of cervical cancer. While prophylactic vaccines are available, the development of peptide-based vaccines as a therapeutic strategy is still under investigation. In comparison with the traditional and currently used treatment strategies of chemotherapy and surgery, vaccination against HPV is a promising therapeutic option with fewer side effects. A peptide derived from the HPV-16 E7 protein, called 8Qm, in combination with adjuvants showed promise as a therapeutic vaccine. Here, the ability of polymerized natural amino acids to act as a self-adjuvating delivery system as a therapeutic vaccine was investigated for the first time. Thus, 8Qm was conjugated to polyleucine by standard solid-phase peptide synthesis and self-assembled into nanoparticles or incorporated in liposomes. The liposome bearing the 8Qm conjugate significantly increased mice survival and decreased tumor growth after a single immunization. Further, these liposomes eradicated seven-day-old well-established tumors in mice. Dendritic cell (DC)-targeting moieties were introduced to further enhance vaccine efficacy, and the newly designed liposomal vaccine was tested in mice bearing 11-day-old tumors. Interestingly, these DCs-targeting moieties did not significantly improve vaccine efficacy, whereas the simple liposomal formulation of 8Qm-polyleucine conjugate was still effective in tumor eradication. In summary, a peptide-based anticancer vaccine was developed that stimulated strong cellular immune responses without the help of a classical adjuvant.

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Section: Resultsmentioning

confidence: 99%

Liposomal Formulations of a Polyleucine–Antigen Conjugate as Therapeutic Vaccines against Cervical Cancer

et al. 2023

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“…For this study, we selected a variety of known and previously examined immune-stimulating moieties (TMC, lipoPEI, DEAE-dextran) [ 22 , 26 , 32 ], as well as untested OTMC. Chitosan derivatives (e.g., TMC) are known immune stimulators [ 33 ], while lipidated sugars often demonstrate adjuvanting abilities, especially once incorporated into liposomes [ 34 , 35 , 36 , 37 , 38 ]. Therefore, we hypothesized that a lipidated analog of TMC (OTMC) may also be an effective immune stimulator.…”

Section: Discussionmentioning

confidence: 99%

The Development of Surface-Modified Liposomes as an Intranasal Delivery System for Group A Streptococcus Vaccines

et al. 2023

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Intranasal vaccine administration can overcome the disadvantages of injectable vaccines and present greater efficiency for mass immunization. However, the development of intranasal vaccines is challenged by poor mucosal immunogenicity of antigens and the limited availability of mucosal adjuvants. Here, we examined a number of self-adjuvanting liposomal systems for intranasal delivery of lipopeptide vaccine against group A Streptococcus (GAS). Among them, two liposome formulations bearing lipidated cell-penetrating peptide KALA and a new lipidated chitosan derivative (oleoyl-quaternized chitosan, OTMC) stimulated high systemic antibody titers in outbred mice. The antibodies were fully functional and were able to kill GAS bacteria. Importantly, OTMC was far more effective at stimulating antibody production than the classical immune-stimulating trimethyl chitosan formulation. In a simple physical mixture, OTMC also enhanced the immune responses of the tested vaccine, without the need for a liposome delivery system. The adjuvanting capacity of OTMC was further confirmed by its ability to stimulate cytokine production by dendritic cells. Thus, we discovered a new immune stimulant with promising properties for mucosal vaccine development.

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“…1B) such as bacteria, yeasts and parasites by identifying polysaccharides on the cell wall. 55 Recognition of specific receptors triggers downstream signals and induces dynamic changes in cellular behavior, reflecting the anti-inflammatory effects of polysaccharides.…”

Section: Effects Of Different Structures On the Bioactivity Of Polysa...mentioning

confidence: 99%

The effect of structure and preparation method on the bioactivity of polysaccharides from plants and fungi

Yang

Ren

et al. 2022

Food Funct.

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Mannose in vaccine delivery

Cited by 32 publications

References 144 publications

Liposomal Formulations of a Polyleucine–Antigen Conjugate as Therapeutic Vaccines against Cervical Cancer

Liposomal Formulations of a Polyleucine–Antigen Conjugate as Therapeutic Vaccines against Cervical Cancer

The Development of Surface-Modified Liposomes as an Intranasal Delivery System for Group A Streptococcus Vaccines

The effect of structure and preparation method on the bioactivity of polysaccharides from plants and fungi

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