Marginal differences in preimplantation morphokinetics between conventional IVF and ICSI in patients with preimplantation genetic testing for aneuploidy (PGT-A): A sibling oocyte study

Munck, N De; Bayram, A; Elkhatib, Ibrahim; Abdala, Andrea; El-Damen, Ahmed; Arnanz, Ana; Melado, Laura; Lawrenz, Barbara; Fatemi, Human M.

doi:10.1371/journal.pone.0267241

Cited by 12 publications

(6 citation statements)

References 66 publications

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“…This anticipation time observed at pronuclei fading decreases as the embryo development continues and disappears from the 5-cell stage to the blastocyst stage. The statistically significant anticipations of pronuclei fading, as well as 2-cell and 3-cell stages in ICSI embryos compared to IVF embryos, were confirmed by other groups using sibling oocytes [ 29 ] and oocytes from different cohorts [ 30 , 31 ].…”

Section: Does Icsi Modify Embryo Development? Comparison With Convent...supporting

confidence: 59%

What Does Intracytoplasmic Sperm Injection Change in Embryonic Development? The Spermatozoon Contribution

Chamayou

Giacone

Cannarella

et al. 2023

JCM

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The intracytoplasmic sperm injection (ICSI) technique was invented to solve severe male infertility due to altered sperm parameters. Nowadays, it is applied worldwide for the treatment of couple infertility. ICSI is performed with any available spermatozoon from surgery or ejaculated samples, whatever are the sperm motility, morphology or quantity. The aim of the present review was to study if embryo development and kinetics would be modified by (1) ICSI under the technical aspects, (2) the micro-injected spermatozoa in connection with male infertility. From published data, it can be seen that ICSI anticipates the zygote kinetics Furthermore, because fertilization rate is higher in ICSI compared to conventional in vitro fertilization (IVF), more blastocysts are obtained for clinical use in ICSI. Sperm and spermatozoa characteristics, such as sperm parameters, morphology and vitality, DNA content (levels of sperm DNA fragmentation, microdeletions, and chromosomal abnormalities), RNA content, epigenetics, and sperm recovery site (testicular, epididymis, and ejaculated), have an impact on fertilization and blastocyst rates and embryo kinetics in different ways. Even though ICSI is the most common solution to solve couples’ infertility, the causes of male infertility are crucial in building a competent spermatozoa that will contribute to normal embryonic development and healthy offspring.

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Section: Does Icsi Modify Embryo Development? Comparison With Convent...supporting

confidence: 59%

What Does Intracytoplasmic Sperm Injection Change in Embryonic Development? The Spermatozoon Contribution

Chamayou

Giacone

Cannarella

et al. 2023

JCM

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“…Nowadays most reference laboratories require use of ICSI and blastocyst biopsy for PGT [25]. The results presented here suggest that 3PN rate do not affect the euploidy and aneuploid rate of the blastocyst.…”

Section: Discussionmentioning

confidence: 47%

Effect of three pro-nuclei (3PN) on the outcomes of PGT

Jiang

Yuan

et al. 2023

Preprint

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Aims To explore the influence of 3PN on outcomes in preimplantation genetic testing (PGT) cycles. Methods This was a retrospective study of patients undergoing PGT treatment included 139 PGT for aneuploidy (PGT-A) cycles and 285 cycles PGT for monogenic/single gene defects and/or chromosomal structural rearrangements (PGT-M/SR). The 424 PGT cycles were divided into two groups as follows: group 1 included 343 cycles with no 3PN zygotes and group 2 included 81 cycles with 3PN zygotes. 3PN cycle rate was compared between PGT-A and PGT-M/SR cycles. The outcomes of PGT were analyzed between the two groups. Results The female and male's age were significantly higher in PGT-A than PGT-M/SR cycles. Whereas 3PN rate was not significantly different between the two groups. The number of retrieved oocytes was significantly higher in 3PN group than Non 3PN group (13.98 ± 7.10 vs. 10.89 ± 6.65; P < 0.05). The MII oocyte rate, 2PN fertilizaion rate, D3 high embryo rate, biopsy blastocyst rate, per oocyte utilization and D5 blastocyst rate were significantly lower in 3PN group than Non 3PN group (P < 0.05). The results of chromosomal mosaic rate was significantly higher in 3PN group than Non 3PN group (16.3 vs. 11.9; P < 0.05). Conclusions No correlation was observed between 3PN formation rate and PGT-A or PGT-M/SR cycles. The occurrence of 3PN seems to impair the developing blastocyst and interfere with good embryo formation rate and mosaic rate in PGT. But the occurrence of 3PN does not seem to impair the euploid rate and aneuploid rate.

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“…This could be explained by the fact that even 30 s of gamete co-incubation for conventional IVF are enough to produce fertilization and clinical outcomes comparable to the longer traditional gamete co-incubation condition (Bungum et al, 2006). It is tempting to hypothesize that sperm penetration in IVF is faster than assumed and that the difference in time of insemination for IVF and ICSI is marginal (De Munck et al, 2022). What is more, pooling IVF and ICSI data and suggesting one time point for zygote freezing that accommodates both methods, would significantly facilitate simplification of routine laboratory practice.…”

Section: Discussionmentioning

confidence: 99%

The impact of zygote vitrification timing on pregnancy rate in frozen-thawed IVF/ICSI cycles

Makieva

Stähli

Xie

et al. 2023

Front. Cell Dev. Biol.

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Introduction: Cryopreservation of bipronuclear (2PN) stage zygotes is an integral part of IVF laboratory practice in countries with strict embryo culture legislation. Vitrification of zygotes is compatible with several strategies in infertility treatments holding a freeze-all indication and allows for effective workload management in settings with limited resources. Although it yields high survival rates and clinical outcomes, the ideal timing to commence vitrification of zygotes is elusive while it is empirically practiced in the window between 17 and 21 h post-insemination (hpi). We aimed to deduce the association between pregnancy rate and the time interval from insemination (IVF and ICSI) to vitrification to contribute to the standardization ofzygote cryopreservation.Methods: A retrospective analysis of data on vitrification timings and pregnancy outcomes collected between 2011 and 2019 was performed. All included women received an embryo transfer after warming of vitrified zygotes at the 2PN stage.Results: A total of 468 embryo transfers were included of which 182 (38.9%) resulted in pregnancy and 286 (61.1%) not. Vitrification was on average performed 18.74 ±0.63 hpi in the pregnant group and 18.62 ± 0.64 hpi in the non-pregnant group (OR 1.36, 95% CI 1.01; 1.83, p = 0.045). A multivariate analysis controlling for uterine pathologies, maternal age, AMH, the number of MII oocytes, previous history of pregnancy success, endometriosis, AFC, nicotine intake and male factor infertility showed no predictive value of vitrification timing on pregnancy rate. Three time intervals between insemination and vitrification were defined: 17:00 to 18:00 hpi (Group A), 18:01 to 19:00 hpi (Group B) and 19:01 to 21:00 hpi (Group C). Pregnancy occurred in 40/130 women (30.80%) in Group A, in 115/281 women (40.90%) in Group B and in 27/57 women (47.40%) in Group C. Univariate but not multivariate analysis showed a significantly higher pregnancy rate after the latest time interval between insemination and 2PN vitrification when compared to the earliest (Group C vs. A, OR 2.03, 95% CI 1.07; 3.84, p = 0.031).Discussion: These findings encourage further investigation on the impact of vitrification timing on clinical outcomes and hold the potential to standardize cryopreservation of zygotes from IVF/ICSI cycles to eventually improve the quality of long-term ART outcomes.

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Marginal differences in preimplantation morphokinetics between conventional IVF and ICSI in patients with preimplantation genetic testing for aneuploidy (PGT-A): A sibling oocyte study

Cited by 12 publications

References 66 publications

What Does Intracytoplasmic Sperm Injection Change in Embryonic Development? The Spermatozoon Contribution

What Does Intracytoplasmic Sperm Injection Change in Embryonic Development? The Spermatozoon Contribution

Effect of three pro-nuclei (3PN) on the outcomes of PGT

The impact of zygote vitrification timing on pregnancy rate in frozen-thawed IVF/ICSI cycles

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