Mapping of two genetic loci, Ten-1 and Ten-2, associated with thymus enlargement in BUF/Mna rats

Murakumo, Yoshiki; Takahashi, Masahide; Sharma, Nidhi; Arakawa, Atsushi; Saito, Mitsuhiro; Amo, Hiroyuki; Matsuyama, Mutsushi

doi:10.1007/s003359900152

Cited by 16 publications

(11 citation statements)

References 23 publications

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“…Of particular interest, BUF/Mna rats carry germ-line mutations predisposing the animals to spontaneous thymoma formation at 9 ± 12 months of age (Matsuyama et al, 1988). Genetic back-cross experiments have identi®ed two loci, Ten-1 (on chromosome 1) and Ten-2 (on chromosome 13) which appear to contribute to thymus enlargement (Murakumo et al, 1996). Thymoma formation in these animals also requires the presence of Tsr-1 (located on chromosome 1, see Murakumo et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

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Heritable lympho-epithelial thymoma resulting from a transgene insertional mutation

Nakajima

Soonpaa

et al. 2000

Oncogene

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Thymoma is the most common tumor of the anteriorsuperior mediastinum. We have identi®ed a line of transgenic mice which spontaneously and heritably develop thymomas at a very high penetrance. The available data suggest that thymoma formation in these mice results as a consequence of transgene insertional mutagenesis. Immune histologic analyses indicate that the thymomas are of epithelial cell origin. Survival studies indicate that tumor progression is more aggressive in females as compared to males (73.9 vs 41.7% mortality at 20 weeks of age, respectively). Fluorescent in situ hybridizations have localized the transgene integration site to the F2-G region of mouse chromosome 2. Translocation encompassing the syntenic region in humans has been implicated in lympho-epithelial thymoma. These animals may constitute a useful resource for the identi®cation of gene(s) which participate in thymoma progression, as well as a model system for screening anti-thymoma therapeutic agents. Oncogene (2000) 19, 32 ± 38.

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Section: Discussionmentioning

confidence: 99%

“…Thymoma has also been reported to occur spontaneously in BUF/Mna rats (Matsuyama et al, 1988). Tumor progression in these animals appears to require the activities of three loci, designated Ten-1, Ten-2 and Tsr-1 (Murakumo et al, 1993(Murakumo et al, , 1996.…”

Section: Introductionmentioning

confidence: 99%

Heritable lympho-epithelial thymoma resulting from a transgene insertional mutation

Nakajima

Soonpaa

et al. 2000

Oncogene

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“…7 (Yamada et al, 1994;Serikawa et al, 1998). Rats of the BUF/Mna strain developed hyperplasia of the thymus in the early life, as an expression of thymus enlargement loci (Ten1 and Ten2) (Murakumo et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Establishment of thymoma-prone congenic rat strain, ACI.BUF/Mna-Tsr1/Tsr1

Matsuyama

Kato

Higo-Moriguchi

et al. 2008

J Cancer Res Clin Oncol

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“…DNA was prepared by serial extractions with phenol/chloroform and ethanol precipitation, as previously reported [9].…”

Section: Animals and Dna Preparationmentioning

confidence: 99%

Generation of a Polymorphic Marker Linked to Thymoma Susceptibility Gene of Rat 1 by Genetically-Directed Representational Difference Analysis.

Higo

Oyabu

et al. 2000

Exp. Anim.

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BUF/Mna (BUF) is a rat strain susceptible to spontaneous development of thymomas. We have previously shown that the thymoma susceptibility is controlled principally by a dominant susceptibility gene located on chromosome 7, thymoma susceptibility gene of rat 1 (Tsr1). To generate genetic markers tightly linked to Tsr1, we performed genetically directed representational difference analysis (GDRDA) with three combinations of the tester and driver DNAs. From 124 {ACI/NMs × (BUF × ACI/NMs) F 1 } backcross rats, 12 rats with the ACI/BUF genotype in the Tsr1 region (A/B rats) and 13 rats with the ACI/ACI genotype in the region (A/A rats) were selected, and their DNAs were pooled, respectively. Three kinds of tester DNAs, i) inbred BUF, ii) (BUF × ACI)F 1 , and iii) the pool from the A/B rats, were subtracted by the driver DNA prepared from the pool of the A/A rats. The three combinations yielded one, two, and one polymorphic marker(s), respectively. One marker, D7Ncc28, was isolated commonly by the three combinations of subtraction, and another marker, D11Ncc12 was isolated only by the second combination. Linkage analysis demonstrated that D7Ncc28 was located in the 8.3 cM region where Tsr1 has been mapped. The three combinations of subtraction were shown to be almost equally capable of isolating polymorphic markers in a specific chromosomal region.

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Mapping of two genetic loci, Ten-1 and Ten-2, associated with thymus enlargement in BUF/Mna rats

Cited by 16 publications

References 23 publications

Heritable lympho-epithelial thymoma resulting from a transgene insertional mutation

Heritable lympho-epithelial thymoma resulting from a transgene insertional mutation

Establishment of thymoma-prone congenic rat strain, ACI.BUF/Mna-Tsr1/Tsr1

Generation of a Polymorphic Marker Linked to Thymoma Susceptibility Gene of Rat 1 by Genetically-Directed Representational Difference Analysis.

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