Many ways, one microorganism: Several approaches to study Malassezia in interactions with model hosts

Ehemann, Kevin; Mantilla, María Juliana; Mora, Felipe; Rios-Navarro, Andrea; Torres, Mario; Ramírez, Adriana Marcela Celis

doi:10.1371/journal.ppat.1010784

Cited by 5 publications

(3 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The emergence of drug-resistant microorganisms has increased exponentially despite the discovery of new therapeutic antibiotics [35][36][37][38][39][40]. Identifying new phytopreparations that can destroy single planktonic cells and microbial communities in biofilms, thereby preventing antibiotic resistance, an important development [13,[41][42][43][44][45][46].…”

Section: Discussionmentioning

confidence: 99%

A rabbit model of ear otitis established using the Malassezia pachydermatis strain C23 from dogs

Sachivkina,

Karamyan,

Petrukhina

et al. 2023

Vet World

View full text Add to dashboard Cite

Background and Aim: Fungal infections are a growing problem for both humans and animals due to the emergence of pathogenic strains resistant to modern antifungal treatments. To evaluate the efficacy of new antifungal drugs, it is essential to develop animal models that demonstrate typical responses to both the infection (pathogenesis and clinical course) and to the treatment, including adverse effects. In this study, we established a rabbit otitis model by infection of an aggressive multidrug-resistant strain from dogs, Malassezia pachydermatis C23, with no need for concomitant immunosuppression. Materials and Methods: Twenty healthy adult male gray giant rabbits (1 year old, 5.5 kg) were inoculated once with M. pachydermatis C23 at 108 colony-forming units/mL. We observed the clinical signs of the disease and collected ear smears and blood samples every 5 days. Results: The infection progressed rapidly and exhibited characteristic clinical signs without spontaneous recovery for at least 1 month. In fact, substantial deterioration was observed as evidenced by blood parameters. Conclusion: This rabbit otitis model established using an aggressive drug-resistant fungus strain without immunosuppression could prove valuable for testing novel antifungal agents. Keywords: in vivo model, Malassezia pachydermatis, otitis media, rabbit.

show abstract

Section: Discussionmentioning

confidence: 99%

A rabbit model of ear otitis established using the Malassezia pachydermatis strain C23 from dogs

Sachivkina,

Karamyan,

Petrukhina

et al. 2023

Vet World

View full text Add to dashboard Cite

show abstract

“…Other species are related to fungemia, and recently, some authors have suggested an association of fungemia with Crohn’s disease [ 8 ] and Parkinson’s disease [ 9 ]. Furthermore, to try to understand why these yeasts transition from beneficial to opportunistic behavior, different approaches using in vitro or in vivo experimental models have been performed through several methodologies, such as omics tools, to elucidate the interaction between Malassezia and its host; these studies have been recently summarized [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Why Do These Yeasts Smell So Good? Volatile Organic Compounds (VOCs) Produced by Malassezia Species in the Exponential and Stationary Growth Phases

et al. 2023

Self Cite

View full text Add to dashboard Cite

Malassezia synthesizes and releases volatile organic compounds (VOCs), small molecules that allow them to carry out interaction processes. These lipid-dependent yeasts belong to the human skin mycobiota and are related to dermatological diseases. However, knowledge about VOC production and its function is lacking. This study aimed to determine the volatile profiles of Malassezia globosa, Malassezia restricta, and Malassezia sympodialis in the exponential and stationary growth phases. The compounds were separated and characterized in each growth phase through headspace solid-phase microextraction (HS-SPME) and gas chromatography–mass spectrometry (GC–MS). We found a total of 54 compounds, 40 annotated. Most of the compounds identified belong to alcohols and polyols, fatty alcohols, alkanes, and unsaturated aliphatic hydrocarbons. Unsupervised and supervised statistical multivariate analyses demonstrated that the volatile profiles of Malassezia differed between species and growth phases, with M. globosa being the species with the highest quantity of VOCs. Some Malassezia volatiles, such as butan-1-ol, 2-methylbutan-1-ol, 3-methylbutan-1-ol, and 2-methylpropan-1-ol, associated with biological interactions were also detected. All three species show at least one unique compound, suggesting a unique metabolism. The ecological functions of the compounds detected in each species and growth phase remain to be studied. They could interact with other microorganisms or be an important clue in understanding the pathogenic role of these yeasts.

show abstract

“…In medical practice, this type of skin pathology is diagnosed based on the patient's clinical characteristics, and empirical management is given, so etiological isolation is not generally performed. It is important to highlight that these treatments require prolonged use of antifungal agents, which in turn takes the risk of leading to antifungal resistance development (13)(14)(15). Resistance mechanisms are not fully elucidated.…”

mentioning

confidence: 99%

In vitro sensitivity of Malassezia furfur isolates from HIV-positive and negative patients to antifungal agents

Ehemann,

Contreras,

Celis-Ramírez

2023

biomedica

Self Cite

View full text Add to dashboard Cite

Introduction. Malassezia is a lipophilic and lipid-dependent yeast genus belonging to the skin microbiota of humans and other animals. However, due to dysbiosis processes or other factors in the host, this yeast can cause different pathologies, ranging from skin diseases, such as seborrheic dermatitis, to fungemia. Isolation of Malassezia furfur has been reported in HIV-positive patients with or without skin lesions. Due to its opportunistic nature and its variable resistance to antifungal compounds, it is relevant to know the Malassezia sensitivity profiles.Objective. To determine the sensitivity to different antifungal agents, of clinical isolates of M. furfur obtained from HIV-positive or negative patients, with or without seborrheic dermatitis.Materials and methods. Assessment of isolates sensitivity to itraconazole, voriconazole, fluconazole, and amphotericin B was performed by two techniques: (1) Broth microdilution using Clinical and Laboratory Standards Institute (CLSI) protocol M27-A3 with modifications; and (2) agar tests using Etest®.Results. Isolates obtained from HIV patients showed an increase in the minimum inhibitory concentration of fluconazole, voriconazole, and amphotericin B, compared with those of non-HIV patients. Itraconazole was the antifungal with the lowest minimum inhibitory concentration (MIC) in most isolates.Conclusion. We observed differences in the sensitivity profiles of M. furfur isolates according to the context of the patient. High MIC of antifungals like fluconazole, commonly used for treating pathologies caused by Malassezia, were identified.

show abstract

Many ways, one microorganism: Several approaches to study Malassezia in interactions with model hosts

Cited by 5 publications

References 81 publications

A rabbit model of ear otitis established using the Malassezia pachydermatis strain C23 from dogs

A rabbit model of ear otitis established using the Malassezia pachydermatis strain C23 from dogs

Why Do These Yeasts Smell So Good? Volatile Organic Compounds (VOCs) Produced by Malassezia Species in the Exponential and Stationary Growth Phases

In vitro sensitivity of Malassezia furfur isolates from HIV-positive and negative patients to antifungal agents

Contact Info

Product

Resources

About