Mapping quantitative trait loci conferring resistance to rice black-streaked virus in maize (Zea mays L.)

Abstract

Maize rough dwarf disease (MRDD) is one of the most serious virus diseases of maize worldwide, and it causes great reduction of maize production. In China, the pathogen was shown to be rice black-streaked virus (RBSDV). Currently, MRDD has spread broadly and leads to significant loss in China. However, there has been little research devoted to this disease. Our aims were to identify the markers and loci underlying resistance to this virus disease. In this study, segregation populations were constructed from two maize elite lines '90110', which is highly resistant to MRDD and 'Ye478', which is highly susceptible to MRDD. The F(2) and BC(1) populations were used for bulk sergeant analysis (BSA) to identify resistance-related markers. One hundred and twenty F(7:9) RILs were used for quantitative trait loci (QTL) mapping through the experiment of multiple environments over 3 years. Natural occurrence and artificial inoculation were both used and combined to determine the phenotype of plants. Five QTL, qMRD2, qMRD6, qMRD7, qMRD8 and qMRD10 were measured in the experiments. The qMRD8 on chromosome 8 was proved to be one major QTL conferring resistance to RBSDV disease in almost all traits and environments, which explained 12.0-28.9 % of the phenotypic variance for disease severity in this present study.

Identification of significant single nucleotide polymorphisms for resistance to maize rough dwarf disease in elite maize (Zea mays L.) inbred lines

“…Laodelphax striatellus Fallen Bai et al 2002;Zhang et al 2001;Fang et al 2001;Luan et al 2012). Yield losses of 30-100 % can be caused in severely infected fields yearly (Bai et al 2002;Shi et al 2012a).…”

“…In the past, various studies have focused on mapping QTLs for MRDD resistance based on linkage mapping, using bi-parental mapping populations such as F 2 , BC 1 and RIL populations, and many QTLs have been detected for MRDD resistance (Shi et al 2012a;Chen et al 2008;Luan et al 2012;Shi et al 2012b;Tao et al 2013). However, the detected QTLs were only partially consistent across the few mapping populations analyzed, which suggests that the genetic background of the studied population has a strong influence, and because of the few recombination events, large confidence intervals for QTLs and restricted alleles in the bi-parental genotypes, very few have been incorporated into maize resistance breeding programs (Van Inghelandt et al 2012;Riedelsheimer et al 2012b;Gupta et al 2005;Cardon and Bell 2001).…”

“…Maize rough dwarf disease (MRDD) is a devastating viral disease that is widely acknowledged to be an economically important biotic constraint in maize production regions of China and many other countries worldwide, severely affecting grain quality, causing great yield losses to total production of maize each year and posing a potential threat to global food security (Dovas et al 2004;Harpaz 1959;Luan et al 2012;Shi et al 2012a;Tao et al 2013). Phylogenetic analysis has indicated that MRDD is caused by the maize rough dwarf virus (MRDV) in Europe, Mal de Río Cuarto virus (MRCV) in South America and rice blackstreaked virus (RBSDV) in Asia, which belong to the Fijivirus genus in the family Reoviridae and are transmitted in a persistent manner by the plant-hopper Electronic supplementary material The online version of this article (doi:10.1007/s10681-014-1277-z) contains supplementary material, which is available to authorized users.…”

Control of Virus Diseases in Maize

“…2. At the distal end of the chromosome, QTLs for resistance to two maize-infecting fijiviruses and to the unrelated MCDV and MSpV were identified using different population structures and unrelated germplasm (Di Renzo et al, 2004;Luan et al, 2012;Martin et al, 2010;Zambrano, Jones, Brenner, et al, 2014). Overlapping QTLs for the nucleorhabdoviruses MMV and MFSV were identified in the inbred line Oh1VI in bin 2.08 (Zambrano, Jones, Brenner, et al, 2014).…”

“…Because MRDV and RBSDV are obligately transmitted by vectors, identification and development of highly resistant lines, hybrids, and cultivars have been slow. However, recent studies have identified strong quantitative trait loci (QTLs) for resistance in diverse germplasm (Luan, Wang, Li, Zhang, & Zhang, 2012;Shi et al, 2012;.…”