Mapping and Validation of Quantitative Trait Loci Associated with Tiller Production in Rice

Abstract

Abbreviations: LOD, logarithm of the odds ratio; LT-RIL, Lemont ´ TeQing recombinant inbred line; Mbp, mega base pair; MIM, multiple-interval mapping; N1T, node of first tiller on the main culm; QTL, quantitative trait locus; RFLP, restriction fragment length polymorphism; RIL, recombinant inbred line; SLN, seedling leaf number; SSR, simple-sequence repeat; TIL, TeQing-into-Lemont backcross introgression line; TN, tiller number.

Registration of TIL:383.13, TIL:625 and TIL:634, Three Long Grain Tropical Japonica Rice Germplasm Lines Containing Novel Indica Alleles that Increase Tiller Production and Grain Yield

“…Early production of tillers and their leaves is also associated with faster canopy closure, which can increase weed competitiveness (Dingkuhn et al, 1999; Namuco et al, 2009; Mahajan et al, 2014), providing additional incentive to increase the early tillering ability in US cultivars. Toward this aim, a study was conducted to identify and molecularly map the quantitative trait loci (QTLs) responsible for TeQing's early and prolific tillering (Pinson et al, 2015). A total of nine tiller number QTLs (TN‐QTLs, Table 1) were identified in a set of Lemont × TeQing recombinant inbred lines, eight of which (all except qTN12 ) were verified in the TILs (Pinson et al, 2015).…”

“…Toward this aim, a study was conducted to identify and molecularly map the quantitative trait loci (QTLs) responsible for TeQing's early and prolific tillering (Pinson et al, 2015). A total of nine tiller number QTLs (TN‐QTLs, Table 1) were identified in a set of Lemont × TeQing recombinant inbred lines, eight of which (all except qTN12 ) were verified in the TILs (Pinson et al, 2015). TeQing contributed the allele for increased tillering for all loci except qTN1‐2 .…”

“…†QTLs were mapped previously in two Lemont × TeQing progeny mapping populations (Pinson et al, 2015). …”

“…‡Genotypic characterization of the TILs for 178 simple sequence repeat was determined previously (Pinson et al, 2012) and was used in the QTL mapping study (Pinson et al, 2015). Complete marker data can be obtained from the Genetic Stocks‐ Oryza website (http://www.ars.usda.gov/Main/docs.htm?docid=18992&pf=1&cg_id=0).…”

“…The aim of the present study was to identify and release as breeding parents TILs suitable for introducing into long grain tropical japonica breeding programs indica alleles shown to favor early tillering and canopy development, as well as increased panicle number and grain yield in potted and field‐grown plants. Identification and selection for improved germplasm was first based on molecular data indicating the introgression of one or more QTLs affecting seedling tiller number (TN) and timing of tiller production previously identified in a greenhouse study (Pinson et al, 2015). Final selection was based on field observation that confirmed that the TILs possessing QTLs first identified as increasing TN among potted plants also exhibited enhanced production of tillers, canopy, panicles, and grain yield under drill‐seeded, flooded field conditions.…”