Mapping a gene conferring resistance toPseudocercosporella herpotrichoideson chromosome 4V ofDasypyrum villosumin a wheat background

Yıldırım, Ahmet; Jones, Stephen S.; Murray, Timothy

doi:10.1139/g97-092

Cited by 47 publications

(42 citation statements)

References 14 publications

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“…This species is of great interest as a donor of genes that improve agronomically important traits in cultivated wheats (Qualset et al, 1993). In particular, genes from D. villosum can enhance the expression of yield components , the resistance to such diseases as powdery mildew (De Pace et al, 1988;Qi et al, 1996), brown rust (Mariani, 2003), and eyespot (Murray et al, 1994;Yildrim et al, 1998), the amounts of seed protein (De Pace et al, 1990), and gluten strength (De Pace et al, 2001). On account of this, there has been interest in crossing D. villosum with the cultivated Triticum species.…”

Section: ] × T Aestivum)mentioning

confidence: 99%

Cytogenetics of Triticum × Dasypyrum hybrids and derived lines

Ceccarelli¹,

Mariani²,

Pace³

2005

Cytogenet Genome Res

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Genomic in situ hybridization was used to study Triticum × Dasypyrum wide hybrids and derived lines. A cytogenetic investigation was carried out in progenies of (i) amphiploids derived from T. turgidum var. durum (T. durum; 2n = 14; genomes AABB) × D. villosum (2n = 14; genome VV), (ii) three-parental hybrids (T. durum × D. villosum) × T. aestivum (2n = 42, genomes A′A′B′B′D′D′), and (iii) T. aestivum aneuploid lines carrying D. villosum chromosomes or chromatin. The amphiploids derived from T. durum × D. villosum showed a stable chromosomal constitution, made up of 14 V chromosomes, 14 chromosomes carrying the wheat A genome and 14 chromosomes carrying the B genome. High karyological instability was observed in the progenies of three-parental hybrids ([T. durum × D. villosum] × T. aestivum). Plants having the expected 14 A chromosomes, 14 B chromosomes, 7 D chromosomes, and 7 V chromosomes were rather rare (4.5%). Many progeny plants (45.5%) had the hexaploid wheat genome with 42 chromosomes and lacked any detectable D. villosum chromatin. Other plants (50%) had 14 A chromosomes and 14 B chromosomes, plus variable numbers of D and V chromosomes, the former being better retained than the latter in most cases. Some T. aestivum lines carrying D. villosum chromosomes or chromatin, as the result of addition, substitution, or recombination events or even a combination of these karyological events, were found to be stable. Other lines were unstable, and these lines carried 1V, 3V, or 5V chromosomes or their portions. Substitution or recombination events where 1V chromosomes were involved could concern the homeologous counterparts in both the A and B and D genomes of wheat. No line could be recovered where the shorter arm of 3V chromosomes was present. Changes in the morphology and banding pattern of V chromosomes were observed in hybrids that did not carry the entire D. villosum complement. By comparing the results of our cytogenetic analyses with certain phenotypic characteristics of the lines studied, genes for discrete traits could be assigned to specific V chromosomes or V chromosome arms. From the frequency of V chromosomes that were involved in chromatin exchanges with or substituted for one of their homeologous counterparts in the A, B, and D wheat genomes, it was inferred that D. villosum belongs to the same phyletic lineage as T. urartu (donor of the A genome of wheat) and Aegilops speltoides (B genome), and that Ae. squarrosa (D genome) diverged earlier from D. villosum.

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Section: ] × T Aestivum)mentioning

confidence: 99%

Cytogenetics of Triticum × Dasypyrum hybrids and derived lines

Ceccarelli¹,

Mariani²,

Pace³

2005

Cytogenet Genome Res

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“…This gene has been mapped to the long arm of chromosome 7A (de la Peña et al 1997). A third potential source of resistance, PchD V (Pch3) that has not been tested in commercial wheat cultivars, has been identiWed and mapped to chromosome 4V of Dasypyrum villosum (L.) Candargy (Yildirim et al 1998). Of these three genes, Pch1 has been the most widely used in development of eyespot-resistant wheat cultivars.…”

Section: Introductionmentioning

confidence: 99%

Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1

et al. 2007

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Hansen, Jennifer L.; Zemetra, Robert S.; Santra, Dipak K.; Campbell, Kimberly G.; and Riera-Lizarazu, Oscar, "Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1" (2008 Abstract Wheat is prone to strawbreaker foot rot (eyespot), a fungal disease caused by Oculimacula yallundae and O. acuformis. The most eVective source of genetic resistance is Pch1, a gene derived from Aegilops ventricosa. The endopeptidase isozyme marker allele Ep-D1b, linked to Pch1, has been shown to be more eVective for tracking resistance than DNA-based markers developed to date. Therefore, we sought to identify a candidate gene for Ep-D1 as a basis for a DNA-based marker. Comparative mapping suggested that the endopeptidase loci Ep-D1 (wheat), enp1 (maize), and Enp (rice) were orthologous. Since the product of the maize endopeptidase locus enp1 has been shown to exhibit biochemical properties similar to oligopeptidase B puriWed from E. coli, we reasoned that Ep-D1 may also encode an oligopeptidase B. Consistent with this hypothesis, a sequence-tagged-site (STS) marker, Xorw1, derived from an oligopeptidase B-encoding wheat expressed-sequence-tag (EST) showed complete linkage with Ep-D1 and Pch1 in a population of 254 recombinant inbred lines (RILs) derived from a cross between wheat cultivars Coda and Brundage. Two other STS markers, Xorw5 and Xorw6, and three microsatellite markers (Xwmc14, Xbarc97, and Xcfd175) were also completely linked to Pch1. On the other hand, Xwmc14, Xbarc97, and Xcfd175 showed recombination in the W7984 £ Opata85 RIL population suggesting that recombination near Pch1 is reduced in the Coda/Brundage population. In a panel of 44 wheat varieties with known eyespot reactions, Xorw1, Xorw5, and Xorw6 were 100% accurate in predicting the presence or absence of Pch1 whereas Xwmc14, Xbarc97, and Xcfd175 were less eVective. Thus, linkage mapping and a germplasm survey suggest that the STS markers identiWed here should be useful for indirect selection of Pch1.

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“…Within both species of Dasypyrum , several agronomically important traits, including those of disease resistance, high protein quality, and drought tolerance, have been identified and offer valuable resources for wheat improvement [Gradzielewska, 2006b;De Pace et al, 2011]. D. villosum has been extensively hybridized to wheat, and several disease-resistance genes were successfully transferred [Chen et al, 1995;Yildirim et al, 1998;Qi et al, 2011]. However, very few attempts have been made to transfer useful genes from D. breviaristatum into wheat.…”

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confidence: 99%

A Novel Wheat-Dasypyrum breviaristatum Substitution Line with Stripe Rust Resistance

Guang-rong¹,

Zhao²,

Li³

et al. 2014

Cytogenet Genome Res

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The introduction of genetic variation from wild and cultivated Triticeae species has been a long-standing approach for wheat improvement. Dasypyrumbreviaristatum species harbor novel and agronomically important genes for resistance against multi-fungal diseases. The development of new wheat-D. breviaristatum introgression lines offers chances for the identification of stripe rust resistance gene(s). A wheat line, D11-5, was selected from a cross between wheat line MY11 and wheat-D. breviaristatum partial amphiploid TDH-2. It was characterized by FISH and PCR-based molecular markers. Chromosome counting revealed that the D11-5 line shows a hexaploid set of 2n = 6x = 42 chromosomes. FISH analysis using the Dasypyrum repetitive sequence pDb12H as a probe demonstrated that D11-5 contained a pair of D. breviaristatum chromosomes, while FISH with wheat D-genomic repetitive sequences revealed that the chromosome 2D was absent in D11-5. The functional molecular markers confirmed that the introduced D. breviaristatum chromosomes belong to the homoeologous group 2, indicating that D11-5 was a 2V^b (2D) disomic substitution line. Field resistance showed that the introduced D. breviaristatum chromosomes 2V^b were responsible for the stripe rust resistance at the adult plant stage. FISH, C-banding, and PCR-based molecular marker analysis indicated that the chromosome 2V^b of D. breviaristatum was completely different from the chromosome 2V of D. villosum. The identified wheat-D. breviaristatum chromosome substitution line D11-5 may be applied to produce agronomically desirable stripe rust resistance germplasm.

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Mapping a gene conferring resistance toPseudocercosporella herpotrichoideson chromosome 4V ofDasypyrum villosumin a wheat background

Cited by 47 publications

References 14 publications

Cytogenetics of <i>Triticum</i> × <i>Dasypyrum</i> hybrids and derived lines

Cytogenetics of <i>Triticum</i> × <i>Dasypyrum</i> hybrids and derived lines

Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1

A Novel Wheat-<b><i>Dasypyrum breviaristatum </i></b>Substitution Line with Stripe Rust Resistance

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