Mapping of Quantitative Trait Loci Controlling Resistance ofBrassica oleraceatoXanthomonas campestrispv.campestrisin the Field and Greenhouse

Camargo, L. E. A.; Williams, Paul H.; Osborn, T. C.

doi:10.1094/phyto-85-1296

Cited by 72 publications

(53 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previous studies identified four Xcc resistance QTLs [17] and self-incompatibility locus [33] in the cabbage BI-16× broccoli OSU Cr-7 mapping population, of which linkage map was constructed using WG, TG, and EC RFLP markers. Those markers were renamed as pW and pX according to the NCBI DNA data base.…”

Section: Alignment Of Different Mapsmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of Positions of QTLs Conferring Resistance to Xanthomonas campestris pv. campestris in Brassica oleracea

Tonu¹,

Doullah²,

Shimizu³

et al. 2013

AJPS

View full text Add to dashboard Cite

Black rot, caused by Xanthomonas campestris pv. campestris (Xcc) is possibly the most important disease of Brassica worldwide. To compare chromosomal positions of Xcc resistance loci in Brassica oleracea between the present and published studies and to develop marker assisted selection (MAS) to resistance against Xcc race 1, we constructed a B. oleracea map, including pW, pX and BoCL markers that were closely linked to previously reported Xcc resistance QTLs. We also analyzed Xcc resistance QTLs by improving our previously reported map derived from the cross of a susceptible double-haploid line (GC P09) with a resistant double-haploid line (Reiho P01). In the nine linkage groups obtained (C1-C9), the major QTL, XccBo(Reiho)2, was derived from Reiho with a maximum LOD score (7.7) in C8. The QTL (LOD 4.4) located in C9, XccBo(GC)1 was derived from the susceptible GC. The other QTL (LOD 4.4), XccBo(Reiho)1, was found in C5. Based on common markers, it was possible to compare our finding Xcc resistance QTLs with the B. oleraceaXcc loci reported by previous authors; XccBo(Reiho)1 and XccBo(GC)1 may be identical to the Xcc resistance QTLs reported previously or a different member contained in the same resistance gene cluster. Our map includes public SSR markers linked to Xcc resistance genes that will promote pyramiding Xcc resistance genes in B. oleracea. The present study will also contribute to a better understanding of genetic control of Xcc resistance.

show abstract

Section: Alignment Of Different Mapsmentioning

confidence: 99%

“…[15] also reported that the resistance of BI-16 to race 1 was quantitative and recessive. [17] identified the two Xcc resistance QTLs on LG2 and another two QTLs on LG1 and LG9, respectively, in the mapping population derived from the cross of the resistant cabbage and the susceptible broccoli. [18] also identified QTLs on C2, C4, and C5.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Positions of QTLs Conferring Resistance to Xanthomonas campestris pv. campestris in Brassica oleracea

Tonu¹,

Doullah²,

Shimizu³

et al. 2013

AJPS

View full text Add to dashboard Cite

show abstract

“…This RFLP was used as a co-dominant marker in an F 2 population (91 individuals) and segregated to resistance to Xcc. A linkage analysis of the resistance phenotypes of F 2 plants and their marker genotypes indicated no significant association (F = 0.21, p < 0.01) between the marker and resistance QTLs previously mapped in this population (Malvas et al, 1999;Camargo et al, 1995).…”

Section: Resultsmentioning

confidence: 61%

“…The cabbage inbred line Badger Inbred-16 (BI-16), resistant to Xcc (Camargo et al, 1995) and the susceptiblè fast cycling' inbred line Lc201 (Williams and Hill, 1986) were used in this study. An F 2 population derived from the cross BI-16 x Lc201 previously screened for black rot resistance (Malvas et al, 1999) was used in a segregation analysis to study the linkage relationships between disease resistance homologs and quantitative trait loci (QTL) controlling resistance to X. campestris pv.campestris.…”

Section: Plant Materials and Inoculation Proceduresmentioning

confidence: 99%

See 1 more Smart Citation

A homolog of the RPS2 disease resistance gene is constitutively expressed in Brassica oleracea

et al. 2003

View full text Add to dashboard Cite

In this study, we identified disease resistance gene homologs in Brassica oleracea and assessed their expression in lines resistant and susceptible to Xanthomonas campestris pv. campestris (Xcc). Two DNA fragments of approximately 2.5 kb (BI-16/RPS2 and Lc201/RPS2) were amplified by PCR from two Brassica lines using primers based on an RPS2 homologous sequence previously described in the Brassica oleracea ecotype B117. The sequences of these fragments shared high similarity (95-98%) with RPS2 homologs from various Brassica species. The digestion of these fragments with restriction enzymes revealed polymorphisms at the Xba I restriction sites. The length polymorphisms were used as a co-dominant marker in an F 2 population developed to segregate for resistance to Xcc, the causal agent of black rot. Linkage analysis showed no significant association between the marker and quantitative trait loci for black rot. RT-PCR with specific primers yielded an expected 453 bp fragment that corresponded to the RPS2 homologs in both resistant and susceptible lines inoculated with the pathogen, as well as in non-inoculated control plants. These results suggest that these homologs are constitutively expressed in B. oleracea.

show abstract

Temporal and spatial assessment of defense responses in resistant and susceptible cabbage varieties during infection with Xanthomonas campestris pv. campestris

Gay

Tuzun

2000

Physiological and Molecular Plant Pathology

View full text Add to dashboard Cite

Mapping of Quantitative Trait Loci Controlling Resistance ofBrassica oleraceatoXanthomonas campestrispv.campestrisin the Field and Greenhouse

Cited by 72 publications

References 0 publications

Comparison of Positions of QTLs Conferring Resistance to <i>Xanthomonas campestris</i> pv. <i>campestris</i> in <i>Brassica oleracea</i>

Comparison of Positions of QTLs Conferring Resistance to <i>Xanthomonas campestris</i> pv. <i>campestris</i> in <i>Brassica oleracea</i>

A homolog of the RPS2 disease resistance gene is constitutively expressed in Brassica oleracea

Temporal and spatial assessment of defense responses in resistant and susceptible cabbage varieties during infection with Xanthomonas campestris pv. campestris

Contact Info

Product

Resources

About

Mapping of Quantitative Trait Loci Controlling Resistance ofBrassica oleraceatoXanthomonas campestrispv.campestrisin the Field and Greenhouse

Cited by 72 publications

References 0 publications

Comparison of Positions of QTLs Conferring Resistance to &lt;i&gt;Xanthomonas campestris&lt;/i&gt; pv. &lt;i&gt;campestris&lt;/i&gt; in &lt;i&gt;Brassica oleracea&lt;/i&gt;

Comparison of Positions of QTLs Conferring Resistance to &lt;i&gt;Xanthomonas campestris&lt;/i&gt; pv. &lt;i&gt;campestris&lt;/i&gt; in &lt;i&gt;Brassica oleracea&lt;/i&gt;

A homolog of the RPS2 disease resistance gene is constitutively expressed in Brassica oleracea

Temporal and spatial assessment of defense responses in resistant and susceptible cabbage varieties during infection with Xanthomonas campestris pv. campestris

Contact Info

Product

Resources

About

Comparison of Positions of QTLs Conferring Resistance to <i>Xanthomonas campestris</i> pv. <i>campestris</i> in <i>Brassica oleracea</i>

Comparison of Positions of QTLs Conferring Resistance to <i>Xanthomonas campestris</i> pv. <i>campestris</i> in <i>Brassica oleracea</i>