Mapping of qualitative and quantitative phenotypic traits in Rosa using AFLP markers

Abstract

A segregating population of 91 hybrids issued from a cross between a dihaploid rose, derived from the haploidisation of a modern cultivar, and a diploid species was used to construct linkage maps of the parental genomes. As in other recent genetic studies in Rosa, AFLPs were used as molecular markers. Two segregating qualitative traits, recurrent blooming and double corolla, already known to be inherited as single recessive and dominant genes, respectively, were recorded in the mapping population. A quantitative trait, thorn density of the shoots, was also evaluated in this population. Sixty eight and 108 AFLP markers located on 8 and 6 linkage groups could be analysed in the female and male parent, respectively. The two recorded qualitative phenotypic markers were mapped as well as the quantitative one, after having performed QTL analyses on the parental maps in the latter case. It appears that thorn quantity is controlled by a major and a minor QTL which are located on the same linkage group at 36.5 and 3.2 cM from the single seasonal-blooming gene, respectively.

Genetic linkage maps of rose constructed with new microsatellite markers and locating QTL controlling flowering traits

“…The F 1 mapping population used in this study has been previously used to develop an AFLP genetic map (Crespel et al 2002). This interspecific population of 91 diploids was generated from a cross between dihaploid rose, H190, obtained from haploidisation of tetraploid Rosa hybrida cv Zambra (Meynet et al 1994) and a hybrid of diploid R. wichuraiana, Rw, originating from Jardin de Bagatelle (Paris, France).…”

“…These include flower color and double corolla (Debener and Mattiesch 1999), prickles on the petiole (Rajapakse et al 2001), single seasonal blooming and double corolla (Crespel et al 2002), black spot resistance (Linde et al 2004;Malek et al 2000), and quantitative traits prickles on the stem (Crespel et al 2002), leaf size, days to flowering and resistance to powdery mildew (Dugo et al 2005).…”

“…Genetic maps have been previously constructed in diploid (Debener 1999;Debener and Mattiesch 1999;Crespel et al 2002;Dugo et al 2005) and tetraploid (Rajapakse et al 2001) roses. These maps were constructed with dominant amplified fragment-length polymorphism (AFLP) and randomly amplified polymorphic DNA markers.…”

“…Latter SSRs represent the first report of SSR isolated from EST in rose. Both kinds of SSRs were used to expand the AFLP genetic map previously reported (Crespel et al 2002) and to obtain a comprehensive genetic linkage map of the diploid rose genome. Using codominant SSR markers, we were able to identify homologous linkage groups (LGs) between the two parental maps.…”

Synteny in the Rosaceae

“…These maps were constructed almost entirely with dominant markers (RAPDs and AFLPs), which implies that they cannot be compared or used for synteny analysis with other members of the Rosaceae. Two of these maps were obtained in diploid F 1 populations (Debener and Mattiesch 1999;Crespel et al 2002) and one in an F 2 between two tetraploid genotypes (Rajapakse et al 2001). …”