Map kinase MGV1: a potential shared control point of butenolide and deoxynivalenol biosynthesis In Fusarium graminearum

Rampitsch, Christof; Leung, Winnie; Blackwell, Barbara A.; Subramaniam, Rajagopal

doi:10.2478/v10129-011-0031-0

Cited by 6 publications

(12 citation statements)

References 12 publications

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“…5 ). With Mgv1 previously being shown to be important in fungal hyphal growth and pathogenicity ( 67 , 68 ), these results are consistent with the observed reduction in chemotropic responses ( Fig. 1 ) ( 24 ).…”

Section: Discussionsupporting

confidence: 90%

Fusarium graminearumSte3 G-Protein Coupled Receptor: A Mediator of Hyphal Chemotropism and Pathogenesis

Sharma

Sridhar

Blackman

et al. 2022

mSphere

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Fusarium head blight of wheat, caused by the filamentous fungus Fusarium graminearum , leads to devastating global food shortages and economic losses. Fungal hyphal chemotropism has been shown to be a major contributor to host-pathogen interactions. Here, the role of the opposite mating type GPCR, Ste3, is characterized with respect to F. graminearum chemotropism and pathogenicity. These findings contribute to our understanding of the mechanisms underlying fungal chemotropism and pathogenesis.

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Section: Discussionsupporting

confidence: 90%

Fusarium graminearumSte3 G-Protein Coupled Receptor: A Mediator of Hyphal Chemotropism and Pathogenesis

Sharma

Sridhar

Blackman

et al. 2022

mSphere

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“…A member of the butenolide cluster FGSG_08077 [51] was less abundant in agmatine and was shown to be regulated by tri6 in F. sporothrichiodes and under opposite regulation with DON in the wild type and the mgvkinase1 knockout mutant [52].…”

Section: Metabolic and Structural Changes Induced By Agmatinementioning

confidence: 99%

A Fusarium graminearum strain-comparative proteomic approach identifies regulatory changes triggered by agmatine

Pasquali

Serchi

Cocco

et al. 2016

Journal of Proteomics

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“…MAPKs are essential for the transduction and amplification of environmental signals to the transcriptional regulatory network. Disruption mutants of the MAPK, MGV1 , indicate that this gene is involved in the regulation of DON biosynthesis in F. graminearum [ 21 ]. The transcription factor, TRI6, regulates the expression of genes of the trichothecene biosynthesis genes ( TRI genes) [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

“…F. graminearum (NRRL29169) was the wild-type (WT) and parental strain used for the construction of the transgenic strains. The construction of ∆mgv1 and MGV1 Oex6 strains have been previously described [ 21 , 22 ]. Briefly, overexpression was achieved by replacing the native promoter of MGV1 with the constitutive promoter (p GPD ) of the glyceraldehyde 3-phosphate dehydrogenase gene from A. nidulans [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Epistatic Relationship between MGV1 and TRI6 in the Regulation of Biosynthetic Gene Clusters in Fusarium graminearum

Shostak

Fundora

Blackman

et al. 2023

JoF

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Genetic studies have shown that the MAP kinase MGV1 and the transcriptional regulator TRI6 regulate many of the same biosynthetic gene clusters (BGCs) in Fusarium graminearum. This study sought to investigate the relationship between MGV1 and TRI6 in the regulatory hierarchy. Transgenic F. graminearum strains constitutively expressing MGV1 and TRI6 were generated to address both independent and epistatic regulation of BGCs by MGV1 and TRI6. We performed a comparative transcriptome analysis between axenic cultures grown in nutrient-rich and secondary metabolite-inducing conditions. The results indicated that BGCs regulated independently by Mgv1 included genes of BGC52, whereas genes uniquely regulated by TRI6 included the gene cluster (BGC49) that produces gramillin. To understand the epistatic relationship between MGV1 and TRI6, CRISPR/Cas9 was used to insert a constitutive promoter to drive TRI6 expression in the Δmgv1 strain. The results indicate that BGCs that produce deoxynivalenol and fusaoctaxin are co-regulated, with TRI6 being partially regulated by MGV1. Overall, the findings from this study indicate that MGV1 provides an articulation point to differentially regulate various BGCs. Moreover, TRI6, embedded in one of the BGCs provides specificity to regulate the expression of the genes in the BGC.

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Map kinase MGV1: a potential shared control point of butenolide and deoxynivalenol biosynthesis In Fusarium graminearum

Cited by 6 publications

References 12 publications

Fusarium graminearumSte3 G-Protein Coupled Receptor: A Mediator of Hyphal Chemotropism and Pathogenesis

Fusarium graminearumSte3 G-Protein Coupled Receptor: A Mediator of Hyphal Chemotropism and Pathogenesis

A Fusarium graminearum strain-comparative proteomic approach identifies regulatory changes triggered by agmatine

Epistatic Relationship between MGV1 and TRI6 in the Regulation of Biosynthetic Gene Clusters in Fusarium graminearum

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