Mapping a gene conferring resistance to Wheat yellow mosaic virus in European winter wheat cultivar ‘Ibis’ (Triticum aestivum L.)

Nishio, Zenta; Kojima, Hisao; Hayata, Akiyo; Iriki, Norio; Tabiki, Tadashi; Ito, Masaru; Yamauchi, Hiroaki; Murray, T. D.

doi:10.1007/s10681-010-0229-5

Cited by 29 publications

(25 citation statements)

References 22 publications

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“…Ningmai 13 and Yangfumai 4 both had high resistance to wheat yellow mosaic virus as Ningmai 9. Ningmai 9 possessed the wheat yellow mosaic virus resistant QTL with the same allele on chromosome 2DL and 3BS as previous reports [ 17 , 18 ]. Ningmai 13 and Yangfumai 4 had the identical alleles on the chromosome regions with Ningmai 9.…”

Section: Discussionsupporting

confidence: 82%

Genetic Contribution of Ningmai 9 Wheat to Its Derivatives Evaluated by Using SNP Markers

Jiang

Zhang

2016

International Journal of Genomics

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Founder parent usually plays an important role in wheat breeding. Ningmai 9 is a soft wheat variety with good performance in yield, quality, and resistance to wheat disease. Therefore it serves as an important commercial variety and founder parent in middle and lower Yangtze River of China. To date, 20 new cultivars have been developed from Ningmai 9 and released to wheat production in the last 10 years. In this study, the 90K iSELECT ILLUMINA chip was used to analyze the genotype of Ningmai 9 and its 17 derivatives. The genetic similarity coefficients between Ningmai 9 and its derivatives were more than 0.7 except for Yangfumai 4. Neighbor-Joining analysis showed that Yangfumai 4 had the largest genetic distance from Ningmai 9 in all derivatives. There was a great difference for the same allele ratio in either derivatives or chromosomes, though the average values of the same allele ratio in genomes A, B, and D were close to each other. The phenotypic difference in Ningmai 9, Ningmai 13, and Yangfumai 4 was consistent with their difference in genetic background by comparing previous reported QTLs. Some hot chromosome regions were found and might be used for marker assisted selection in wheat breeding.

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Section: Discussionsupporting

confidence: 82%

Genetic Contribution of Ningmai 9 Wheat to Its Derivatives Evaluated by Using SNP Markers

Jiang

Zhang

2016

International Journal of Genomics

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“…The best effective method to control the disease is to grow resistant wheat varieties, but there are few known resistance genes in current wheat cultivars. It has been reported that WYMV resistance in wheat is controlled by one to three genes (Qin et al, 1986) with one major gene being associated with homologous group 2 chromosome (Liu et al, 2005a;Nishio et al, 2010;Zhu et al, 2012). Furthermore, genetic analyses showed that the inheritance of this resistance is complex and influenced by many factors (Zhou et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Durable field resistance to wheat yellow mosaic virus in transgenic wheat containing the antisense virus polymerase gene

Chen¹,

Sun²,

HongYa³

et al. 2013

Plant Biotechnology Journal

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Summary Wheat yellow mosaic virus (WYMV) has spread rapidly and causes serious yield losses in the major wheat‐growing areas in China. Because it is vectored by the fungus‐like organism Polymyxa graminis that survives for long periods in soil, it is difficult to eliminate by conventional crop management or fungicides. There is also only limited resistance in commercial cultivars. In this research, fourteen independent transgenic events were obtained by co‐transformation with the antisense NIb8 gene (the NIb replicase of WYMV) and a selectable gene bar. Four original transgenic lines (N12, N13, N14 and N15) and an offspring line (N12‐1) showed high and durable resistance to WYMV in the field. Four resistant lines were shown to have segregated and only contain NIb8 (without bar) by PCR and herbicide resistance testing in the later generations. Line N12‐1 showed broad‐spectrum resistance to WYMV isolates from different sites in China. After growing in the infested soil, WYMV could not be detected by tissue printing and Western blot assays of transgenic wheat. The grain yield of transgenic wheat was about 10% greater than the wild‐type susceptible control. Northern blot and small RNA deep sequencing analyses showed that there was no accumulation of small interfering RNAs targeting the NIb8 gene in transgenic wheat plants, suggesting that transgene RNA silencing, a common mechanism of virus‐derived disease resistance, is not involved in the process of WYMV resistance. This durable and broad‐spectrum resistance to WYMV in transgenic wheat will be useful for alleviating the damage caused by WYMV.

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“…Additionally, resistant genes against wheat yellow mosaic virus and soil-borne wheat mosaic virus were found on chromosomes 2D and 5D, respectively. 6 , 42 For the fine mapping of these agronomically important genes or QTLs, the number of markers must be increased around the regions of interest. From the polymorphism survey using six varieties, around 6,000 polymorphic sites were detected on the D genome, and some sites have been successfully used to develop markers (data not shown).…”

Section: Discussionmentioning

confidence: 99%

An efficient approach for the development of genome-specific markers in allohexaploid wheat (Triticum aestivum L.) and its application in the construction of high-density linkage maps of the D genome

et al. 2018

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In common wheat, the development of genotyping platforms has been hampered by the large size of the genome, its highly repetitive elements and its allohexaploid nature. However, recent advances in sequencing technology provide opportunities to resolve these difficulties. Using next-generation sequencing and gene-targeting sequence capture, 12,551 nucleotide polymorphisms were detected in the common wheat varieties ‘Hatsumochi’ and ‘Kitahonami’ and were assigned to chromosome arms using International Wheat Genome Sequencing Consortium survey sequences. Because the number of markers for D genome chromosomes in commercially available wheat single nucleotide polymorphism arrays is insufficient, we developed markers using a genome-specific amplicon sequencing strategy. Approximately 80% of the designed primers successfully amplified D genome-specific products, suggesting that by concentrating on a specific subgenome, we were able to design successful markers as efficiently as could be done in a diploid species. The newly developed markers were uniformly distributed across the D genome and greatly extended the total coverage. Polymorphisms were surveyed in six varieties, and 31,542 polymorphic sites and 5,986 potential marker sites were detected in the D genome. The marker development and genotyping strategies are cost effective, robust and flexible and may enhance multi-sample studies in the post-genomic era in wheat.

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Mapping a gene conferring resistance to Wheat yellow mosaic virus in European winter wheat cultivar ‘Ibis’ (Triticum aestivum L.)

Cited by 29 publications

References 22 publications

Genetic Contribution of Ningmai 9 Wheat to Its Derivatives Evaluated by Using SNP Markers

Genetic Contribution of Ningmai 9 Wheat to Its Derivatives Evaluated by Using SNP Markers

Durable field resistance to wheat yellow mosaic virus in transgenic wheat containing the antisense virus polymerase gene

An efficient approach for the development of genome-specific markers in allohexaploid wheat (Triticum aestivum L.) and its application in the construction of high-density linkage maps of the D genome

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