MAPKs in the early steps of senescence implemEMTation

Anerillas, Carlos; Altés, Gisela; Gorospe, Myriam

doi:10.3389/fcell.2023.1083401

Cited by 4 publications

(1 citation statement)

References 142 publications

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“…Several other studies [ 40 – 42 ] have shown that this pathway is involved in cell proliferation, differentiation, and apoptosis, making it an important signaling pathway. In mammals, MAPK can be translated as “mitogen-activated protein kinase” Regulating cell proliferation, differentiation, movement, and survival is one of the important pathways affecting testis development and spermatogenesis [ 43 ]. This suggests that the MAPK signaling pathway plays a critical role in regulating rooster testis development, spermatogenesis, and sperm quality.…”

Section: Discussionmentioning

confidence: 99%

Dietary restriction promote sperm remodeling in aged roosters based on transcriptome analysis

Liang,

He,

Zhu

et al. 2024

BMC Genomics

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Background The breeder rooster has played a pivotal role in poultry production by providing high-quality semen. Typically, fertility peaks between 30 and 40 weeks of age and then declines rapidly from 45 to 55 weeks of age. Research into improving fertility in aging roosters is essential to extend their productive life. While progress has been made, enhancing fertility in aging roosters remains a significant challenge. Methods To identify the genes related to promoting sperm remodeling in aged Houdan roosters, we combined changes in testis and semen quality with transcriptome sequencing (RNA-seq) to analyze the synchrony of semen quality and testis development. In this study, 350-day-old Houdan breeder roosters were selected for RNA-seq analysis in testis tissues from induced molting roosters (D group) and non-induced molting roosters (47DG group). All analyses of differentially expressed genes (DEGs) and functional enrichment were performed. Finally, we selected six DEGs to verify the accuracy of the sequencing by qPCR. Results Compared with the 47DG group, sperm motility (P < 0.05), sperm density (P < 0.01), and testis weight (P < 0.05) were significantly increased in roosters in the D group. Further RNA-seq analysis of the testis between the D group and 47DG group identified 61 DEGs, with 21 up-regulated and 40 down-regulated. Functional enrichment analysis showed that the DEGs were primarily enriched in the cytokine-cytokine receptor interaction, Wnt signaling pathway, MAPK signaling pathway, TGF-β signaling pathway, and focal adhesion pathway. The qRT-PCR results showed that the expression trend of these genes was consistent with the sequencing results. WNT5A, FGFR3, AGTR2, TGFβ2, ROMO1, and SLC26A7 may play a role in testis development and spermatogenesis. This study provides fundamental data to enhance the reproductive value of aging roosters.

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Section: Discussionmentioning

confidence: 99%

Dietary restriction promote sperm remodeling in aged roosters based on transcriptome analysis

Liang,

He,

Zhu

et al. 2024

BMC Genomics

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The YAP–TEAD complex promotes senescent cell survival by lowering endoplasmic reticulum stress

Anerillas,

Mazan-Mamczarz,

Herman

et al. 2023

Nat Aging

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Sublethal cell damage can trigger a complex adaptive program known as senescence, characterized by growth arrest, resistance to apoptosis, and a senescence-associated secretory phenotype (SASP). As senescent cells accumulating in aging organs are linked to many ageassociated diseases, senotherapeutic strategies are actively sought to eliminate them. Here, a whole-genome CRISPR knockout screen revealed that proteins in the YAP-TEAD pathway influenced senescent cell viability. Accordingly, treating senescent cells with a drug that inhibited this pathway, Verteporfin (VPF), selectively triggered apoptotic cell death and derepressed DDIT4, in turn inhibiting mTOR. Reducing mTOR function in senescent cells diminished endoplasmic reticulum (ER) biogenesis, causing ER stress and apoptosis due to high demands on ER function by the SASP. Importantly, VPF treatment decreased senescent cell numbers in the organs of old mice and mice exhibiting doxorubicin-induced senescence. We present a novel senolytic strategy that eliminates senescent cells by hindering ER activity required for SASP production.

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Dietary Restriction Promote Sperm Remodeling in Aged Roosters Based on Transcriptome Analysis

liang,

he,

zhu

et al. 2024

Preprint

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Background: The breeder rooster has played an important role in poultry production by providing high-quality semen. In general, the fertility peaks of breeder roosters between 30 and 40 weeks of age and declines rapidly from 45 to 55 weeks of age. The exploration of improving fertility in aging roosters would help to extend the useful life of roosters. At present, researchers have made some progress, but improving fertility in aging roosters has become an important issue that needs to be addressed. Methods: To identify the genes related to promoting sperm remodeling in aged Houdan roosters, we combined changes in testis and semen quality with transcriptome sequencing (RNA-seq) to analyze the synchrony of semen quality and testis development. In this study, 350-day-old Houdan breeder roosters were selected for RNA-seq analysis in testis tissues from induced molting roosters (D group) and non-induced molting roosters (47DG group). All analyses of differentially expressed genes (DEGs) and functional enrichment were performed. Finally, we selected six DEGs to verify the accuracy of the sequencing by qPCR. Results: Sperm motility, sperm density, and testis weight were significantly increased in roosters in the fasted-induced molting group compared with the control group (P < 0.05). Further RNA-seq analysis of the testis between the D group and 47DG group identified 61 DEGs, with 21 up-regulated and 40 down-regulated. Functional enrichment analysis showed that the DEGs were primarily enriched in the cytokine-cytokine receptor interaction, Wnt signaling pathway, MAPK signaling pathway, TGF-β signaling pathway, and focal adhesion pathway. The qRT-PCR results showed that the expression trend of these genes was consistent with the sequencing results. WNT5A, FGFR3, AGTR2, TGFβ2, ROMO1, and SLC26A7 may play a role in testis development and spermatogenesis. This study provides fundamental data to enhance the reproductive value of aging roosters.

show abstract

MAPKs in the early steps of senescence implemEMTation

Cited by 4 publications

References 142 publications

Dietary restriction promote sperm remodeling in aged roosters based on transcriptome analysis

Dietary restriction promote sperm remodeling in aged roosters based on transcriptome analysis

The YAP–TEAD complex promotes senescent cell survival by lowering endoplasmic reticulum stress

Dietary Restriction Promote Sperm Remodeling in Aged Roosters Based on Transcriptome Analysis

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