Mapping heat tolerance QTLs in Triticum durum-Aegilops speltoides backcross introgression lines to enhance thermotolerance in wheat

Abstract

Wheat, a major cereal crop, is the most consumed staple food after rice in India. Frequent episodes of heat waves during the past decade have raised concerns about food security under impending global warming and necessitate the development of heat-tolerant wheat cultivars. Wild relatives of crop plants serve as untapped reservoirs of novel genetic variations. In the present study a mapping population comprising 311 BC2F10 backcross introgression lines (BILs) developed by crossing Triticum durum and heat-tolerant diploid wild wheat relative Aegilops speltoides accession pau3809 was used to map QTLs for terminal heat tolerance. The homozygous BILs were evaluated for heat stress tolerance component traits under an optimum environment (OE) and a heat-stressed environment (HE) for the two cropping seasons. Data on spike length, spikelet number per spike, peduncle length, thousand-grain weight, grains per spike, days to heading, days to maturity, grain filling duration, NDVI at heading, plant height and plot yield were recorded. Genotyping-by-sequencing (GBS) of the BILs was carried out, and 2945 high-quality, polymorphic SNPs were obtained. Thirty QTLs were detected for various heat tolerance component traits on chromosomes 1A, IB, 2A, 2B, 3B, 4B, 5A, 5B, 6A and 6B with phenotypic variance ranging from 5 to 11.5%. Several candidate genes reported to play a role in heat stress responses were identified by browsing the 1.85 Mb physical region flanking the stable QTLs detected under the HE. Identified QTL and linked markers can be employed for genomics-assisted breeding for heat tolerance in wheat.