Marker-assisted breeding for Downy mildew, Powderey mildew and Phylloxera resistance at FEM

Vezzulli, S.; Dolzani, C.; Nicolini, Daniela; Bettinelli, Paola; Migliaro, Daniele; Gratl, Verena; Stedile, T.; Zatelli, A.; Dallaserra, M.; Clementi, S.; Dorigatti, C.; Velasco, Riccardo; Zulini, L.; Stefanini, M.

doi:10.1051/bioconf/20191301002

Cited by 3 publications

(3 citation statements)

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“…The use of F 1 families derived from the cross of two parents with contrasting phenotypes is the most used strategy for genetic mapping in grapevines [49]. Based on the pseudo-testcross strategy [50], it is suitable for highly heterozygous plants with long juvenile periods, such as grapevines.…”

Section: Mapping Resistance Genes For Powdery Mildew Resistance Using...mentioning

confidence: 99%

Powdery Mildew Resistance Genes in Vines: An Opportunity to Achieve a More Sustainable Viticulture

et al. 2022

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Grapevine (Vitis vinifera) is one of the main fruit crops worldwide. In 2020, the total surface area planted with vines was estimated at 7.3 million hectares. Diverse pathogens affect grapevine yield, fruit, and wine quality of which powdery mildew is the most important disease prior to harvest. Its causal agent is the biotrophic fungus Erysiphe necator, which generates a decrease in cluster weight, delays fruit ripening, and reduces photosynthetic and transpiration rates. In addition, powdery mildew induces metabolic reprogramming in its host, affecting primary metabolism. Most commercial grapevine cultivars are highly susceptible to powdery mildew; consequently, large quantities of fungicide are applied during the productive season. However, pesticides are associated with health problems, negative environmental impacts, and high costs for farmers. In paralleled, consumers are demanding more sustainable practices during food production. Therefore, new grapevine cultivars with genetic resistance to powdery mildew are needed for sustainable viticulture, while maintaining yield, fruit, and wine quality. Two main gene families confer resistance to powdery mildew in the Vitaceae, Run (Resistance to Uncinula necator) and Ren (Resistance to Erysiphe necator). This article reviews the powdery mildew resistance genes and loci and their use in grapevine breeding programs.

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Section: Mapping Resistance Genes For Powdery Mildew Resistance Using...mentioning

confidence: 99%

Powdery Mildew Resistance Genes in Vines: An Opportunity to Achieve a More Sustainable Viticulture

et al. 2022

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“…Asma ıslahı günümüzde külleme dahil olmak üzere farklı direnç programları ile devam etmektedir (Burger ve ark., 2009;Eibach ve Töpfer, 2015;Di Gaspero ve Foria, 2015). Bu çalışmalarda dirençli çeşit ve genotiplerin erken dönemde belirlenmesi için çeşitli markörler kullanılmaktadır (Dalbó ve ark., 2001;Pauquet ve ark., 2001;Riaz ve ark., 2011;Vezzulli ve ark., 2019). Bu markörler yardımı ile külleme gibi hastalıklara dirençli tür ve çeşitlerin genetik testlemeleri yapılmış ve bu testlemelerde dirençle ilişkili genler ve allel büyüklükleri belirlenmiştir (Fischer ve ark., 2004;Monteiro ve ark., 2013;Zyprian ve ark.,2016;Shidfar ve ark., 2019;Yıldırım ve ark., 2019;Akkurt ve ark., 2022).…”

Section: Introductionunclassified

Bazı Melez Asma Genotiplerinin Küllemeye Duyarlılıklarının Küllemeye Dayanıklılıkla İlişkili Bazı Markörler ile İncelenmesi

Bozkurt,

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2024

Türk Tarım Ve Doğa Bilimleri Dergisi

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Bu çalışma 2022-2023 yılları arasında Tokat Gaziosmapaşa Üniversitesi, Tarımsal Uygulama ve Araştırma Merkezine ait seralarda yürütülmüştür. Çalışmada 6 adet Narince × Kishmish Vatkana (NKV) ve 66 adet Narince × Regent (NRG) melezi Ren1, Ren3 ve Ren9 lokusları yönünden MAS yöntemiyle küllemeye dayanıklılıkla ilişkili markörler ile taranmıştır. PCR amplifikasyon ürünleri agaroz jel üzerinde görüntülenerek, örnekler bant var (+) bant yok (-) olarak incelenmiştir. Çalışma sonucunda; genlerin bant görüntü sonuçları genotipler arasında değişkenlik göstermiştir. NRG-7, NRG-146, NRG-183, NRG-195, NRG-196, NRG-197 ve NRG-200 genotiplerinin bant görüntü sonuçları, bu genotiplerde daha önce yapılmış olan külleme skorlamaları sonuçları ile uyumlu tespit edilmiştir. NRG-146 ve NRG-195 genotipleri; Ren1, Ren3, Ren9, NRG-7; Ren3 ve Ren9, NRG-183 ve NRG-196; Ren9, NRG-197; Ren1, Ren9, ve NRG-200 genotipi Ren1 ve Ren3 lokusları bakımından bant (+) vermiştir. Son yıllarda, küllemeye karşı direnç genleri veya lokuslarının araştırılmasına olan ilgi artmış ve yeni direnç genleri veya lokusları keşfedilmiştir. Ren lokuslarında, külleme direncinden ve bunların etki mekanizmalarından hangi genlerin sorumlu olduğuna yönelik çalışmalar doğal olarak direnç genleri taşıyan üzüm çeşitlerinin geliştirilmesi, pestisit kullanımının azaltılması bakımından hayati derecede önem taşımaktadır.

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“…Downy mildew, caused by Plasmopara viticola, and powdery mildew, caused by Erysiphe necator, are the most common and economically significant fungal diseases of the vine. The determination of resistance genes in grapevines to these pathogens is carried out mainly in all world scientific centers [1][2][3][4][5][6][7][8][9]. Identification of genes for resistance to downy mildew and powdery mildew in domestic material with the aim of creating stable ampelocenoses, preserving the harvest and extending the productive life of grape plantations is an urgent task.…”

Section: Introductionmentioning

confidence: 99%

Potential of genetic resistance of new table grape hybrids to fungal pathogens

2021

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Downy mildew (Plasmopara viticola) and powdery mildew (Erysiphe necator) are the most common and economically significant fungal diseases in vineyards. The task of this work is to study the genotypes of new promising hybrid forms of table grapes for the presence of resistance genes to downy mildew (Rpv10 and Rpv3) and powdery mildew (Ren9) using DNA-markers. The study was carried out on table grape hybrids under the working names Agat dubovskiy, Akelo, Arabella, Artek, Dubovskiy rozovyi, Gamlet, Ispolin, Kishmish dubovskiy, Kurazh, Pestryi, Valensiya and registered variety Liviya. The studied genes were analyzed using markers UDV305 and UDV737 (Rpv3), GF09-46 (Rpv10), CenGen6 (Ren9). The following cultivars were used as reference genotypes: Saperavi severnyi (carries Rpv10 gene) and Regent (Rpv3 and Ren9). It was established that Rpv3 gene is carried by hybrids Kishmish dubovskiy, Agat dubovskiy, Kurazh, Valensiya, Akelo, Gamlet, Dubovskiy rozovyi, Pestryi. Ren9 gene was found in Artek, Agat dubovskiy, Kurazh, Ispolin, Valensiya, Arabella, Gamlet, Dubovskiy rozovyi, Pestryi. The Rpv10 gene was not detected in any of the analyzed grapevine samples. genotypes Agat dubovskiy, Kurazh, Gamlet, Dubovskiy rozovyi, Pestryi, Valensiya carry Rpv3 and Ren9 genes simultaneously. These grapevines have an elegant bunch and large berries that are attractive to consumers.

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Marker-assisted breeding for Downy mildew, Powderey mildew and Phylloxera resistance at FEM

Cited by 3 publications

References 5 publications

Powdery Mildew Resistance Genes in Vines: An Opportunity to Achieve a More Sustainable Viticulture

Powdery Mildew Resistance Genes in Vines: An Opportunity to Achieve a More Sustainable Viticulture

Bazı Melez Asma Genotiplerinin Küllemeye Duyarlılıklarının Küllemeye Dayanıklılıkla İlişkili Bazı Markörler ile İncelenmesi

Potential of genetic resistance of new table grape hybrids to fungal pathogens

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