Mapping differences in mammalian distributions and diversity using environmental DNA from rivers

Broadhurst, Holly A.; Gregory, Luke M.; Bleakley, Emma K.; Perkins, J. Clint; Lavin, Jenna V.; Bolton, Polly; Browett, Samuel S.; Howe, Claire V.; Singleton, Natalie; Tansley, Darren; Sales, Naiara Guimarães; McDevitt, Allan D.

doi:10.1016/j.scitotenv.2021.149724

Cited by 31 publications

(39 citation statements)

References 59 publications

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“…However, other semi-aquatic carnivores such as the Eurasian otter have proven to be challenging to detect using eDNA, either not being detected at all in sampled areas when presence was known (Sales et al . 2020a) or requiring the screening of a comparatively large number of samples to be detected (Broadhurst et al . 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Water samples were collected using sterile 500 ml water bottles from the bankside at a reachable distance with complete or near-complete submersion of the bottle beneath the surface. Five 500 ml water replicates (Broadhurst et al . 2021) were collected from each site (see description of camera trapping above), within 5-10 m of each other, in close proximity to the camera traps.…”

Section: Methodsmentioning

confidence: 99%

“…(2020a) with PCR positive controls (i.e. DNA extraction from a non-target species that is not locally present, the northern muriqui Brachyteles hypoxanthus from Brazil included; Broadhurst et al . 2021).…”

Section: Methodsmentioning

confidence: 99%

“…Environmental DNA (eDNA)-based monitoring has not previously been tested for European mink, but has been successful in detecting other carnivores and semi-aquatic species (Sales et al . 2020a; Broadhurst et al . 2021).…”

Section: Introductionmentioning

confidence: 99%

“…2021) and therefore has the potential to be an effective additional method to existing monitoring approaches for European mink (Sales et al . 2020a; Broadhurst et al . 2021).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Mink on the brink: Comparing survey methods for detecting a Critically Endangered carnivore, the European minkMustela lutreola

Croose

Hanniffy

Harrington³

et al. 2022

Preprint

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Monitoring rare and elusive species is critical in guiding appropriate conservation management measures. Mammalian carnivores are challenging to monitor directly, due to their generally nocturnal and solitary lifestyle, and relatively large home ranges. The European mink Mustela lutreola is a Critically Endangered, small, semi-aquatic carnivore and is one of the most threatened mammal species in Europe. In northern Spain, the European mink population is monitored regionally using different methods and approaches, making assessment of national population status difficult. There is an urgent need to 1) assess the efficacy of survey methods, and 2) identify a standard monitoring methodology that can be deployed rapidly and inexpensively over large areas of the mink’s range. We deployed four methods – camera trapping, hair tubes, live trapping, and environmental DNA (eDNA) from water samples – to compare the probability of detecting European mink when present at 25 sampling sites within five 10×10km squares, and the economic cost and time required for each method. All four methods successfully detected European mink but the probability of detection varied by method. Camera trapping and hair tubes had the highest probability of detection; however, eDNA and live trapping detected mink in one 10×10km square where the latter two methods did not. For future European mink monitoring programmes, we recommend a combination of at least two methods, and suggest that camera traps or hair tubes are combined with live trapping or eDNA (depending on the scale and aims of the study), to gather critical information on distribution, occupancy and conservation status.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…2021) and therefore has the potential to be an effective additional method to existing monitoring approaches for European mink (Sales et al . 2020a; Broadhurst et al . 2021).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Mink on the brink: Comparing survey methods for detecting a Critically Endangered carnivore, the European minkMustela lutreola

Croose

Hanniffy

Harrington³

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

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BeeDNA: Microfluidic environmental DNA metabarcoding as a tool for connecting plant and pollinator communities

et al. 2022

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Pollinators are declining globally, and this loss can reduce plant reproduction, erode critical ecosystem services and resilience, and drive economic losses. Monitoring pollinator biodiversity trends is essential for adaptive conservation and management, but conventional surveys are often costly, time-consuming, and requires considerable taxonomic expertise. Environmental DNA (eDNA) metabarcoding surveys are booming due to their rapidity, nondestructiveness, and cost efficiency. Microfluidic technology allows multiple primer sets from different markers to be used in eDNA metabarcoding for more comprehensive inventories, minimizing associated primer bias. We evaluated microfluidic eDNA metabarcoding for pollinator community monitoring in both controlled greenhouse and natural field settings. Using a variety of sampling, preservation, and extraction methods, we assessed pollinator communities with a number of markers using microfluidic metabarcoding. In greenhouse experiments, microfluidic eDNA metabarcoding detected the target bumblebee in two of four focal flower species as well as greenhouse insects in all focal flower species. In the field, numerous common regional arthropods, including some directly observed, were detected. Pollinator detection was maximized using whole flower heads preserved in ATL buffer and extracted with a modified Qiagen® DNeasy protocol for amplification with COI primers. eDNA surveillance could enhance pollinator assessment by detecting protected and endangered species and being more applicable to remote, inaccessible locations, whilst reducing survey time, effort, and expense. Microfluidic eDNA metabarcoding requires optimization to address remaining efficacy concerns, but this approach shows potential in revealing complex networks underpinning critical ecosystem functions and services, enabling more accurate assessments of ecosystem resilience.

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Quantifying biodiversity using eDNA from water bodies: General principles and recommendations for sampling designs

Altermatt

Carraro

Antonetti³

et al. 2023

Environmental DNA

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Reliable and comparable estimates of biodiversity are the foundation for understanding ecological systems and informing policy and decision-making, especially in an era of massive anthropogenic impacts on biodiversity. Environmental DNA (eDNA) metabarcoding is at the forefront of technological advances in biodiversity monitoring, and the last few years have seen major progress and solutions to technical challenges from the laboratory to bioinformatics. Water eDNA has been shown to allow the fast and

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Mapping differences in mammalian distributions and diversity using environmental DNA from rivers

Cited by 31 publications

References 59 publications

Mink on the brink: Comparing survey methods for detecting a Critically Endangered carnivore, the European minkMustela lutreola

Mink on the brink: Comparing survey methods for detecting a Critically Endangered carnivore, the European minkMustela lutreola

BeeDNA: Microfluidic environmental DNA metabarcoding as a tool for connecting plant and pollinator communities

Quantifying biodiversity using eDNA from water bodies: General principles and recommendations for sampling designs

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