Marker-Aided Selection of Polyploid Poplars

Kong, Fanming; Liu, Jingjing; Chen, Ying-Nan; Wan, Zhibing; Yin, Tongming

doi:10.1007/s12155-013-9331-6

Cited by 6 publications

(4 citation statements)

References 17 publications

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“…We did not observe any variation in SSR patterns among ramets of the same elite clonal lineage. Nuclear microsatellites along with other classes of molecular markers are employed also for determination of ploidy level in poplars [ 33 , 34 ], and indications of triploidy were found by us with respect to several genotypes. Triploid aspen hybrids often demonstrate increased vigor and resistance to pathogens, so this matter should be further studied by means of karyological and floating cytometry analysis.…”

Section: Resultsmentioning

confidence: 99%

Application of Microsatellite Loci for Molecular Identification of Elite Genotypes, Analysis of Clonality, and Genetic Diversity in Aspen Populus tremula L. (Salicaceae)

Политов

Belokon

et al. 2015

International Journal of Plant Genomics

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Testing systems for molecular identification of micropropagated elite aspen (Populus tremula L.) genotypes were developed on the base on microsatellite (SSR) loci. Out of 33 tested microsatellite loci, 14 were selected due to sustainable PCR amplification and substantial variability in elite clones of aspen aimed for establishment of fast-rotated forest plantations. All eight tested clones had different multilocus genotypes. Among 114 trees from three reference native stands located near the established plantations, 80 haplotypes were identified while some repeated genotypes were attributed to natural clones which appeared as a result of sprouting. The selected set of SSR markers showed reliable individual identification with low probability of appearance of identical aspen genotypes (a minimum of 4.8 · 10−10 and 1 × 10−4 for unrelated and related individuals, resp.). Case studies demonstrating practical applications of the test system are described including analysis of clonal structure and levels of genetic diversity in three natural aspen stands growing in the regions where plantations made of elite clones were established.

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Section: Resultsmentioning

confidence: 99%

Application of Microsatellite Loci for Molecular Identification of Elite Genotypes, Analysis of Clonality, and Genetic Diversity in Aspen Populus tremula L. (Salicaceae)

Политов

Belokon

et al. 2015

International Journal of Plant Genomics

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“…At the same time, it is beneficial to improve the effectiveness of polyploidy breeding in the genus Populus, according to the experience of parent selection in hybridization breeding [76]. Polyploidization is essential in speciation, evolution, and creating elite varieties for poplar plantations [77][78][79]. Moreover, triploid Populus is more widely used for the production of lumber, fiber, and fuel, in contrast to diploids [80,81].…”

Section: Discussionmentioning

confidence: 99%

Variation of Traits on Seeds and Germination Derived from the Hybridization between the Sections Tacamahaca and Aigeiros of the Genus Populus

Zhu

Tian²,

Wang

et al. 2018

Forests

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Poplar is an important research organism, and species in sections Tacamahaca and Aigeiros, have advantages in terms of stress resistance, ease of propagation, and fast growth. Poplar species are widely distributed and well-adapted in the world, presenting a large potential for genetic improvement. Hybridization between different species allows us to generate offspring with a unique combinations of traits. This approach has a huge potential for breeding new poplar varieties that could aid in controlling desertification in the arid and semi-arid zones of the “Three-North” in China. In this study, we carried out a cross test scheme with nine female and thirteen male poplar trees. A total of 105,401 seeds were collected from 117 crosses. Flowering phenology and seed maturation differences of the hybrid progeny were monitored in greenhouses. For male trees, Populus deltoides had the longest flowering time. For female trees, Populus pseudo-simonii showed the longest seed maturity time. The number of carpals and ovules were not the same in different females. Meanwhile, three carpals were found in P. pseudo-simonii. A highly significant positive correlation was found between the seed size and the Thousand Kernel Weight, as well as the seedling cotyledon length. During seed germination, non-radicle and non-hypocotyl seedlings were observed. We also observed a number of cotyledon variants, including single and fused cotyledons, two cotyledons with one cotyledon cracking into two parts, three cotyledons, as well as four cotyledons. These results lay a favorable foundation for combining the research between the sections Tacamahaca and Aigeiros in future work.

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“…However, marker-aided selection enables us to narrow down the polyploids to a small number of candidates, and thus greatly improves the efficiency of FCM analysis. Kong et al 31. demonstrated the power of the combining marker-aided selection and FCM for screening polyploid poplars.…”

Section: Discussionmentioning

confidence: 99%

“…Using fully informative molecular markers, we can identify polyploid candidates based on the observed allele numbers, and the candidates can then be confirmed by FCM analysis. This combined approach was shown to be very efficient for discriminating polyploids in natural poplar stands31. Additionally, an effective method for screening polyploids is also highly desirable for willow breeding programs.…”

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confidence: 99%

An analytical toolkit for polyploid willow discrimination

Guo

Hou

Yin

et al. 2016

Sci Rep

Self Cite

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Polyploid breeding is an important means for creating elite willow cultivars, and therefore provokes an active demand for discriminating the ploidy levels of natural willow stands. In this study, we established an analytical toolkit for polyploid willow identification by combining molecular markers and flow cytometry (FCM). A total of 10 single-copy fully informative SSRs were chosen for marker-aided selection based on a segregation test with a full-sib willow pedigree and a mutability test with a collection of natural willow stands. Aided by these molecular markers, we performed polyploid selection in two tree species and two shrub species of the genus Salix. The ploidy levels of the investigated samples were further examined using a flow cytometer. It was previously shown that results from marker-aided selection were consistent with those from FCM measurements. Based on ploidy level assessment in different willow species, it was found that tree willows were dominantly tetraploid, whereas shrub willows were most frequently diploid. With this analytical toolkit, polyploids can be rapidly screened from a large number of natural stands; thereafter, the exact ploidy levels of the polyploid candidates can be efficiently confirmed by FCM. This analytical toolkit will greatly enhance polyploid breeding programs for willows.

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Marker-Aided Selection of Polyploid Poplars

Cited by 6 publications

References 17 publications

Application of Microsatellite Loci for Molecular Identification of Elite Genotypes, Analysis of Clonality, and Genetic Diversity in Aspen Populus tremula L. (Salicaceae)

Application of Microsatellite Loci for Molecular Identification of Elite Genotypes, Analysis of Clonality, and Genetic Diversity in Aspen Populus tremula L. (Salicaceae)

Variation of Traits on Seeds and Germination Derived from the Hybridization between the Sections Tacamahaca and Aigeiros of the Genus Populus

An analytical toolkit for polyploid willow discrimination

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