Mannose receptor of Epinephelus coioides exerts antiviral activity against red-spotted grouper nervous necrosis virus and regulates apoptosis and inflammation

Zhang, Menglan; Lu, Zhijie; Tang, Meizhen; Pan, Gan; Zhao, Lijuan; Qin, Zhendong; Lin, Li

doi:10.1016/j.aquaculture.2021.737264

Cited by 4 publications

(2 citation statements)

References 56 publications

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“…Contrary to the most phagocytosis genes, the expression of most NF-κB pathway genes and inflammation genes was significantly increased in the LvMR knockdown shrimp. It is consistent with that reported in the EcMR from orange-spotted grouper Epinephelus coioides and mannose receptors on human alveolar macrophage, where MRs knockdown lead to the increase of proinflammatory cytokines [26,27]. These results indicate that LvMR might play an important role in the anti-inflammatory process.…”

Section: Discussionsupporting

confidence: 91%

A Mannose Receptor from Litopenaeus vannamei Involved in Innate Immunity by Pathogen Recognition and Inflammation Regulation

Guo

Liu

Hao

et al. 2023

IJMS

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Mannose receptor, as a member of the C-type lectin superfamily, is a non-canonical pattern recognition receptor that can internalize pathogen-associated ligands and activate intracellular signaling. Here, a mannose receptor gene, LvMR, was identified from the Pacific white shrimp Litopenaeus vannamei. LvMR encoded a signal peptide, a fibronectin type II (FN II) domain, and two carbohydrate-recognition domains (CRDs) with special EPS and FND motifs. LvMR transcripts were mainly detected in the hepatopancreas, and presented a time-dependent response after pathogen challenge. The recombinant LvMR (rLvMR) could bind to various PAMPs and agglutinate microorganisms in a Ca2+-dependent manner with strong binding ability to D-mannose and N-acetyl sugars. The knockdown of LvMR enhanced the expression of most NF-κB pathway genes, inflammation and redox genes, while it had no obvious effect on the transcription of most phagocytosis genes. Moreover, the knockdown of LvMR caused an increase in reactive oxygen species (ROS) content and inducible nitric oxide synthase (iNOS) activity in the hepatopancreas after Vibrio parahaemolyticus infection. All these results indicate that LvMR might perform as a PRR in immune recognition and a negative regulator of inflammation during bacterial infection.

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Section: Discussionsupporting

confidence: 91%

A Mannose Receptor from Litopenaeus vannamei Involved in Innate Immunity by Pathogen Recognition and Inflammation Regulation

Guo

Liu

Hao

et al. 2023

IJMS

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“…This indicates that MR is involved in antibacterial defense of crayfish ( 69 ). The immune importance of MR genes has been demonstrated in aquatic animals like grass carp, crayfish, and Epinephelus coioides ( 68 – 70 ), although little research has been done in R. philippinarum. Therefore, the function of the MR gene in the immune response of R. philippinarum to V. anguillarum requires further study.…”

Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms Underlying Vibrio Tolerance in Ruditapes philippinarum Revealed by Comparative Transcriptome Profiling

et al. 2022

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The clam Ruditapes philippinarum is an important species in the marine aquaculture industry in China. However, in recent years, the aquaculture of R. philippinarum has been negatively impacted by various bacterial pathogens. In this study, the transcriptome libraries of R. philippinarum showing different levels of resistance to challenge with Vibrio anguillarum were constructed and RNA-seq was performed using the Illumina sequencing platform. Host immune factors were identified that responded to V. anguillarum infection, including C-type lectin domain, glutathione S-transferase 9, lysozyme, methyltransferase FkbM domain, heat shock 70 kDa protein, Ras-like GTP-binding protein RHO, C1q, F-box and BTB/POZ domain protein zf-C2H2. Ten genes were selected and verified by RT-qPCR, and nine of the gene expression results were consistent with those of RNA-seq. The lectin gene in the phagosome pathway was expressed at a significantly higher level after V. anguillarum infection, which might indicate the role of lectin in the immune response to V. anguillarum. Comparing the results from R. philippinarum resistant and nonresistant to V. anguillarum increases our understanding of the resistant genes and key pathways related to Vibrio challenge in this species. The results obtained here provide a reference for future immunological research focusing on the response of R. philippinarum to V. anguillarum infection.

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RNA-Seq and metabolomic analysis reveal dynamic response mechanism to hypoxia in the pearl oyster Pinctada fucata martensii

Luo,

Liao,

Yang

et al. 2024

Regional Studies in Marine Science

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Mannose receptor of Epinephelus coioides exerts antiviral activity against red-spotted grouper nervous necrosis virus and regulates apoptosis and inflammation

Cited by 4 publications

References 56 publications

A Mannose Receptor from Litopenaeus vannamei Involved in Innate Immunity by Pathogen Recognition and Inflammation Regulation

A Mannose Receptor from Litopenaeus vannamei Involved in Innate Immunity by Pathogen Recognition and Inflammation Regulation

Molecular Mechanisms Underlying Vibrio Tolerance in Ruditapes philippinarum Revealed by Comparative Transcriptome Profiling

RNA-Seq and metabolomic analysis reveal dynamic response mechanism to hypoxia in the pearl oyster Pinctada fucata martensii

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