Mapping QTLS for Yield and Yield Components Under Drought Stress in Bread Wheat (Triticum Aestivum L.)

Abstract

The aim of this study was to discover the geneic basis of drought tolerance, a double haploid mapping population, Drought Mapping Population 5 (DR. M.P. 5) was assessed for drought tolerance in hexaploid wheat (Triticum aestivum L.) under control and drought stress condition. The germplasm was planted under control and stress conditions. The yield and yield components were recorded. QTLs were detected by linking morphological data with genotypic data. Five drought tolerant wheat lines were found based on a thousand-grain weight, ranging from 49 to 62 g. Novel Quantitative Trait Loci (QTLs) for spike length and grain per spike were identified during the present study. The novel QTLs are of great importance as these may be helpful in finding such regions in genome, which are responsible for drought tolerance contributing characteristics. The D genome is the main allele which denote drought tolerance to hexapoloid wheat while A and B genome in durum contribute the stress tolerant characteristics.

Recent Progress in Germplasm Evaluation and Gene Mapping to Enable Breeding of Drought-Tolerant Wheat

“…Different studies in wheat have shown the potential of DH lines as sources of stress tolerance. For example, Fatima et al (2018) identified five drought-tolerant DH lines out of 84, which were tested under control and drought stress conditions. However, DH lines are associated with negative effects from colchicine treatment, and also gametoclonal and somaclonal variation that affect plant performance.…”

“…Different studies in wheat have shown the potential of DH lines as sources of stress tolerance. For example, Fatima et al. (2018) identified five drought-tolerant DH lines out of 84, which were tested under control and drought stress conditions.…”

Molecular Characterization of Seedlings Derived from Two Polyemberyonic Egyptian Mango Cultivars Using SSR Markers

“…For the thirty two samples, other seven SSR markers produced total of 22 alleles with a high level of Polymorphism (~100 percent). The high ratio of polymorphism might be a consequence of replication slippage (Powell et al, 1996) or it might be due to low genetic stability and high variability of the DNA sequences in the amplified non-coding regions of the mango genome (Fatima et al, 2018).…”

Gene Expression and Molecular Differences in Two Wheat Genotypes under Salt and Drought Stresses

“…On other hand, Wmc179 marker was present as unique alleles only in Sakha93. This marker might be practical and advantageous to the genotype recognized to tolerance QTL markers alleles to utilize in marker assisted selection but it might be needed extra investigation by advanced techniques (Fatima et al, 2018). Moreover, in previous work of Chen et al (2012) revealed Wmc179 was amplified unique alleles relating to stripe rust in wheat.…”

“…This finding was in constant with O¨zlem et al (2018) and Piyusha & Naveen (2018). Expectably, SSR markers were detected 100% polymorphism as a consequence of replication slippage (Powell et al, 1996) or it might due to SSR markers that investigated for polymorphism among a set of genotypes (Fatima et al, 2018). Actually, each RAPD marker represent various genomic regions simultaneously and amplified huge number of fragments in one lane of gel and then the effective multiplex ratio was high (5.85) in RAPD markers.…”