Mapping a new secalin locus on the rye 1RS arm

Козуб, Н. А.; Motsnyi, I. I.; Созинов, И. А.; Blume, Ya. B.; Sozinov, A. A.

doi:10.3103/s0095452714040021

Cited by 7 publications

(3 citation statements)

References 18 publications

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“…Assignment to the three wheat subgenomes of the gamma-gliadin amplicons by their proximity to the gene sequence from CS in the phylogenetic tree resulted in a total of 7, 13, and 14 amplicons assigned to A-, B- and D-subgenomes respectively. In addition, seven 40k-γ-secalin amplicons were identified, as they are located in the short arm of the 1R chromosome of rye ( Kozub et al., 2014 ), present on the bread wheat genotypes with the 1BL/1RS rye translocation. However, the subgenome assignation of alpha-gliadin amplicons either by searching subgenome-specific motifs around the 33-mer region ( van Herpen et al., 2006 ) or by their similarity to CS reference genes ( Huo et al., 2018b ) had discrepancies.…”

Section: Discussionmentioning

confidence: 99%

“…As shown in previous research, the 40k-γ-secalins present few epitope hits, including the DQ2.5_glia_γ4c between them. In fact, this secalin type has less epitope than the rest of rye prolamins such as ω-secalins or 75k-γ-secalins ( Lexhaller et al., 2019 ), whose genes are present in the 2R chromosome ( Kozub et al., 2014 ). The other 40k-γ-secalins found on these bread wheat genotypes comprised the Gamma_0 ‘type’, only present in wheat containing the rye translocation.…”

Section: Discussionmentioning

confidence: 99%

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Unraveling the celiac disease-related immunogenic complexes in a set of wheat and tritordeum genotypes: implications for low-gluten precision breeding in cereal crops

2023

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The development of low-gluten immunogenic cereal varieties is a suitable way to fight the increment of pathologies associated with the consumption of cereals. Although RNAi and CRISPR/Cas technologies were effective in providing low-gluten wheat, the regulatory framework, particularly in the European Union, is an obstacle to the short- or medium-term implementation of such lines. In the present work, we carried out a high throughput amplicon sequencing of two highly immunogenic complexes of wheat gliadins in a set of bread and durum wheat, and tritordeum genotypes. Bread wheat genotypes harboring the 1BL/1RS translocation were included in the analysis and their amplicons successfully identified. The number of CD epitopes and their abundances were determined in the alpha- and gamma-gliadin amplicons, including 40k-γ-secalin ones. Bread wheat genotypes not containing the 1BL/1RS translocation showed a higher average number of both alpha- and gamma-gliadin epitopes than those containing such translocation. Interestingly, alpha-gliadin amplicons not containing CD epitopes accounted for the highest abundance (around 53%), and the alpha- and gamma-gliadin amplicons with the highest number of epitopes were present in the D-subgenome. The durum wheat and tritordeum genotypes showed the lowest number of alpha- and gamma-gliadin CD epitopes. Our results allow progress in unraveling the immunogenic complexes of alpha- and gamma-gliadins and can contribute to the development of low-immunogenic varieties within precision breeding programs, by crossing or by CRISPR/Cas gene editing.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Unraveling the celiac disease-related immunogenic complexes in a set of wheat and tritordeum genotypes: implications for low-gluten precision breeding in cereal crops

2023

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“…The presence of the 1RS.1BL translocation in the genome of bread wheat has a negative effect on baking quality [ 32 , 33 , 34 ]. This could be explained by the presence of rye genes coding for ω- and 40 K γ-secalins of the gliadin group encoded by the Sec-1 and, presumably, Sec-N genes, respectively, and changing the fractional composition of the protein, reducing the volumetric yield of bread, the sedimentation index and rheological test properties [ 35 , 36 , 37 , 38 ]. Studies of the effect of the 1RS.1AL translocation on quality scores have shown that its presence does not lead to such a dramatic decrease in these scores compared to 1RS.1BL [ 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

Permanent Spreading of 1RS.1AL and 1RS.1BL Translocations in Modern Wheat Breeding

Korobkova

Bespalova

Yanovsky

et al. 2023

Plants

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Wheat-rye translocations 1RS.1BL and 1RS.1AL are used in bread wheat breeding worldwide because a short arm of rye chromosome 1 (1RS) when introgressed into the wheat genome confers resistance to diseases, pests and better performance under drought-stress conditions. However, in durum wheat genotypes, these translocations occur only in experimental lines, although their advantages could enhance the potential of this crop. P.P. Lukyanenko National Grain Centre (NGC) has successfully developed commercially competitive cultivars of bread and durum wheat demanded by many agricultural producers in the South of Russia for decades. Here, 94 accessions of bread and 343 accessions of durum wheat, representing lines and cultivars from collection, competitive variety trials and breeding nursery developed at NGC were screened for 1RS using PCR markers and genomic in situ hybridization. The 1RS.1BL and 1RS.1AL translocations were detected in 38 and 6 bread wheat accessions, respectively. None of the durum wheat accessions showed translocation, despite the fact that some of them had 1RS.1BL donors in their pedigree. The absence of translocations in the studied durum wheat germplasm can be caused by the negative selection of 1RS carriers at different stages of the breeding process due to low quality and difficulties in transferring rye chromatin through wheat gametes.

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Molecular Detection of Resistance to Biotic Stress Conditions in Spring Bread Wheat Cultivars

Karelov

Козуб

Созинов

et al. 2019

Innovative Approaches and Applications for Sustainable Rural Development

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Mapping a new secalin locus on the rye 1RS arm

Cited by 7 publications

References 18 publications

Unraveling the celiac disease-related immunogenic complexes in a set of wheat and tritordeum genotypes: implications for low-gluten precision breeding in cereal crops

Unraveling the celiac disease-related immunogenic complexes in a set of wheat and tritordeum genotypes: implications for low-gluten precision breeding in cereal crops

Permanent Spreading of 1RS.1AL and 1RS.1BL Translocations in Modern Wheat Breeding

Molecular Detection of Resistance to Biotic Stress Conditions in Spring Bread Wheat Cultivars

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