Mapping quantitative trait loci associated with regeneration ability of seed callus in rice, Oryza sativa L.

Taguchi-Shiobara, Fumio; Lin, Shaoyang; Tanno, Ken-ichi; Komatsuda, Takao; Yano, Masahiro; Sasaki, Takuji; Oka, Seibi

doi:10.1007/s001220050632

Cited by 63 publications

(44 citation statements)

References 24 publications

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“…Abe and Futsuhara [16] reported that callus formation from mature seeds, and subsequent regeneration in 60 different japonica, indica and javanica cultivars indicated both intra and inter-varietal differences. Genetic markers have been used to find genes involved in the induction of embryogenic calli [35][36][37], but these genes have not yet been isolated [34]. In addition to genotype, various factors including physiological and developmental status of the explant, composition and concentration of the basal salts, organic components and plant growth regulators in the culture medium are known to influence callus induction and plant regeneration ability in rice [38].…”

Section: Resultsmentioning

confidence: 99%

High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake

Sah¹,

Kaur²

2014

J Rice Res

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A new and rapid protocol for production of embryogenic callus and plant regeneration has been reported in Oryza sativa cv. Kitaake. Callus cultures were established from seeds of Kitaake on MS medium supplemented with 2,4-D (3.0 mg/l), BAP (0.25 mg/l) and proline (0.6 g/l) gelled with phytagel (3.0 g/l), after 9 days of seed culturing. The embryogenic calli were regenerated on regeneration medium, i.e. MS medium supplemented with BAP (3.0 mg/l) and NAA (0.2 mg/l) and gelled with agar (8.0 g/l) in combination with phytagel (2.0 g/l) and regeneration occurred in 18 days. The regeneration percentage achieved was very high i.e 82.66% on the above medium. Rooting was achieved on half strength MS medium. The plantlets were hardened and transferred to soil in earthen pots. The regeneration protocol so developed through callus formation was highly reproducible. The plants showed normal growth and flowering under glass house conditions as well as in field conditions.

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Section: Resultsmentioning

confidence: 99%

High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake

Sah¹,

Kaur²

2014

J Rice Res

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“…The skewed distributions observed for all the characteristics analyzed in this study were also reported in many other papers dealing with mapping of the tissue culture response [13,34,35], and could be expected, as rye is generally a recalcitrant plant with respect to tissue culture response. The Bliss transformation, often applied in similar studies [10,35,36], improved the normality of distribution. Phenotypic measurements of the in vitro response of immature embryos expressed as the percentage of explants producing callus and the percentage of explants producing somatic embryos turned out to be a good basis for QTL analysis, as a total of 6 loci influencing the traits could be identified.…”

Section: Discussionmentioning

confidence: 99%

“…There are several examples of identifying QTLs for the in vitro response in cereals, e.g. in rice [10], wheat [11], maize [12] and barley [13]. No such study has thusfar been performed for rye.…”

Section: Introductionmentioning

confidence: 99%

The identification of QTLs associated with the in vitro response of rye (Secale cereale L.)

Bolibok

Gruszczynska

Hromada-Judycka

et al. 2007

Cellular and Molecular Biology Letters

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This study was conducted in order to identify quantitative trait loci (QTLs) for the in vitro culture response of winter rye (Secale cereale L.) immature embryos and immature inflorescences. A genetic linkage map comprising 67 SSRs, 9 ISSRs, 13 SAMPLs, 7 RAPDs, 2 SCARs and one EST marker was created based on the analyses of 102 recombinant inbred lines from the cross between lines L318 (which has a good response in tissue cultures) and L9 (which is unable to regenerate plants from somatic tissues and anthers). The map spans 979.2 cM, and the average distance between markers is 9.9 cM. Two characteristics were evaluated: callus induction (CI) and somatic embryogenesis ability (SE). They were expressed as the percentage of immature embryos/inflorescences producing callus (designated ECI/ICI) and the percentage of explants producing somatic embryos (ESE/ISE). All the analysed traits showed continuous variation in the mapping population but a non-normal frequency distribution. We identified nine putative QTLs controlling the tissue culture response of rye, explaining up to 41.6% of the total phenotypic variation: two QTLs for ECI — eci-1, eci-2; 4 for ESE — ece-1, ese-2, ese-3, ese-4; 2 for ICI — ici-1, ici2; and 1 for ISE — ise-1. They were detected on chromosomes 1R, 4R, 5R, 6R and 7R.

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“…Genetic variability has been utilized to identify novel factors that modulate the efficiency of regeneration in many plant species (Armstrong et al, 1992;Taguchi-Shiobara et al, 1997;Ben Amer et al, 1997;Flores Berrios et al, 2000;Mano and Komatsuda, 2002;Trujillo-Moya et al, 2011). A leucine-rich repeat receptor-like kinase, RECEPTOR-LIKE PROTEIN KINASE1 (RPK1), for instance, was identified as a major quantitative trait locus (QTL) that affects shoot regeneration in Arabidopsis accessions (Motte et al, 2014).…”

Section: Natural Variations That Impact Plant Regenerationmentioning

confidence: 99%

Plant regeneration: cellular origins and molecular mechanisms

et al. 2016

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Compared with animals, plants generally possess a high degree of developmental plasticity and display various types of tissue or organ regeneration. This regenerative capacity can be enhanced by exogenously supplied plant hormones in vitro, wherein the balance between auxin and cytokinin determines the developmental fate of regenerating organs. Accumulating evidence suggests that some forms of plant regeneration involve reprogramming of differentiated somatic cells, whereas others are induced through the activation of relatively undifferentiated cells in somatic tissues. We summarize the current understanding of how plants control various types of regeneration and discuss how developmental and environmental constraints influence these regulatory mechanisms.

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Mapping quantitative trait loci associated with regeneration ability of seed callus in rice, Oryza sativa L.

Cited by 63 publications

References 24 publications

High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake

High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake

The identification of QTLs associated with the in vitro response of rye (Secale cereale L.)

Plant regeneration: cellular origins and molecular mechanisms

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