Mark Twain: How to fathom the depth of your pet proteome

Targeted mass spectrometry analysis of the proteins IGF1, IGF2, IBP2, IBP3 and A2GL by blood protein precipitation

“…This problem is further aggravated when employing fast LC methods for high-throughput analysis that have reduced capacity for analyte separation and therefore higher signal suppression and matrix effects. Thus, the development of simplified and optimised blood sample preparation protocols prior to LC-MS/ MS analysis remains a highly active area of research [9][10][11][12][13][14][15][16].…”

Analytical Approaches for the Characterization and Identification of Olive (Olea europaea) Oil Proteins

“…Nevertheless, the eluates of the CPLL captures resulted in clean lanes with no band appearing in the gel. This could be explained taking into account that the interaction between proteins and CPLLs are prominently ionic , and they may be extremely hampered in such an apolar matrix.…”

Combinatorial Peptide Ligand Library and two dimensional electrophoresis: New frontiers in the study of peritoneal dialysis effluent in pediatric patients

“…CPLL has been recently described as an efficient approach to decrease the concentration of dynamic range of complex protein mixtures and at the same time increase the capture of previously undetected proteins [5,6]. Each CPLL bead contains a unique hexapeptide ligand which can theoretically interact with one or a few proteins.…”