Mapping of Fruit Shape and Color Development Traits in Eggplant(Solanum melongena L.) Based on RAPD and AFLP Markers.

Nunome, Toshiro; Ishiguro, Keizo; Yoshida, Takeshi; Hirai, Masashi

doi:10.1270/jsbbs.51.19

Cited by 100 publications

(81 citation statements)

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“…A total of 179 AFLP polymorphic bands were detected from 64 primer combinations, but only 32 polymorphic markers were obtained from the survey of 459 eggplant SSR, and very few polymorphisms were revealed by RAPD, SRAP, RGA, and SRAP/RGA techniques. These results confirm the low frequency of DNA polymorphism in the eggplant genome observed in other parentage (Nunome et al 2001). In the end, only 91 AFLP, 26 SSR and 2 SRAP markers were genotyped to construct a genetic linkage map of S. melongena.…”

Section: Discussionsupporting

confidence: 87%

“…This region could correspond to a part of the S. aethiopicum Aculeatum group genome introgressed into AG91-25. Despite a relatively low number of markers, the genetic length of the present map is comparable to the estimate provided by Nunome et al (2001) for an intraspecific F 2 population. We demonstrated that AG91-25 resistance to R. solanacearum phylotype I strains is conferred by a major dominant gene, named ERs1.…”

Section: Discussionsupporting

confidence: 54%

“…The low molecular polymorphism present within eggplant germplasm (Nunome et al 2001) has been a major problem for building genetic maps based on intraspecific segregating populations. Amplified fragment length polymorphism (AFLP) is a good technique for genotyping in such cases, because it generates a large number of genome-wide polymorphic markers.…”

Section: Introductionmentioning

confidence: 99%

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Genetic mapping of a major dominant gene for resistance to Ralstonia solanacearum in eggplant

et al. 2012

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Resistance of eggplant against Ralstonia solanacearum phylotype I strains was assessed in a F 6 population of recombinant inbred lines (RILs) derived from a intra-specific cross between S. melongena MM738 (susceptible) and AG91-25 (resistant). Resistance traits were determined as disease score, percentage of wilted plants, and stem-based bacterial colonization index, as assessed in greenhouse experiments conducted in Réunion Island, France. The AG91-25 resistance was highly efficient toward strains CMR134, PSS366 and GMI1000, but only partial toward the highly virulent strain PSS4. The partial resistance found against PSS4 was overcome under high inoculation pressure, with heritability estimates from 0.28 to 0.53, depending on the traits and season. A genetic map was built with 119 AFLP, SSR and SRAP markers positioned on 18 linkage groups (LG), for a total length of 884 cM, and used for quantitative trait loci (QTL) analysis.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

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Genetic mapping of a major dominant gene for resistance to Ralstonia solanacearum in eggplant

et al. 2012

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“…The first such work involved the localization of a QTL for fruit shape on a random amplified polymorphism (RAPD) map for an intraspecific S. melongena F 2 population (derived from a cross between eggplant lines 'EPL1' and 'WCGR112-8') (Nunome et al 1998). The addition of nearly 100 AFLP Ò markers to the map allowed the detection of QTL for several other traits (fruit, stem, and calyx color) (Nunome et al 2001). More recently, Barchi et al (2012) developed a linkage map for their intraspecific F 2 population derived from a cross between two breeding lines, '305E40' and '67/3'.…”

Section: Introductionmentioning

confidence: 99%

QTL hotspots in eggplant (Solanum melongena) detected with a high resolution map and CIM analysis

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Daunay

et al. 2014

Euphytica

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Fifty-eight F 2 individuals derived from an interspecific cross between cultivated eggplant, Solanum melongena, and its wild relative, S. linnaeanum, were phenotyped for 42 plant, leaf, flower, and fruit traits. Composite interval mapping analysis using genotypic data from 736 molecular markers revealed the positions of 71 statistically significant (P B 0.05) quantitative trait loci (QTL) influencing 32 of the morphological traits. Although most QTL were location-specific, QTL governing three traits (leaf lobing, leaf prickles and prickle anthocyanin) were detected in both experimental locations. Analysis of three additional traits (stem prickles, fruit calyx prickles and fruit length) in both locations yielded QTL in similar but non-overlapping map positions. The majority (69 %) of the QTL corresponded closely with those detected in previous analyses of this data set. However the increased resolution of the linkage map combined with advances in QTL mapping permitted more precise localization, such that the average interval length of these QTL was reduced by 93 %. Thirty-one percent of the QTL were novel, suggesting that simple linear regression with a low density linkage map (the method used in previous studies of this population) missed a substantial portion of significant QTL. Hotspots of QTL affecting plant hairiness, prickliness, and pigmentation were identified on chromosomes 3, 6, and 10, respectively, and may reflect the pleiotropic activity of single structural or regulatory genes at these positions. Based on synteny between the eggplant, tomato, potato and pepper genomes, putative orthologs were identified for 35 % of the QTL suggesting strong conservation of gene function within the Solanaceae. These results should make itThe localization of QTL for 32 morphological traits on the high-resolution map of the eggplant genome has allowed hotspots and putative orthologs with other solanaceous species to be identified. 123 Euphytica (2014) 197:211-228 DOI 10.1007/s10681-013-1060 easier to target particular loci for map-based cloning and marker-assisted selection studies.

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“…The use of interspecific F 2 and advanced backcross populations has proven to be an effective and efficient way of mapping agronomic and horticultural traits in eggplant's relatives: tomato, potato and pepper (e.g., Bonierbale et al 1994;Tanksley et al 1996;Ben Chaim et al 2001). In addition to providing adequate phenotypic and DNA polymorphism which may be limiting in intraspecific eggplant populations (Nunome et al 2001), interspecific populations can supply the starting material for the introgression of valuable traits from wild species into eggplant cultivars. In the current study, S. linnaeanum was used as the wild parent.…”

Section: Introductionmentioning

confidence: 99%

QTL analysis of morphological traits in eggplant and implications for conservation of gene function during evolution of solanaceous species

et al. 2003

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An interspecific F 2 population from a cross between cultivated eggplant, Solanum melongena, and its wild relative, S. linnaeanum, was analyzed for quantitative trait loci (QTL) affecting leaf, flower, fruit and plant traits. A total of 58 plants were genotyped for 207 restriction fragment length polymorphism (RFLP) markers and phenotyped for 18 characters. One to eight loci were detected for each trait with a total of 63 QTL identified. Overall, 46% of the QTL had allelic effects that were the reverse of those predicted from the parental phenotypes. Wild alleles that were agronomically superior to the cultivated alleles were identified for 42% of the QTL identified for flowering time, flower and fruit number, fruit set, calyx size and fruit glossiness. Comparison of the map positions of eggplant loci with those for similar traits in tomato, potato and pepper revealed that 12 of the QTL have putative orthologs in at least one of these other species and that putative orthology was most often observed between eggplant and tomato. Traits showing potential orthology were: leaf length, shape and lobing; days to flowering; number of flowers per inflorescence; plant height and apex, leaf and stem hairiness. The functionally conserved loci included a major leaf lobing QTL (llob6.1) that is putatively orthologous to the potato leaf (c) and/or Petroselinum (Pts) mutants of tomato, two flowering time QTL (dtf1.1, dtf2.1) that also have putative counterparts in tomato and four QTL for trichomes that have potential orthologs in tomato and potato. These results support the mounting evidence of conservation of gene function during the evolution of eggplant and its relatives from their last common ancestor and indicate that this conservation was not limited to domestication traits.

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Mapping of Fruit Shape and Color Development Traits in Eggplant(Solanum melongena L.) Based on RAPD and AFLP Markers.

Cited by 100 publications

References 29 publications

Genetic mapping of a major dominant gene for resistance to Ralstonia solanacearum in eggplant

Genetic mapping of a major dominant gene for resistance to Ralstonia solanacearum in eggplant

QTL hotspots in eggplant (Solanum melongena) detected with a high resolution map and CIM analysis

QTL analysis of morphological traits in eggplant and implications for conservation of gene function during evolution of solanaceous species

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