Mapping of CGRP in the alpaca diencephalon

Coveñas, Rafael; Sánchez, Manuel Lisardo; Mangas, A.; Medina, L.E.; Aguilar, Luís Fuentes; Dı́az-Cabiale, Zaida; Narváez, José Ángel

doi:10.1016/j.jchemneu.2012.07.004

Cited by 9 publications

(28 citation statements)

References 25 publications

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“…These structures are important for memory processing (Aggleton et al, ). CGRP‐immunoreactive fibers have been demonstrated in the MM (Covenas et al, ), and in this study we found mRNA and protein coexpression of CLR and RAMP1 in neurons of the MM. The fibers may release CGRP to act on neurons expressing its receptor in the MM.…”

Section: Discussionsupporting

confidence: 81%

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Localization of CGRP receptor components and receptor binding sites in rhesus monkey brainstem: A detailed study using in situ hybridization, immunofluorescence, and autoradiography

Eftekhari

Gaspar

Roberts

et al. 2015

J of Comparative Neurology

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Functional imaging studies have revealed that certain brainstem areas are activated during migraine attacks. The neuropeptide calcitonin gene-related peptide (CGRP) is associated with activation of the trigeminovascular system and transmission of nociceptive information and plays a key role in migraine pathophysiology. Therefore, to elucidate the role of CGRP, it is critical to identify the regions within the brainstem that process CGRP signaling. In situ hybridization and immunofluorescence were performed to detect mRNA expression and define cellular localization of calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1), respectively. To define CGRP receptor binding sites, in vitro autoradiography was performed with [(3)H]MK-3207 (a CGRP receptor antagonist). CLR and RAMP1 mRNA and protein expression were detected in the pineal gland, medial mammillary nucleus, median eminence, infundibular stem, periaqueductal gray, area postrema, pontine raphe nucleus, gracile nucleus, spinal trigeminal nucleus, and spinal cord. RAMP1 mRNA expression was also detected in the posterior hypothalamic area, trochlear nucleus, dorsal raphe nucleus, medial lemniscus, pontine nuclei, vagus nerve, inferior olive, abducens nucleus, and motor trigeminal nucleus; protein coexpression of CLR and RAMP1 was observed in these areas via immunofluorescence. [(3)H]MK-3207 showed high binding densities concordant with mRNA and protein expression. The present study suggests that several regions in the brainstem may be involved in CGRP signaling. Interestingly, we found receptor expression and antagonist binding in some areas that are not protected by the blood-brain barrier, which suggests that drugs inhibiting CGRP signaling may not be able to penetrate the central nervous system to antagonize receptors in these brain regions.

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Section: Discussionsupporting

confidence: 81%

“…These structures are important for memory processing (Aggleton et al, 2011). CGRP-immunoreactive fibers have been demonstrated in the MM (Covenas et al, 2012), and in this study we found mRNA and protein coexpression of CLR and…”

Section: MMsupporting

confidence: 71%

Localization of CGRP receptor components and receptor binding sites in rhesus monkey brainstem: A detailed study using in situ hybridization, immunofluorescence, and autoradiography

Eftekhari

Gaspar

Roberts

et al. 2015

J of Comparative Neurology

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“…In this latter study, no double‐labelled cell bodies were detectable, since tyrosine hydroxylase and somatostatinergic neuronal populations showed different morphologies and locations in the diencephalic regions. However, immunoreactive fibers containing somatostatin‐28 (1‐12), calcitonin gene‐related peptide, leucine‐enkephalin, adrenocorticotropin hormone, beta‐endorphin (1‐27), alpha‐melanocyte‐stimulating hormone and alpha‐neo‐endorphin have been located in many diencephalic nuclei of the alpaca (Coveñas et al, ; Manso et al, ). This suggests a close anatomical relationship and a possible functional interaction between somatostatin‐28 (1‐12) and the above‐mentioned peptides.…”

Section: Discussionmentioning

confidence: 99%

“…Alpacas are able to live at sea level and at more than 5,000 m altitude, making this animal an interesting species that could reveal the existence of special and unique regulatory mechanisms. During the last eight years several papers regarding the distribution of neuropeptides in the camelid Lama pacos have been published (Coveñas et al, , 2012de Souza et al, 2007de Souza et al, , 2008de Souza et al, , 2014Manso et al, 2014;Marcos et al, 2011Marcos et al, , 2013. Thus, with the use of immunocytochemical techniques the distribution of fibers and cell bodies containing somatostatin-28 (1-12), calcitonin generelated peptide, adrenocorticotropin hormone, alphamelanocyte-stimulating hormone, beta-endorphin (1-27), alpha-neo-endorphin and leucine-enkephalin have been reported in the alpaca diencephalon (Coveñas et al, , 2012Manso et al, 2014;Marcos et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…During the last eight years several papers regarding the distribution of neuropeptides in the camelid Lama pacos have been published (Coveñas et al, , 2012de Souza et al, 2007de Souza et al, , 2008de Souza et al, , 2014Manso et al, 2014;Marcos et al, 2011Marcos et al, , 2013. Thus, with the use of immunocytochemical techniques the distribution of fibers and cell bodies containing somatostatin-28 (1-12), calcitonin generelated peptide, adrenocorticotropin hormone, alphamelanocyte-stimulating hormone, beta-endorphin (1-27), alpha-neo-endorphin and leucine-enkephalin have been reported in the alpaca diencephalon (Coveñas et al, , 2012Manso et al, 2014;Marcos et al, 2013). Regarding the knowledge of the chemical neuroanatomy of neuropeptides in the alpaca brainstem, the distribution of fibers and perikarya containing leucineenkephalin, calcitonin gene-related peptide and neurotensin has been studied (de Souza et al, 2007(de Souza et al, , 2008(de Souza et al, , 2014Marcos et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Mapping of somatostatin‐28 (1‐12) in the alpaca (Lama pacos) brainstem

Souza

Sánchez

Aguilar

et al. 2015

Microscopy Res & Technique

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Using an indirect immunoperoxidase technique, we studied the distribution of cell bodies and fibers containing somatostatin-28 (1-12) in the alpaca brainstem. Immunoreactive fibers were widely distributed throughout the whole brainstem: 34 brainstem nuclei/regions showed a high or a moderate density of these fibers. Perikarya containing the peptide were widely distributed throughout the mesencephalon, pons and medulla oblongata. Cell bodies containing somatostatin-28 (1-12) were observed in the lateral and medial divisions of the marginal nucleus of the brachium conjunctivum, reticular formation (mesencephalon, pons and medulla oblongata), inferior colliculus, periaqueductal gray, superior colliculus, pericentral division of the dorsal tegmental nucleus, interpeduncular nucleus, nucleus of the trapezoid body, vestibular nucleus, motor dorsal nucleus of the vagus, nucleus of the solitary tract, nucleus praepositus hypoglossi, and in the substantia nigra. This widespread distribution indicates that somatostatin-28 (1-12) is involved in multiple physiological actions in the alpaca brainstem.

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Neurochemical characterization of the hypothalamus of the early fetal and newborn alpaca Vicugna pacos

Montelli

Graïc

Ruiz

et al. 2019

The Anatomical Record

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In this study we performed a neurochemical characterization of the hypothalamus in the developing alpaca (Vicugna pacos) with the aim of revealing the distributions of immunoreactive (-ir) cells containing parvalbumin (PV), calbindin (CB), calretinin (CR), the somatostatin (SOM), the enzyme aromatase P450 (P450Arom), the estrogen receptor α (ER-α), and estrogen receptor β (ER-β) in embryonal stages, early fetal age, and in the newborn. This analysis has been carried out on embryos at 20, 30, 45 days, fetuses at 90 days, and newborn alpaca. Our immunohistochemical results revealed no cells-ir throughout the embryonic hypothalami of 20, 30, and 45 days. On the fetal stage of 90 days, SOM-ir cells were observed in the lateral hypothalamus and the ventromedial nuclei of the tuberal region. We checked for the presence of P450Arom-ir cells in the periventricular area and dorsomedial hypothalamic nucleus of the tuberal region. In these fetal stages, no PV-ir, CB-ir, CR-ir or ERs-ir cells were identified. In the newborn, the PV-ir, CB-ir, CR-ir, and SOMir cells were detected in both the anterior and tuberal hypothalamic area. The P450Arom-ir cells the ER-α-ir and ER-β-ir cells were found in the anterior hypothalamus. Our results offer a contribution in the future purpose to obtain a time-expression pattern of the considered markers in alpaca during gestation and represents a foundation for future investigations on the alpaca brain to define the cross talk between PV, CB, CR, P450Arom, SOM, and ERs in the hypothalamus, the strategic region for the control of the reproductive behavior.

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Mapping of CGRP in the alpaca diencephalon

Cited by 9 publications

References 25 publications

Localization of CGRP receptor components and receptor binding sites in rhesus monkey brainstem: A detailed study using in situ hybridization, immunofluorescence, and autoradiography

Localization of CGRP receptor components and receptor binding sites in rhesus monkey brainstem: A detailed study using in situ hybridization, immunofluorescence, and autoradiography

Mapping of somatostatin‐28 (1‐12) in the alpaca (Lama pacos) brainstem

Neurochemical characterization of the hypothalamus of the early fetal and newborn alpaca Vicugna pacos

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