Mapping of seed quality traits in the C genome of <i>Brassica napus</i> by using a population carrying genome content of <i>B. oleracea</i> and their effect on other traits

Rahman, Habibur; Kebede, Berisso

doi:10.1002/tpg2.20078

Cited by 5 publications

(5 citation statements)

References 68 publications

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“…The SNPs of the C5 QTL that we detected in the present study is located at about 45 Mb position; therefore, the C5 QTL that we detected in the present study could be the same as the one reported by Behnke et al. (2018) and Rahman and Kebede (2021) (Figure 4).…”

Section: Discussionsupporting

confidence: 89%

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Identification of QTL and alleles for agronomic and seed quality traits in C genome using a Brassica napus population diversified with B. oleracea

et al. 2022

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A genome-wide association study (GWAS) was carried out by using a Brassica napus population of 175 lines, developed from six B. napus × B. oleracea interspecific crosses, and 5,743 single nucleotide polymorphism (SNP) markers to identify quantitative trait loci (QTL) for agronomic and seed quality traits and to understand the effect of B. oleracea alleles on these traits. Heritability of these traits varied from 52.9 to 84.1%. About 79% of the SNPs were positioned to the nine C genome chromosomes, while only 21% to the 10 A genome chromosomes; this was largely due to little genetic variation in the A genome of this population, as expected. However, the SNPs were distributed throughout the entire length of the chromosomes suggesting their usefulness in GWAS. The C genome SNPs detected nine genomic regions affecting these traits. This included the genomic regions of C2 and C5 affecting days to flowering, C1 affecting the duration of grain-filling period, C1, C5, and C8 affecting oil content, and C1, C2, and C6 affecting glucosinolate content; among these, some of the loci has not been reported previously. The QTL alleles of B. oleracea which can be beneficial in oilseed B. napus, such as the C5 QTL allele for the earliness of flowering, were also identified. Several putative candidate genes were identified in the QTL regions. Thus, the results provided evidence of the utility of the B. oleracea gene pool for use in unveiling the unidentified QTL in B. napus as well as its use in breeding. INTRODUCTIONBrassica napus canola (AACC, 2n = 38) is the second largest oilseed crop in the world after soybean [Glycine max (L.) Merr.]. The current annual production of Brassica oilseeds in the world is about 71 million metric tonnes (Statista, 2019

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Section: Discussionsupporting

confidence: 89%

“…(2019) at 5.89 Mb, Pal et al. (2021) at 18.65 Mb, and Rahman and Kebede (2021) at 40.0–41.7 Mb positions. The SNPs of the C5 QTL that we detected in the present study is located at about 45 Mb position; therefore, the C5 QTL that we detected in the present study could be the same as the one reported by Behnke et al.…”

Section: Discussionmentioning

confidence: 91%

Identification of QTL and alleles for agronomic and seed quality traits in C genome using a Brassica napus population diversified with B. oleracea

et al. 2022

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“…The details of the field trials for agronomic and seed quality traits of the DH population can be found in Rahman et al (2017) and Rahman and Kebede (2021), for the growth chamber experiments for photosensitivity in Rahman et al (2018), and for root and aboveground biomass in Kebede and Rahman (2019).…”

Section: Plant Materialsmentioning

confidence: 99%

“…Genotypic data of the 88 DH lines obtained by using 113 SSR (Rahman et al 2017(Rahman et al , 2021 (Nikzad et al 2019) and 8,091 SNP markers were used to estimate genetic similarity between all possible pairs of lines of these two populations. The SNP data of the inbred population was obtained by using targeted genotyping by sequencing (tGBS) technique (Nikzad 2020).…”

Section: Genetic Similarity/differencementioning

confidence: 99%

Analysis of single nucleotide polymorphism and simple sequence repeat markers for the estimates of genetic diversity by using two oilseed Brassica napus populations carrying genome contents of Brassica oleracea

2022

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Reliable estimates of genetic diversity (GD) among the accessions in a breeding population is important for use of this knowledge in breeding. Among the different types of molecular markers, SNP and SSR are largely used by breeders; however, our knowledge of the reliability of the estimates of GD based on these two types of markers in multiple populations is limited. In this study, a doubled haploid (DH) and an inbred population developed from Brassica napus × Brassica oleracea interspecific crosses were used for comparative analysis of these two types of markers. The estimates based on SNP and SSR markers showed a stronger correlation in the inbred population which was expected to carry greater GD as compared to the DH population. This inference was also evident from the analysis of different diversity groups (least, intermediate and most similar) of these two populations for significant difference between the groups for six agronomic and seed quality traits, where this analysis failed to differentiate the diversity groups of the DH population for any of the traits. However, both marker types could differentiate the diversity groups of the inbred population for several traits. Furthermore, the six sub-populations of the inbred population could also be differentiated by both marker types. Thus, the results demonstrate the greater utility of the SSR and SNP markers in a genetically diverse population. This knowledge can be used while grouping a breeding population for diversity groups; however, caution needs to be taken while using the markers in a genetically narrow population.

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“…Brassica napus ( B. napus ) is a worldwide oil crop, which is one of the important edible oils for human consumption and as a raw material for the biofuel and pharmaceutical industry [ 23 – 25 ]. Therefore, increasing the oil content of seeds is very important for geneticists and breeders, and has become a major subject of oil crop research [ 26 – 28 ]. In B. napus , LACSs play pivotal roles in lipid biosynthesis and oil accumulation [ 7 , 29 – 31 ].…”

Section: Introductionmentioning

confidence: 99%

Overexpression the BnLACS9 could increase the chlorophyll and oil content in Brassica napus

Zhu

Zheng

et al. 2023

Biotechnol Biofuels

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Background Chlorophyll is a very important pigment involved in photosynthesis, while plant acyl-CoA biosynthesis is derived from plastid-localized fatty acids (FAs). Until now, the regulation of the acyl-CoA pathway for chlorophyll biosynthesis is still unknown. Results Here, we identified a long-chain acyl-CoA synthetase (LACS) gene BnLACS9 from Brassica napus. BnLACS9 complemented a LACS-deficient yeast strain YB525, which indicated that BnLACS9 has the LACS function. BnLACS9 was localized in the chloroplast envelope membrane, while mainly expressed in young leaves and flowers. Overexpression of BnLACS9 in Nicotiana benthamiana resulted in an increase in total CoA and MGDG content. In B. napus with overexpression of BnLACS9, the number of chloroplast grana lamellae and the chlorophyll content, as well as the MGDG and DGDG contents, increased compared to wild type. The net photosynthetic rate, dry weight of the entire plant and oil content of seeds increased significantly, accompanied by an increase in chlorophyll content. Transcriptome analysis revealed that overexpression of BnLACS9 improved the pathway of acyl-CoA biosynthesis and further improved the enzymes in the glycolipid synthesis pathway, while acyl-CoA was the substrate for glycolipid synthesis. The increased glycolipids, especially MGDG and DGDG, accelerated the formation of the chloroplast grana lamellae, which increased the number of chloroplast thylakoid grana lamella and further lead to increased chlorophyll content. Conclusions In the present study, we demonstrated that BnLACS9 played a crucial role in glycolipids and chlorophyll biosynthesis in B. napus. The results also provide a new direction and theoretical basis for the improvement of the agronomic traits of plants.

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Mapping of seed quality traits in the C genome of Brassica napus by using a population carrying genome content of B. oleracea and their effect on other traits

Cited by 5 publications

References 68 publications

Identification of QTL and alleles for agronomic and seed quality traits in C genome using a Brassica napus population diversified with B. oleracea

Identification of QTL and alleles for agronomic and seed quality traits in C genome using a Brassica napus population diversified with B. oleracea

Analysis of single nucleotide polymorphism and simple sequence repeat markers for the estimates of genetic diversity by using two oilseed Brassica napus populations carrying genome contents of Brassica oleracea

Overexpression the BnLACS9 could increase the chlorophyll and oil content in Brassica napus

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