Mapping 45S and 5S ribosomal genes in chromosomes of Anostomidae fish species (Ostariophysi, Characiformes) from different Amazonian water types

Barros, Lucas Caetano de; Galetti, Pedro Manoel; Feldberg, Eliana

doi:10.1007/s10750-015-2583-8

Cited by 9 publications

(12 citation statements)

References 65 publications

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“…Intraspecific variations were observed in the chromosomal location and quantity of heterochromatin blocks, which were mainly located in pericentromeric regions in L.vittatus and terminal positions of chromosomes in L.friderici . These heterochromatin distribution in the chromosomes have also been observed for other anostomids (Pereira et al 2002, Aguilar and Galetti Jr 2008, Barros et al 2017b). Satellite DNA is one of the components of heterochromatin, which is also enriched in other dispersed repeated elements, including transposons (Mazzuchelli and Martins 2009, Vicari et al 2010).…”

Section: Discussionsupporting

confidence: 78%

“…Anostomids usually present only one pair of 45S rDNA (Martins and Galetti Jr 1999), being a common characteristic of this group. Previous studies observed polymorphisms in the number of 45S rDNA sites in Leporinustaeniatus Lütken, 1875, Leporinustrifasciatus Steindachner, 1876, Rhytiodusmicrolepis Kner, 1858 and Schizodonfasciatus Spix & Agassiz, 1829 (Barros et al 2017b). In the present study, although this was also observed, differences in the chromosomal position of 45S rDNA were additionally observed between species, with signals in the terminal region of the p arm for L.friderici and in the q arm for L.vittatus .…”

Section: Discussionmentioning

confidence: 93%

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Comparative cytogenetics among Leporinus friderici and Leporellus vittatus populations (Characiformes, Anostomidae): focus on repetitive DNA elements

Dulz¹,

Lorscheider²,

Nascimento³

et al. 2019

CCG

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Anostomidae are a neotropical fish family rich in number of species. Cytogenetically, they show a conserved karyotype with 2n = 54 chromosomes, although they present intraspecific/interspecific variations in the number and chromosomal location of repetitive DNA sequences. The aim of the present study was to perform a comparative description of the karyotypes of two populations of Leporinusfriderici Bloch, 1794 and three populations of Leporellusvittatus Valenciennes, 1850. We used conventional cytogenetic techniques allied to fluorescence in situ hybridization, using 18S ribosomal DNA (rDNA) and 5S rDNA, a general telomere sequence for vertebrates (TTAGGG)n and retrotransposon (RTE) Rex1 probes. The anostomids in all studied populations presented 2n = 54 chromosomes, with a chromosome formula of 32m + 22sm for L.friderici and 28m + 26sm for L.vittatus. Variations in the number and location of the 5S and 18S rDNA chromosomal sites were observed between L.friderici and L.vittatus populations and species. Accumulation of Rex1 was observed in the terminal region of most chromosomes in all populations, and telomere sequences were located just on all ends of the 54 chromosomes in all populations. The intraspecific and intergeneric chromosomal changes occurred in karyotype differentiation, indicating that minor chromosomal rearrangements had present in anostomid species diversification.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 93%

Comparative cytogenetics among Leporinus friderici and Leporellus vittatus populations (Characiformes, Anostomidae): focus on repetitive DNA elements

Dulz¹,

Lorscheider²,

Nascimento³

et al. 2019

CCG

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“…2 ). The maximum numbers of 45S sites were found in the Amazonian fish Schizodon fasciatus (54/2C, de Barros et al 2017 ) and the brook trout Salvelinus fontinalis (50/2C, Fujiwara et al 1998 ). In mammals, the maximum number of 45S loci was identified in Mus pahari (rodent) having 42 sites/2C (Britton-Davidian et al 2012 ).…”

Section: Resultsmentioning

confidence: 99%

Evolutionary trends in animal ribosomal DNA loci: introduction to a new online database

et al. 2017

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Ribosomal DNA (rDNA) loci encoding 5S and 45S (18S-5.8S-28S) rRNAs are important components of eukaryotic chromosomes. Here, we set up the animal rDNA database containing cytogenetic information about these loci in 1343 animal species (264 families) collected from 542 publications. The data are based on in situ hybridisation studies (both radioactive and fluorescent) carried out in major groups of vertebrates (fish, reptiles, amphibians, birds, and mammals) and invertebrates (mostly insects and mollusks). The database is accessible online at www.animalrdnadatabase.com. The median number of 45S and 5S sites was close to two per diploid chromosome set for both rDNAs despite large variation (1–74 for 5S and 1–54 for 45S sites). No significant correlation between the number of 5S and 45S rDNA loci was observed, suggesting that their distribution and amplification across the chromosomes follow independent evolutionary trajectories. Each group, irrespective of taxonomic classification, contained rDNA sites at any chromosome location. However, the distal and pericentromeric positions were the most prevalent (> 75% karyotypes) for 45S loci, while the position of 5S loci was more variable. We also examined potential relationships between molecular attributes of rDNA (homogenisation and expression) and cytogenetic parameters such as rDNA positions, chromosome number, and morphology.Electronic supplementary materialThe online version of this article (10.1007/s00412-017-0651-8) contains supplementary material, which is available to authorized users.

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“…Up to now, a heteromorphic ZZ/ZW system of sex chromosomes has been found in only one of the anostomid species that occur in the Amazon basin, M. trifasciatus [Barros et al, 2017]. Given this, the present study used specific probes obtained from the chromosomal microdissection of the W chromosome and crossed FISH (cross-FISH) to verify the similarity of the W sequence of M. trifasciatus with those of the species with undifferentiated sex chromosomes.…”

mentioning

confidence: 98%

Differentiation and Evolution of the W Chromosome in the Fish Species of <b><i>Megaleporinus </i></b>(Characiformes, Anostomidae)

Barros

Piscor

Parise-Maltempi

et al. 2018

Sex Dev

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The W chromosome of Megaleporinus trifasciatus was isolated in order to analyze its behavior in the karyotype of this and other species of the family, including forms with differentiated and undifferentiated sex chromosomes. The chromosome was microdissected, and the WMt probe was prepared for the chromosome painting procedure. M. trifasciatus was also cross-hybridized (cross-FISH) using existing probes available for M. macrocephalus (WMm) and M. elongatus (WMe). Two Leporinus species and Semaprochilodus taeniurus, representing a clade close to the Anostomidae, were also cross-hybridized with the objective to better understand the evolution of the sex chromosomes. In the metaphase of female M. trifasciatus, the WMt probe highlighted the whole long arm of the W chromosome and a small, distal portion of the long arm of the Z chromosome. In males, the probe highlighted the distal portion of the long arm of the Z chromosomes. The hybridization of female M. trifasciatus with the WMe and WMm probes revealed a pattern similar to that encountered using the WMt probe. The WMt, WMm, and WMe probes revealed broad similarities among the species of the genus Megaleporinus, which has a ZZ/ZW system of sex chromosomes, with only minor alterations becoming apparent when analyzed separately.

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Mapping 45S and 5S ribosomal genes in chromosomes of Anostomidae fish species (Ostariophysi, Characiformes) from different Amazonian water types

Cited by 9 publications

References 65 publications

Comparative cytogenetics among Leporinus friderici and Leporellus vittatus populations (Characiformes, Anostomidae): focus on repetitive DNA elements

Comparative cytogenetics among Leporinus friderici and Leporellus vittatus populations (Characiformes, Anostomidae): focus on repetitive DNA elements

Evolutionary trends in animal ribosomal DNA loci: introduction to a new online database

Differentiation and Evolution of the W Chromosome in the Fish Species of <b><i>Megaleporinus </i></b>(Characiformes, Anostomidae)

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