Mapping serum antibody repertoires using peptide libraries

Population-wide diversity and stability of serum antibody epitope repertoires against human microbiota

“…Several other antibody-profiling methods have been used to generate serological classifiers of disease and assess other biological parameters 46 . In our opinion, PhIP-Seq provides a good compromise among peptide length, library size, amenability for parallelized measurements and cost, while allowing for rational selection of the presented peptides (that is, not requiring the use of random peptides).…”

Systemic antibody responses against human microbiota flagellins are overrepresented in chronic fatigue syndrome patients

“…Conventional serological methods such as enzyme-linked immunosorbent assays (ELISAs) or peptide arrays allow analysis of hundreds or thousands of antigens in parallel (48), representing suitable strategies to test antibody responses against larger sets of candidate antigens of interest. However, in ME/CFS, a wide range of viral and bacterial antigens (including species of the gut microbiota) have been speculated to be involved (22)(23)(24)(25)(26)(27)(28)(29), with the gut microbiota especially representing a vast space of potential antigens [thousands of species, with each species encoding thousands of genes (49)].…”

Antibody signatures in inflammatory bowel disease: current developments and future applications

“…While most of the serological antibodies in IBD listed in Table 1 were identified using conventional enzyme-linked immunosorbent assays (ELISAs), several more advanced antibody profiling technologies have emerged [50] that have provided unprecedented insights into antibody repertoires in IBD (Figure 2). ELISAs, similar to radioimmunoassays (RIAs), are typically based on the immobilization of antigens on surfaces and subsequent detection of antibody binding by enzymatic (in ELISA) or radiolabeled (in RIA) signal amplification.…”