Mapping Ol-4, a gene conferring resistance to Oidium neolycopersici and originating from Lycopersicon peruvianum LA2172, requires multi-allelic, single-locus markers

Bai, Yuling; Hulst, Ron van der; Huang, Cai-Cheng; Wei, Ling; Stam, P.; Lindhout, P.

doi:10.1007/s00122-004-1698-5

Cited by 34 publications

(21 citation statements)

References 23 publications

(31 reference statements)

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“…Research in the last 15 years has broadened knowledge of the genetic basis of resistance to O. neolycopersici (Lindhout et al 1994b;Ciccarese et al 1998;Huang et al 2000b;Bai et al 2003Bai et al , 2004Bai et al , 2005Li et al 2006Li et al , 2007Li et al , 2008Li et al , 2012Seifi et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Resistance mechanisms of wild tomato germplasm to infection of Oidium neolycopersici

et al. 2013

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Tomato powdery mildew (Oidium neolycopersici) is one of the most devastating diseases of cultivated tomatoes worldwide. Although the first epidemics were recorded more than 25 years ago many aspects of this host-pathogen interaction are still not well understood. Detailed morphological and molecular studies of the anamorphs confirmed that O. neolycopersici is phylogeneticaly close to Erysiphe aquilegiae var. ranunculi. Host range is rather broad, apart from Solanaceae hosts were found in the families Apocynaceae, Campanulaceae, Crassulaceae, Cistaceae, Cucurbitaceae, Linaceae, Malvaceae, Papaveraceae, Pedialiaceae, Scrophulariaceae, Valerianaceae a Violaceae. Non-host resistance within these families is not based on inhibition of formation of primary haustorium, however, on post-haustorial hypersensitive reponse and another type of non-hypersensitive resistance. Screening of wild Solanum species (previous Lycopersicon spp.) germplasm revealed valuable sources of resistance (S. habrochaites, S. pennellii, S. cheesmaniae, S. chilense, S. peruvianum). The main resistance mechanism was found to be a hypersensitive response (HR), in some cases followed by limited development of the pathogen. However, there is a broad variation in resistance response on the histological and cytological level. Interaction between many wild Solanum spp. and O. neolycopersici is race-specific, at least three races were differentiated. In some interspecific crosses (S. lycopersicum × S. habrochaites) adult plant resistance was observed. Biochemical studies focusing on production of reactive oxygen species (ROS) and peroxidase activity during infection of O. neolycopersici showed that production of ROS and activity of corresponding enzymes is related to activation of defence responses in genotypes of wild Solanum sect. Lycopersicon. The significance of nitric oxide (NO) in O. neolycopersici pathogenesis was supported by experiments with NO donors and scavengers. In moderately resistant genotype S. chmielewskii, treatment by heat stress caused slight deceleration of pathogen development, increased production of jasmonic acid (JA) and abscisic acid (ABA) and increased peroxidase activity in infected plants. The different degree of tomato resistance/ susceptibility did not markedly change the rate and extent of photosynthetic response to O. neolycopersici; only minimal impairment of photosynthesis was found in both susceptible and moderately resistant genotypes during the Harper Adams University, Newport, Shropshire TF10 8NB, UK first 9 days after inoculation. The accumulated evidence confirm a crucial role of localised increased production of ROS and reactive nitrogen species (RNS) in response to pathogen penetration into plant tissue and its involvement in the plant resistance responses including the initiation and progression of plant cell death in host wild Solanum species. Crucial points of further research are discussed.

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Section: Introductionmentioning

confidence: 99%

Resistance mechanisms of wild tomato germplasm to infection of Oidium neolycopersici

et al. 2013

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“…In The Netherlands, the Centre for BioSystems Genomics (CBSG) is in charge of the sequencing of tomato chromosome 6. This chromosome contains various genes for economically important traits, including resistance genes for Oidium neolycopersici (Ol-4 and Ol-6), Cladosporium fulvum (Cf-2 and Cf-5), root-knot nematode, aphids and whitefly , and the tomato yellow leaf curl virus (Ty-1, Ty-3, and Ty-4) (van Daelen et al 1993;Weide et al 1993;van Wordragen et al 1994van Wordragen et al , 1996Milligan et al 1998;Ammiraju et al 2003;Bai et al 2004;Seah et al 2004;Ji et al 2007).…”

mentioning

confidence: 99%

Cross-Species Bacterial Artificial Chromosome–Fluorescence in Situ Hybridization Painting of the Tomato and Potato Chromosome 6 Reveals Undescribed Chromosomal Rearrangements

et al. 2008

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Ongoing genomics projects of tomato (Solanum lycopersicum) and potato (S. tuberosum) are providing unique tools for comparative mapping studies in Solanaceae. At the chromosomal level, bacterial artificial chromosomes (BACs) can be positioned on pachytene complements by fluorescence in situ hybridization (FISH) on homeologous chromosomes of related species. Here we present results of such a cross-species multicolor cytogenetic mapping of tomato BACs on potato chromosomes 6 and vice versa. The experiments were performed under low hybridization stringency, while blocking with Cot-100 was essential in suppressing excessive hybridization of repeat signals in both within-species FISH and cross-species FISH of tomato BACs. In the short arm we detected a large paracentric inversion that covers the whole euchromatin part with breakpoints close to the telomeric heterochromatin and at the border of the short arm pericentromere. The long arm BACs revealed no deviation in the colinearity between tomato and potato. Further comparison between tomato cultivars Cherry VFNT and Heinz 1706 revealed colinearity of the tested tomato BACs, whereas one of the six potato clones (RH98-856-18) showed minor putative rearrangements within the inversion. Our results present cross-species multicolor BAC-FISH as a unique tool for comparative genetic studies across Solanum species.

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“…There is some evidence suggesting that Ol-1 and Ol-3 are allelic variants (Huang et al 2000b;Bai et al 2005). Ol-4, originating from S. peruvianum LA2172, is located on the short arm of chromosome 6 (Bai et al 2004). Ol-5, introgressed from S. habrochaites PI247087, is closely linked to Ol-1 and Ol-3 on the long arm of chromosome 6 (Bai et al 2005).…”

Section: Mapped Loci For Resistance To O Neolycopersici In Tomatomentioning

confidence: 99%

Genetics and molecular mechanisms of resistance to powdery mildews in tomato (Solanum lycopersicum) and its wild relatives

et al. 2013

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Powdery mildews (PMs) cause disease in a wide range of plant species including important crops. Taking tomato as an example, here we review findings on the genetic basis and mechanisms of plant resistance to PMs. First, we present a summary of our research on tomato resistance to two PM species, with the focus on Oidium neolycopersici. We discuss the genetics of resistance to this pathogen in tomato. Then, we compare different forms of resistance mediated by different resistance genes based on molecular and cytological data. Also, we provide a comparison between these resistance genes in tomato with those in barley, Arabidopsis and wheat, in order to present a model for the genetic basis of resistance to PMs in plants. We try to accommodate these resistance mechanisms in the current model of plant innate immunity. At the end we discuss possibilities to translate these findings to practical approaches in breeding for resistance to PMs in crops.

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Mapping Ol-4, a gene conferring resistance to Oidium neolycopersici and originating from Lycopersicon peruvianum LA2172, requires multi-allelic, single-locus markers

Cited by 34 publications

References 23 publications

Resistance mechanisms of wild tomato germplasm to infection of Oidium neolycopersici

Resistance mechanisms of wild tomato germplasm to infection of Oidium neolycopersici

Cross-Species Bacterial Artificial Chromosome–Fluorescence in Situ Hybridization Painting of the Tomato and Potato Chromosome 6 Reveals Undescribed Chromosomal Rearrangements

Genetics and molecular mechanisms of resistance to powdery mildews in tomato (Solanum lycopersicum) and its wild relatives

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