MAP2 is localized to the dendrites of hippocampal neurons which develop in culture

Cáceres, Alfredo; Banker, Gary; Steward, Oswald; Binder, Lester I.; Payne, Michael R.

doi:10.1016/0165-3806(84)90167-6

Cited by 308 publications

(180 citation statements)

References 13 publications

Supporting

Mentioning

167

Contrasting

Order By: Relevance

“…Within each pyramidal neuron, the MAP2 was localized primarily to the soma and dendrites, whereas the NF200k protein was distributed predominantly to the axon (not shown). Consistent with previous in vivo and in vitro reports (Bernhardt and Matus, 1984;Caceres et al, 1984;De Camilli et (Willins et al, 1997;Jakab and Goldman-Rakic, 1998;Cornea-Hebert et al, 1999). Within the dendrites of cortical pyramidal neurons, the majority of the 5-HT 2A receptors are found intracellularly in close association with MAP-1A (Cornea-Hebert et al, 2002), with a small population of 5-HT 2A receptors also present in dendritic spines and post-synaptic densities (Miner et al, 2003).…”

Section: Resultssupporting

confidence: 90%

The PDZ-binding domain is essential for the dendritic targeting of 5-HT2A serotonin receptors in cortical pyramidal neurons in vitro

et al. 2003

View full text Add to dashboard Cite

Section: Resultssupporting

confidence: 90%

The PDZ-binding domain is essential for the dendritic targeting of 5-HT2A serotonin receptors in cortical pyramidal neurons in vitro

et al. 2003

View full text Add to dashboard Cite

“…These neurons project one long and branching axon and several short, straight dendrites. In cultured hippocampal pyramidal neurons, the single, long process has been positively identified as the axon by its immunoreactivity to tau antibody and lack of immunoreactivity to MAP2 antibody; dendrites stain heavily with MAP2 antibody and lack tau (Caceres et al, 1984(Caceres et al, , 1986. In preliminary experiments we confirmed this relationship between axonal and dendritic morphology and compartmentalization of MAP2 and tau in pyramidal neurons under the conditions of the present study.…”

Section: Methodssupporting

confidence: 77%

“…These neurons provided a particularly useful model for such studies because the axon and dendrites can be unambiguously identified by morphological and immunocytological criteria (Caceres et al, 1984(Caceres et al, , 1986M. P. Mattson, unpublished observations).…”

mentioning

confidence: 99%

Outgrowth-regulating actions of glutamate in isolated hippocampal pyramidal neurons

1988

View full text Add to dashboard Cite

The present study examined the effects of glutamate on the outgrowth of dendrites and axons in isolated hippocampal pyramidal-like neurons in cell culture. During the first day of culture the survival and outgrowth of these neurons was unaffected by high concentrations (up to 1 nM) of glutamate, quisqualic acid (QA), kainic acid (KA), and N- methyl-D-aspartic acid. Beginning on day 2 of culture high levels of glutamate, KA and QA were toxic to the majority of pyramidal neurons, while subtoxic levels of these agents caused a well-defined, dose- dependent, sequence of effects on dendritic outgrowth. At increasing concentrations of glutamate, QA, and KA, the following events were observed: (1) dendritic outgrowth rates were reduced, while axonal elongation rates were unaffected; (2) dendritic length was reduced, while axons continued to grow; (3) dendrites regressed dramatically, and axonal outgrowth rate was reduced. These dendrite-specific effects of glutamate were apparently mediated at the growth cones since focal application of glutamate to individual dendritic growth cones resulted in suppression of growth cone activity and a regression of the dendrite; axons were unaffected by focal glutamate application. Pharmacological tests using glutamate receptor agonists and antagonists demonstrated that receptors of the KA/QA type mediated the glutamate effects on outgrowth and survival. The calcium channel blocker Co2+ prevented both glutamate neurotoxicity and glutamate-induced dendritic regression. Ionophore A23187 and elevations in extracellular K+ levels each caused a dose-dependent series of outgrowth and survival responses similar to those caused by glutamate. Taken together, these results indicate that activation of glutamate receptors leads to the opening of voltage-dependent calcium channels; the resulting increases in calcium influx lead to the observed alterations in dendritic outgrowth and neuronal survival.

show abstract

“…Generally, MAP2 is associated with somatodendritic regions of neurons (Caceres et al, 1984). GFAP is a common intermediate filament protein that is restricted to astroglia (Bignami et al, 1972).…”

Section: Effects Of Added Astroglia On Cell Numbermentioning

confidence: 99%

Added astroglia promote greater synapse density and higher activity in neuronal networks

2007

View full text Add to dashboard Cite

Astroglia are known to potentiate individual synapses, but their contribution to networks is unclear.Here we examined the effect of adding either astroglia or media conditioned by astroglia on entire networks of rat hippocampal neurons cultured on microelectrode arrays. Added astroglia increased spontaneous spike rates nearly two-fold and glutamate-stimulated spiking by six-fold, with desensitization eliminated for bath addition of 25 μM glutamate. Astrocyte-conditioned medium partly mimicked the effects of added astroglia. Bursting behavior was largely unaffected by added astroglia except with added glutamate. Addition of the GABA A receptor antagonist bicuculline also increased spike rates but with more subtle differences between networks without or with added astroglia. This indicates that networks without added astroglia were inhibited greatly. In all conditions, the log-log distribution of spike rates fit well to linear distributions over three orders of magnitude. Networks with added astroglia shifted consistently toward higher spike rates. Immunostaining for GFAP revealed a linear increase with added astroglia, which also increased neuronal survival. The increased spike rates with added astroglia correlated with a 1.7-fold increase in immunoreactive synaptophysin puncta, and increases of six-fold for GABA Aβ , two-fold for NMDA-R1 and two-fold for Glu-R1 puncta, with receptor clustering that indicated synaptic scaling. Together, these results indicate that added astroglia increase the density of synapses and receptors, and facilitate higher spike rates for many elements in the network. These effects are reproduced by glia-conditioned media, with the exception of glutamate-mediated transmission.

show abstract

MAP2 is localized to the dendrites of hippocampal neurons which develop in culture

Cited by 308 publications

References 13 publications

The PDZ-binding domain is essential for the dendritic targeting of 5-HT2A serotonin receptors in cortical pyramidal neurons in vitro

The PDZ-binding domain is essential for the dendritic targeting of 5-HT2A serotonin receptors in cortical pyramidal neurons in vitro

Outgrowth-regulating actions of glutamate in isolated hippocampal pyramidal neurons

Added astroglia promote greater synapse density and higher activity in neuronal networks

Contact Info

Product

Resources

About