Marinobacter confluentis sp. nov., a lipolytic bacterium isolated from a junction between the ocean and a freshwater lake

Abstract

A Gram-stain-negative, motile, aerobic and rod-shaped bacterium, designated HJM-18T, was isolated from the place where the ocean and a freshwater lake meet at Hwajinpo, South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain HJM-18T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 1.0–3.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain HJM-18T belonged to the genus Marinobacter. Strain HJM-18T exhibited 16S rRNA gene sequence similarity values of 97.05–98.22 % to the type strains of Marinobacter algicola, Marinobacter flavimaris, Marinobacter adhaerens, Marinobacter salarius, Marinobacter salsuginis, Marinobacter guineae and Marinobacter gudaonensis and of 93.21–96.98 % to the type strains of the other species of the genus Marinobacter. Strain HJM-18T contained Q-9 as the predominant ubiquinone and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C18 : 1ω9c as the major fatty acids. The major polar lipids detected in strain HJM-18T were phosphatidylethanolamine, phosphatidylglycerol and one unidentified aminophospholipid. The DNA G+C content was 58 mol% and the mean DNA–DNA relatedness values with the type strains of the seven phylogenetically related species of the genus Marinobacter were 10–27 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain HJM-18T is separated from recognized species of the genus Marinobacter. On the basis of the data presented, strain HJM-18T represents a novel species of the genus Marinobacter, for which the name Marinobacter confluentis sp. nov. is proposed. The type strain is HJM-18T ( = KCTC 42705T = NBRC 111223T).

Marinobacter lipolyticusfrom Red Sea for lipase production and modulation of silver nanomaterials for anti‐candidal activities

“…2). Previous studies demonstrated a positive lipolytic activity of Marinobacter confluentis , which was isolated from the junction between fresh water and marine water [50, 52]. In our study, the bacterial strain grown in a liquid culture medium and a significant lipolytic activity −2.105 nkatml −1 was detected in the supernatant.…”

“…In previous reports, a bacterial strain of Aneurinibacillu s sp. has shown a significant variable activity in different screening methods on different media supplemented with olive oil as a carbon source [52].…”

Marinobacter denitrificans sp. nov., isolated from marine sediment of southern Scott Coast, Antarctica

“…confluentis KCTC 42705 T or M. halotolerans CP12 T [41, 42]. The sole respiratory quinone of strain JB02H27 T was ubiquinone-9 (Q-9, 100 % determined by HPLC), consistent with the other members of the genus Marinobacter [41–43].…”

Divergent Genomic Adaptations in the Microbiomes of Arctic Subzero Sea-Ice and Cryopeg Brines

“…We observed a significantly higher abundance of phosphatidylglycerol lysyltransferase (mprF) genes in cryopeg communities, used by bacteria to alter the net charge of their cellular envelope and resist a variety of cationic antimicrobials (Ernst et al, 2009), as well as for osmoprotection (Dare et al, 2014). As phosphatidylglycerol is a major building block of Marinobacter membranes (Park et al, 2015;Zhong et al, 2015), FIGURE 6 | Schematic of genomic adaptations to life in subzero hypersaline sea-ice brines (upper panels) and cryopeg brines (lower panels). Sea ice, exposed to atmospheric conditions and in exchange with underlying seawater (left), is characterized by vertical gradients in temperature, salinity and nutrient availability and subject to temporal disturbances of these conditions.…”