Mapping the spread of Avian Influenza Virus in Poultry at Polewali Mandar Regency, 2008-2013

Thaha, Aminah Hajah; Rauf, Juliawati; Bagenda, Isnaniah

doi:10.20956/jrvi.v2i1.4352

Cited by 2 publications

(1 citation statement)

References 9 publications

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“…AI viruses are resistant to environments with a certain period and temperature. Transmission occurs through direct contact and indirect contact (Adnyana, 2022;Martinez, Kencana, & Dibia, 2021;Rehman et al, 2023;Thaha, Rauf, & Bagenda, 2018).…”

Section: Introductionmentioning

confidence: 99%

Identification of Avian Influenza with the Real-Time Polymerase Chain Reaction (RT‒PCR) Method

Widyantari,

Kurniawan

2023

jharma

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Avian influenza is a zoonotic disease transmitted from poultry to humans. The prevalence of AI disease in Indonesia is likely to be a global health threat because of the ease of mutation in each generation, which makes pathogenicity higher. However, detection efforts still use conventional methods, so a more sophisticated detection search is needed. This research aims to identify the DNA amplification process and the results of detecting avian influenza virus using real-time PCR. Qualitative research using samples from BBVet Denpasar in May 2022. The samples were analyzed using the real-time polymerase chain reaction (RT‒PCR) method, and the results obtained were analyzed descriptively. The results show that the RT‒PCR method is very effective for detecting the presence of AI quantitatively and molecularly. A graph that crosses the threshold on cycles lower than 40 indicates that a positive sample will show nominal Ct. No nominal Ct was detected in this study, and the graph did not cross the threshold, so the model tested negative for the AI virus. The DNA amplification process of the avian influenza virus begins with an amplification process of 40 cycles, with each cycle stage in the form of denaturation at 94 °C for 30 seconds, annealing at 64 °C for 60 seconds, and DNA synthesis at 76 °C for 30 seconds. One cycle is declared if the amplification process has passed these three stages.

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Section: Introductionmentioning

confidence: 99%

Identification of Avian Influenza with the Real-Time Polymerase Chain Reaction (RT‒PCR) Method

Widyantari,

Kurniawan

2023

jharma

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Insidensi dan Risiko Penularan Avian Influenza pada Peternakan Ayam Petelur di Kabupaten Pinrang

Wahyuni

Wicaksono

Suprayogi

2022

J. Ilmu. Pertan. Indones.

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Avian Influenza (AI) is an infectious disease on poultry with a high mortality rate, caused by Avian Influenza type A viruses from the family Orthomyxoviridae. For the layer chicken, the negative impacts of AI are the decrease of egg production until the death. The aims of the study were to analyze disease’s case data, measure the spread of disease, describe the distribution of disease, and mapping the risk of AI occurrence. The data were collected from the records of Dinas Peternakan dan Perkebunan by conducting interviews using the structured questionnaires. The data were analysed by calculating the incidence rate and performing a geographic information system (GIS) for mapping. The results showed that the highest incidence rate among sub-districts for 3 years was in Mattiro Sompe sub-district of 2.015 cases per 10.000 animals-year, while the lowest incidence rate among 3 sub-districts for 3 years was in Duampanua, Lanrisang, and Mattiro Bulu sub-districts of 0 case per 10.000 animals-year. The highrisk district was Mattiro Sompe Subdistrict, so that the control measures need to be emphasized in this area by increasing the biosecurity practices, depopulation and stamping out in new infected area, vaccination, surveillance, routine socialization, monitoring, and provide additional animal health facilities and human resources, especially the veterinarians, to optimize the disease control measures. Keywords: Avian Influenza (AI), incidence rate, layer chicken, Pinrang district

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Mapping the spread of Avian Influenza Virus in Poultry at Polewali Mandar Regency, 2008-2013

Cited by 2 publications

References 9 publications

Identification of Avian Influenza with the Real-Time Polymerase Chain Reaction (RT‒PCR) Method

Identification of Avian Influenza with the Real-Time Polymerase Chain Reaction (RT‒PCR) Method

Insidensi dan Risiko Penularan Avian Influenza pada Peternakan Ayam Petelur di Kabupaten Pinrang

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