Mapping of putative quantitative trait loci controlling the total oligosaccharide and sucrose content of Glycine max seeds

Kim, Hyeun Kyeung; Kang, Sung Taeg; Oh, Ki Won

doi:10.1007/s10265-006-0004-9

Cited by 40 publications

(47 citation statements)

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“…In comparison with the reference 'Williams 82' physical map, these two QTL regions were within a similar proximity, with sucrose QTL in the F 3derived population being narrowed down to a smaller region in the SNP analysis (Table 8). In other studies, sucrose QTL were mapped by Kim et al (2005Kim et al ( , 2006 using data from only one location. In a previous study, a sucrose QTL flanked by Satt287 (at 1,631,658-1,631,885 bp of Gm16 in the Williams 82 physical map) and Sct_065 (at 10,498,889-10,499,048 bp of Gm 16, Williams 82) on chromosome 16 was identified by Kim et al (2006); this previously reported QTL was distantly located upstream from the QTL identified in this study.…”

Section: Discussionmentioning

confidence: 99%

“…The major sucrose QTL on chromosome 19 was flanked by SSR markers Satt278 and Satt523, which explained 21.4% of the total phenotypic variation for sucrose content, while the other two sucrose QTLs on chromosome 2 and 11 conferred minor effects. Using another RIL population derived from the cross of 'Keunolkong' ´ 'Shinpaldalkong', Kim et al (2006) found two QTLs located on chromosomes 12 and 16 significantly associated with sucrose content; however, these QTLs explained a relatively low amount of phenotypic variation (<10%) for sucrose content.…”

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Identification of Quantitative Trait Loci for Sucrose Content in Soybean Seed

et al. 2014

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RESEARCHS oybean [Glycine max (L.) Merr.] is a major source of world protein meal and vegetable oil (Soy Stats, 2012). Because of its nutritional and health benefits, soybean is also processed into a variety of fermented and non-fermented food products. Fermented food products include natto, soy sauce, and miso; non-fermented soyfood products include tofu, kori-tofu, yuba, nimame, soymilk, edamame, bean sprouts, and kinako.Soybean seed contains, on average, 40% protein, 20% oil, 35% carbohydrates and 5% ash (Krober and Cartter, 1962); up to 47% of the carbohydrates are soluble sugars (Hymowitz and Collins, 1974). The major soluble sugars are glucose, fructose, disaccharide sucrose, and raffinose family oligosaccharides (RFOs) including raffinose and stachyose (Hymowitz and Collins, 1974;Liu, 1997). Among these soluble sugars, sucrose is the most abundant, which is 25 to 82 g kg -1 , followed by stachyose (14-41 g kg -1 ) and raffinose ABSTRACT Sucrose is a desirable sugar in soybean seed that affects the quality of soyfoods and feed; therefore, soybean cultivars with high sucrose would be valuable for soyfood and soybean meal markets. The objective of this study was to identify quantitative trait loci (QTL) associated with seed sucrose content using simple sequence repeat (SSr) and single nucleotide polymorphism (SNp) markers that can be used for indirect selection in breeding. A low sucrose line, MFS-553, was crossed with a high sucrose plant introduction, pI 243545, to develop an F 2 -derived QTL mapping population. A total of 626 SSr primers covering 20 soybean chromosomes were used to screen the parents and 209 SSr markers were polymorphic. The polymorphic SSr markers were used to genotype 220 F 2:3 lines. Subsequently, a total of 94 F 3:4 lines derived from the initial F 2:3 population were genotyped with 5361 SNp markers spanning 20 chromosomes, of which 2016 were polymorphic. Seed from the F 2:3 , F 3:5 , and F 3:6 lines were analyzed for sucrose using high performance liquid chromatography (HpLC). Following the linkage map construction, composite interval mapping (CIM) and multiple interval mapping (MIM) were performed to locate sucrose QTL. Three novel QTLs for seed sucrose were mapped to chromosomes 5, 9, and 16, accounting for 46, 10 and 8%, respectively, of the phenotypic variation for sucrose content. SSr and SNp markers linked to these QTLs can be used for marker assisted selection (MAS) in breeding soybean lines with the desired sugar profile.

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Section: Discussionmentioning

confidence: 99%

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confidence: 95%

Identification of Quantitative Trait Loci for Sucrose Content in Soybean Seed

et al. 2014

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“…The major QTL, flanked by SSR markers Satt278 and Satt523, located on chromosome 19 explained 21.4% of the total phenotypic variation in sucrose content, while the other two QTL on chromosome 2 and 11 conferred minor effects, respectively. Kim et al (2006) also reported two new QTL, located on chromosomes 12 and 16. These explained a relatively low amount of phenotypic variation (< 10%) in sucrose content.…”

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confidence: 91%

Quantitative trait loci analysis of soluble sugar contents in soybean

2014

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The soluble sugar content in soybean seeds, mainly sucrose, stachyose, raffinose and trace amounts of glucose and fructose, is important for the increasing global market demand for various soyfoods including tofu, soymilk, natto, bean sprouts and edamame due to their nutritional value and health benefits. The objective of this study was to conduct quantitative trait loci (QTL) analysis and identify molecular markers for soluble sugar content in soybean seeds for marker-assisted selection (MAS) in soybean breeding. The content of the five previously mentioned sugars were measured and associated QTLs were mapped based on a F 2 population that was derived from a cross between V97-3000 and V99-5089. Eleven QTLs were detected for the five sugar contents: one for glucose, three each for fructose and sucrose, and two each for raffinose and stachyose. However, only one QTL for sucrose, one QTL for raffinose, and two QTLs for stachyose were identified with LOD > 3.0 and R 2 > 10% from this research. The QTL on chromosome 11 [linkage group (LG) B1] was identified as associated with sucrose, raffinose and stachyose in the same region as previously reported for sucrose and stachyose. The SSR marker, Satt359, on the QTL B1 region had an significant association with sucrose (LOD = 5.192; R 2 = 0.134), raffinose (LOD = 3.95; R 2 = 0.104), and stachyose (LOD = 13.572; R 2 = 0.314); therefore it can be used to assist breeding selection for sucrose, raffinose and stachyose contents simultaneously.

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“…The QTL analysis in soybean located four QTL for oligosaccharide content. Total oligosaccharide and sucrose content in soybean has two common QTL in two linkage groups (LGs) (Kim et al 2006). The total amount of RFOs in Medicago truncatula was similar to that in pea and appears in a rank order system between the parents of the first mapping population LR1 (71.60 versus 75.16 μg/mg) and the parents of the second population LR4 (67.06 versus 59.98 μg/mg).…”

Section: Resultsmentioning

confidence: 88%

“…Maughan et al (2000) identified genomic regions significantly associated with quantitative trait loci (QTL) controlling sucrose content in soybean. In soybean four QTL for oligosaccharide content were identified (Kim et al 2006). In Medicago truncatula QTL mapping revealed one QTL that explained variation in the stachyose/verbascose ratio co-located with a stachyose synthase gene, determining the oligosaccharide composition (Vandecasteele et al 2011).…”

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Variability in the oligosaccharide concentration in seeds of the mapping population of pea (Pisum sativum L.)

Gawłowska

Lahuta²,

Świecicki

et al. 2014

Czech J. Genet. Plant Breed.

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Gawłowska M., Lahuta L., Święcicki W., Krajewski P. (2014): Variability in the oligosaccharide concentration in seeds of the mapping population of pea (Pisum sativum L.). Czech J. Genet. Plant Breed., 50: 157-162.Anti-nutritional compounds are among the obstacles to the use of pea seeds as a protein source in both feed and food. These compounds are poorly digested by both monogastric animals and humans. There are three main oligosaccharides in pea: raffinose, stachyose and verbascose (raffinose family oligosaccharides -RFOs). The concentration of oligosaccharides in dry seeds, the oligosaccharide percent to the total content of soluble sugars and quantitative trait loci (QTLs) were analysed in the mapping population Wt10245 × Wt11238. The composition and concentration of soluble carbohydrates in seeds harvested from two field experiments (2002 and 2004) were analysed by the high resolution gas chromatography method. The Wt10245 × Wt11238 population was chosen because of the greater difference in the concentration of RFOs in seeds between parental lines (56.48 mg/g seed in Wt10245 and 99.1 mg/g seed in Wt11238). The average levels of oligosaccharides (mg/g seed) from both field experiments in the mapping population were: myo-inositol 1.5, sucrose 33.3, galactinol 0.8, raffinose 9.6, stachyose 30.1, verbascose 37.1. The total oligosaccharide concentration was 76.8 mg/g seed. This comprised anaverage of 68% soluble sugars, with the range from 59% to 75%. The results are in agreement with the knowledge of RFO biosynthesis. This makes selection for changes in the proportion of the particular oligosaccharides difficult, like in Phaseolus. However, it is possible to decrease the RFO content in pea seeds. The linkage between QTL and the gene r is interesting. The rugosus (r) locus changes the morphology and distribution of starch grains, decreases the total starch accumulation, produces a higher ratio of amylose to amylopectin and higher sugar and water content during development along with changes in cell size and lipid content.Keywords: genetic map; molecular markers; QTL; rugosus (r) locus; seed oligosaccharide Pea seeds are an important source of protein. However, their value as ingredients in food and feed is limited by a considerable amount of anti-nutritional factors, among them sucrose α-galactosides (raffinose, stachyose and verbascose, named the raffinose family of oligosaccharides, RFOs). Because of the lack of α-galactosidase in the human digestive system, RFOs appear unchanged in the large intestine, where they are degraded and fermented by microflora, causing cramps and flatulence (Coon et al. 1990). In effect, the true metabolizable energy and digestibility can be decreased. However, the presence of RFOs in the digestive tract stimulates development of some probiotic bacteria. The nutritional importance of

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Mapping of putative quantitative trait loci controlling the total oligosaccharide and sucrose content of Glycine max seeds

Cited by 40 publications

References 14 publications

Identification of Quantitative Trait Loci for Sucrose Content in Soybean Seed

Identification of Quantitative Trait Loci for Sucrose Content in Soybean Seed

Quantitative trait loci analysis of soluble sugar contents in soybean

Variability in the oligosaccharide concentration in seeds of the mapping population of pea (Pisum sativum L.)

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