MARK1 is a Novel Target for miR-125a-5p: Implications for Cell Migration in Cervical Tumor Cells

Martínez-Acuña, Natalia; González-Torres, Alejandro; Virginia, Tapia-Vieyra Juana; Álvarez-Salas, Luis Marat

doi:10.2174/2211536606666171024160244

Cited by 32 publications

(20 citation statements)

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“…Current research on miR-222-5p has focused on the role of angiogenesis in the endothelium [21,22], and few studies investigated the effect of miR-222-5p in cancers. As down-regulated miRNAs in GAC, miR-125-5p was identified as a tumor suppressor in glioblastoma [23], cervical cancer [24], and renal cell carcinoma [25], which was involved in proliferation, migration, and apoptosis. More researches have investigated the role of miR-145-3p in cancers, such as bladder cancer [26], lung squamous cell carcinoma [27], gallbladder cancer [28], and head and neck squamous cell carcinoma [29], which is also considered as a tumor suppressor.…”

Section: Discussionmentioning

confidence: 99%

miR-96-5p enhances cell proliferation and invasion via targeted regulation of ZDHHC5 in gastric cancer

Wang¹,

Liu

Meng

2020

Bioscience Reports

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Objective: To explore the biological function and mechanism of miR-96-5p in gastric cancer. Methods: The expression of differently expressed microRNAs (DEMs) related to gastric adenocarcinoma (GAC) prognosis was identified in GAC tumor samples and adjacent normal samples by qRT-PCR. A target gene miR-96-5p was selected using TargetScan, miRTarBase, miRDB databases. The combination of miR-96-5p and ZDHHC5 was verified by luciferase receptor assay. To further study the function and mechanism of miR-96-5p, we treated MGC-803 cells with miR-96-5p inhibitor and si-ZDHHC5, then detected cell viability, apoptosis, migration and invasion ability, as well as the expression of ZDHHC5, Bcl-2, Bax, cleaved caspase-3, cleaved caspase-9, and COX-2 by Western blot. Results: Compared with adjacent normal samples, the levels of miR-96-5p, miR-222-5p, and miR-652-5p were remarkably increased, while miR-125-5p, miR-145-3p, and miR-379-3p were significantly reduced in GAC tumor samples (P<0.01), which were consistent with bioinformatics analysis. Furthermore, ZDHHC5 was defined as a direct target gene of miR-96-5p. miR-96-5p silence significantly reduced cell viability, increased cell apoptosis, and suppressed cell migration and invasion, as well as inhibited the expression of Bcl-2 and COX-2 and promoted Bax, cleaved caspase-3 and cleaved caspase-9 level in MGC-803 cells (P<0.01). Notably, ZDHHC5 silence reversed the inhibiting effects of miR-96-5p on MGC-803 cells growth and metastasis Conclusion: Our findings identified six microRNAs (miRNAs; miR-96-5p, miR-222-5p, miR-652-5p, miR-125-5p, miR-145-3p, and miR-379-3p) related to GAC prognosis, and suggested that down-regulated miR-96-5p might inhibit tumor cell growth and metastasis via increasing ZDHHC5 expression enhance MGC-803 cell apoptosis, as well as decrease MGC-803 cell metastasis.

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Section: Discussionmentioning

confidence: 99%

miR-96-5p enhances cell proliferation and invasion via targeted regulation of ZDHHC5 in gastric cancer

Wang¹,

Liu

Meng

2020

Bioscience Reports

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“…Sample viability was assessed by PCR using β-actin primers [8]. HPV genomic DNA was evaluated using the MY09/MY11 primers as previously described in [9].…”

Section: Dna Viabilitymentioning

confidence: 99%

A Pilot Study on the HPV Type Frequency in a Federal High-Specialty Hospital of Mexico City: Is HPV16 Our Main Problem?

Rangel-Guerrero¹,

Basilio

Rocío

et al. 2020

SN Compr. Clin. Med.

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Human papillomavirus (HPV) is the most common sexually transmitted infection worldwide and a necessary condition for cervical cancer (CeCa) development. HPV types 16 and 18 are the most common in cervical lesions (CLs); therefore, specific vaccines have reduced cervical neoplasia. In 2015, HPV16 presented the highest frequency in CLs (~28%); however, types 31, 45, 52, and 58 were also detected, suggesting that other HPV types may have impact on healthcare. Here, we typified 30 patients with CLs from the "Hospital Regional 1°de Octubre" (HR1O), in Mexico City, for the most reported high-risk papillomavirus (HPV16, 18, 31, 33, 45, 58, and 59). The results showed that HPV16 frequency is dissimilar to international and previous national data, suggesting that a deeper knowledge of HPV type distribution is required before choosing a vaccination scheme for HPV in Mexico City.

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“…More importantly, the abilities of cell proliferation, migration and invasion are the key factors leading to poor prognosis and low survival rate of thyroid squamous cell carcinoma. MicroRNAs (miRNAs), a class of noncoding and small RNAs, play a critical role in gene expression by targeting binding of the 3'-untranslated region, and thus could regulate cellular biological processes such as cell growth, cycle, and apoptosis [6,7]. Recently, more and more miRNAs have been found to be involved in the development of tumours and effected the metastasis of tumours [8].…”

Section: Introductionmentioning

confidence: 99%

miR-597-3p inhibits invasion and migration of thyroid carcinoma SW579 cells by targeting RAB23

Wen

Lin

et al. 2021

Endokrynologia Polska

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MARK1 is a Novel Target for miR-125a-5p: Implications for Cell Migration in Cervical Tumor Cells

Abstract: The results showed MARK1 as a novel functional target for miR-125a-5p with implications on cell migration of tumor cervical cancer cells.

Cited by 32 publications

References 0 publications

miR-96-5p enhances cell proliferation and invasion via targeted regulation of ZDHHC5 in gastric cancer

miR-96-5p enhances cell proliferation and invasion via targeted regulation of ZDHHC5 in gastric cancer

A Pilot Study on the HPV Type Frequency in a Federal High-Specialty Hospital of Mexico City: Is HPV16 Our Main Problem?

miR-597-3p inhibits invasion and migration of thyroid carcinoma SW579 cells by targeting RAB23

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