Mantle Transcriptome Provides Insights into Biomineralization and Growth Regulation in the Eastern Oyster (Crassostrea virginica)

Zhang, Dan

doi:10.1007/s10126-021-10088-7

Cited by 14 publications

(5 citation statements)

References 92 publications

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“…This finding suggests that these polymorphisms may confer disease resistance through transcriptional regulation of PRRs and downstream immune signaling pathways. In the Eastern oysters, resistance to Perkinsus marinus is influenced by polymorphism in the promoter region of a serine protease inhibitor (107), and shell growth is regulated by both transcriptional modulation and functional polymorphism, two separate mechanisms that may act on different sets of genes (108).…”

Section: Discussionmentioning

confidence: 99%

Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas

Yao

Guan

et al. 2022

Front. Immunol.

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The Ostreid herpesvirus 1 (OsHV-1) is a lethal pathogen of the Pacific oyster (Crassostrea gigas), an important aquaculture species. To understand the genetic architecture of the defense against the pathogen, we studied genomic variations associated with herpesvirus-caused mortalities by pooled whole-genome resequencing of before and after-mortality larval samples as well as dead and surviving adults from a viral challenge. Analysis of the resequencing data identified 5,271 SNPs and 1,883 genomic regions covering 3,111 genes in larvae, and 18,692 SNPs and 28,314 regions covering 4,863 genes in adults that were significantly associated with herpesvirus-caused mortalities. Only 1,653 of the implicated genes were shared by larvae and adults, suggesting that the antiviral response or resistance in larvae and adults involves different sets of genes or differentiated members of expanded gene families. Combined analyses with previous transcriptomic data from challenge experiments revealed that transcription of many mortality-associated genes was also significantly upregulated by herpesvirus infection confirming their importance in antiviral response. Key immune response genes especially those encoding antiviral receptors such as TLRs and RLRs displayed strong association between variation in regulatory region and herpesvirus-caused mortality, suggesting they may confer resistance through transcriptional modulation. These results point to previously undescribed genetic mechanisms for disease resistance at different developmental stages and provide candidate polymorphisms and genes that are valuable for understanding antiviral immune responses and breeding for herpesvirus resistance.

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Section: Discussionmentioning

confidence: 99%

Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas

Yao

Guan

et al. 2022

Front. Immunol.

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“…Mucins are secreted by epithelial cells and aid biomineralization of vertebrate bones, teeth, and cartilage (Midura and Hascall 1996). Mucin-like genes have been previously identified in molluscan mantle tissue, and are hypothesized to aid shell biomineralization (Marin et al 2000; Marin and Luquet 2005; Zeng and Guo 2022). In C. atrasolea , we identified two mucin-like genes, CaSMP9 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Spatial-temporal expression analysis of lineage-restricted shell matrix proteins reveals shell field regionalization and distinct cell populations in the slipper snailCrepidula atrasolea

Lopez-Anido

Batzel

Ramírez

et al. 2023

Preprint

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Molluscs are one of the most morphologically diverse clades of metazoans, exhibiting an immense diversification of calcium carbonate structures, such as the shell. Biomineralization of the calcified shell is dependent on shell matrix proteins (SMPs). While SMP diversity is hypothesized to drive molluscan shell diversity, we are just starting to unravel SMP evolutionary history and biology. Here we leveraged two complementary model mollusc systems, Crepidula fornicata and Crepidula atrasolea, to determine the lineage-specificity of 185 Crepidula SMPs. We found that 95% of the adult C. fornicata shell proteome belongs to conserved metazoan and molluscan orthogroups, with molluscan-restricted orthogroups containing half of all SMPs in the shell proteome. The low number of C. fornicata-restricted SMPs contradicts the generally-held notion that an animal's biomineralization toolkit is dominated by novel genes. Next, we selected a subset of lineage-restricted SMPs for spatial-temporal analysis using in situ hybridization chain reaction (HCR) during larval stages in C. atrasolea. We found that 12 out of 18 SMPs analyzed are expressed in the shell field. Notably, these genes are present in 5 expression patterns, which define at least three distinct cell populations within the shell field. These results represent the most comprehensive analysis of gastropod SMP evolutionary age and shell field expression patterns to date. Collectively, these data lay the foundation for future work to interrogate the molecular mechanisms and cell fate decisions underlying molluscan mantle specification and diversification.

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“…Thus, this pathway may similarly exhibit genetic variation that supports rapid adaptation in a range of putatively sensitive species. Interestingly, a recent study identified SNPs in HSP70, as well as tyrosinase and chitinase genes, to be associated with the rate of shell growth in the oyster Crassostrea virginica, potentially corroborating the involvement of these genes in low pH adaptation (Zeng and Guo, 2021).…”

Section: A Core Set Of Genes Underlies Adaptive Potential To Low Phmentioning

confidence: 89%

Molecular basis of ocean acidification sensitivity and adaptation in Mytilus galloprovincialis

et al. 2022

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Mantle Transcriptome Provides Insights into Biomineralization and Growth Regulation in the Eastern Oyster (Crassostrea virginica)

Cited by 14 publications

References 92 publications

Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas

Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas

Spatial-temporal expression analysis of lineage-restricted shell matrix proteins reveals shell field regionalization and distinct cell populations in the slipper snailCrepidula atrasolea

Molecular basis of ocean acidification sensitivity and adaptation in Mytilus galloprovincialis

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