Marinobacter panjinensis sp. nov., a moderately halophilic bacterium isolated from sea tidal flat environment

Abstract

Two moderately halotolerant bacterium strains, designated PJ-16T and PJ-38, were isolated from a tidal flat of the red beach in Panjin City, Liaoning Province, PR China. Cells were found to be Gram-stain-negative, aerobic, motile, rod-shaped with a single polar flagellum. Optimum growth of strain PJ-16T occurred at 30 °C, pH 7.0 and 0.2–8.0 % (w/v) NaCl, and strain PJ-38 at 30 °C, pH 6.0–7.0 and 0.2–8.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain PJ-16T was most closely related to Marinobacter denitrificans KCTC 62941T (99.2 % 16S rRNA gene sequence similarity), Marinobacter algicola DSM 16394T (98.6 %), Marinobacter salarius JCM 19399T (98.4 %) and Marinobacter confluentis KCTC 42705T (98.2 %), and strain PJ-38 was most closely related to M. denitrificans KCTC 62941T (99.1 %), M. algicola DSM 16394T (98.6 %), M. salarius JCM 19399T (98.4 %) and M. confluentis KCTC 42705T (98.1 %). The G+C content of the genomic DNA of strain PJ-16T based on its draft genomic sequence was 57.4 mol%. The major cellular fatty acids of strain PJ-16T were C16 : 0, C16 : 1 ω7c/C16 : 1 ω6c and C18 : 1 ω9c. The major respiratory quinone of PJ-16T was ubiquinone-9 and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The results of the phenotypic, phylogenetic and genomic analyses revealed that strains PJ-16T and PJ-38 represent a novel species of the genus Marinobacter , and the name Marinobacter panjinensis sp. nov. is proposed. The type strain is PJ-16T (= CGMCC 1.13694T= KCTC 72023T).

Marinobacter azerbaijanicus sp. nov., a moderately halophilic bacterium from Urmia Lake, Iran

“…Marinobacter members have been isolated from various environments, such as marine sediments [4,5], saline soils [6], mangrove sediments [7], marine salterns [8], surface seawater [9], or offshore sediments [10]. Members of this genus require salt for their growth; therefore, they are slight or moderate halophiles [11][12][13][14][15]. Many studies have pointed out the biodiversity and ecological significance of members of the genus Marinobacter.…”

Draft Genome Sequence Analyses of Two Novel Marinobacter suadae sp. nov. and Wenyingzhuangia gilva sp. nov. Isolated from the Root of Suaeda japonica Makino

“…The genus Marinobacter consists of 60 species at present, and several child taxa have been validly published ( accessed on 1 November 2023) [ 8 ]. Marinobacter is a metabolically flexible genus that inhabits a very wide range of marine, tidal, and sediment environments [ 7 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ]. These bacteria are mostly heterotrophic; however, in some cases, they are mixotrophic [ 9 , 18 , 19 ].…”