Mapping secondary substrate‐binding sites on the GH11 xylanase from Bacillus subtilis
Gustavo Avelar Molina,
Luis Felipe Santos Mendes,
Carlos Alessandro Fuzo
et al.
Abstract:Xylanases are of significant interest for biomass conversion technologies. Here, we investigated the allosteric regulation of xylan hydrolysis by the Bacillus subtilis GH11 endoxylanase. Molecular dynamics simulations (MDS) in the presence of xylobiose identified binding to the active site and two potential secondary binding sites (SBS) around surface residues Asn54 and Asn151. Arabinoxylan titration experiments with single cysteine mutants N54C and N151C labeled with the thiol‐reactive fluorophore acrylodan o… Show more
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