Marker assisted introgression of multiple genes for bacterial blight resistance into aromatic Myanmar rice MK-75

Win, KyawKyaw; Korinsak, Siripar; Sirithunya, P.; Lanceras-Siangliw, J.; Jamboonsri, Watchareewan; Da, Tao; Patarapuwadol, Sujin; Toojinda, Theerayut

doi:10.1016/j.fcr.2013.08.002

Cited by 22 publications

(8 citation statements)

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“…On the other hand, rice varieties carrying multiple resistance genes provide a more effective and durable resistance towards diverse Xoo strains and hence are best suited for long term and large-scale cultivation. For example, [79] reported about xa5 , xa13 and Xa21 gene combination providing superior resistance in Eastern India, [80] informed about Xa4 , Xa7 and Xa21 gene combination showing higher resistance in Vietnam, [81] identified xa5 , Xa21 ; xa33 , xa5 , xa33 ; xa5 , Xa21 ; Xa21 , xa33 gene combinations conferring wider and higher resistance in Myanmar and Thailand, [5] found that Xa4 , xa5 and Xa21 pyramided line exert a durable and long term resistance than a single R gene in Korea while, [36] reported about Xa4 and Xa7 gene combination was durable against Xoo at high temperature and [82] conveyed that Xa4 , xa5 and Xa21 gene combination was superior than any of the single R gene. Likewise, in the present study we also observed that germplasm with multiple genes typically confer higher resistance than germplasm with single gene.…”

Section: Discussionmentioning

confidence: 99%

“…For example,[79] reported about xa5, xa13 and Xa21 gene combination providing superior resistance in Eastern India,[80] informed about Xa4, Xa7 and Xa21 gene combination showing higher resistance in Vietnam,[81] identified xa5, Xa21; xa33, xa5, xa33; xa5, Xa21; Xa21, xa33gene combinations conferring wider and higher resistance in Myanmar and Thailand,[5] found that Xa4, xa5 and Xa21 pyramided line exert a durable and long term resistance than a single R gene in Korea while,[36] reported about Xa4 and Xa7 gene combination was durable against…”

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confidence: 99%

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Exploring of bacterial blight resistance in landraces, mining of resistant gene(s) and genetic divergence of resistant germplasm through molecular markers and quantitative traits

Anik¹,

Nihad²,

Hasan³

et al. 2021

Preprint

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Bacterial blight, one of the oldest and severe diseases of rice poses a major threat towards global rice production and food security. Thereafter, sustainable management of this disease has given paramount importance globally. In the current study, we explored 792 landraces to evaluate their disease reaction status against three highly virulent strains ( BXo69 , BXo87 and BXo93 ) of Xanthomonas oryzae pv . oryzae ( Xoo ). Subsequently, we intended to identify the possible candidate resistant ( R ) genes responsible for the resistant reaction using six STS markers correspond to Xa4 , xa5 , Xa7 , xa13 , Xa21 and Xa23 genes and finally, we evaluated morphological and molecular diversity of the potential bacterial blight resistant germplasm using quantitative traits and ISSR markers. Based on pathogenicity test, a single germplasm was found as highly resistant while, 33 germplasm were resistant and 40 were moderately resistant. Further molecular study on these 74 germplasm divulged that 41 germplasm carried Xa4 gene, 15 carried xa5 gene, 62 carried Xa7 gene, 33 carried xa13 gene, and 19 carried Xa23 gene. Only a single germplasm consisted of Xa21 gene. Interestingly, we found a wide range of gene combinations ranged from 2 to 4 genes among the germplasm resistant to bacterial blight and G3 genotype (Acc. No. 4216; highly resistant) having Xa4 , Xa7 , xa13 , Xa21 and G43 genotype (Acc. No. 1523; resistant) having Xa4 , xa5 , xa13 and Xa23 gene combination being the most effective against all the Xoo strains. Nonetheless, UPGMA dendrogram and heatmap analysis based on quantitative traits identified two important clusters viz. cluster-III and cluster-VIII with multiple desired traits However, genetic similarity based on ISSR marker data pointed out 3 germplasm, namely, G20 (Acc. No. 4004; contained Xa4, Xa7, xa13 genes), G17 (Acc. No. 3981; contained Xa7, xa13 genes) and G6 (Acc. No. 991; contained Xa4, Xa7, xa13, Xa23 genes) having comparatively lower genetic similarity (0.30, 0.37 and 0.38, respectively) with elite variety BRRI dhan28. Notably, Mantle test of molecular and morphological data indicated that there was a positive correlation (r = 0.113) between them. The outcome of this study would enrich and diversify the rice gene pool and would be useful for the development of durable bacterial blight resistant varieties.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Exploring of bacterial blight resistance in landraces, mining of resistant gene(s) and genetic divergence of resistant germplasm through molecular markers and quantitative traits

Anik¹,

Nihad²,

Hasan³

et al. 2021

Preprint

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“…Numerous resistance (R) genes for BLB have been discovered during the last 50 years. At least 43 resistance genes (labelled with R prefixes) have been identified and characterized from various rice accessions including its wild relatives [ 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ]. Of these, 16 genes reportedly function as recessive genes with the rest characterized as dominant [ 37 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Bacterial Blight Resistance Loci in Rice (Oryza sativa L.) against Diverse Xoo Thai Strains by Genome-Wide Association Study

Korinsak

Darwell

Wanchana

et al. 2021

Plants

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Bacterial leaf blight (BLB) is a serious disease affecting global rice agriculture caused by Xanthomonas oryzae pv. oryzae (Xoo). Most resistant rice lines are dependent on single genes that are vulnerable to resistance breakdown caused by pathogen mutation. Here we describe a genome-wide association study of 222 predominantly Thai rice accessions assayed by phenotypic screening against 20 Xoo isolates. Loci corresponding to BLB resistance were detected using >142,000 SNPs. We identified 147 genes according to employed significance thresholds across chromosomes 1–6, 8, 9 and 11. Moreover, 127 of identified genes are located on chromosomal regions outside estimated Linkage Disequilibrium influences of known resistance genes, potentially indicating novel BLB resistance markers. However, significantly associated SNPs only occurred across a maximum of six Xoo isolates indicating that the development of broad-spectrum Xoo strain varieties may prove challenging. Analyses indicated a range of gene functions likely underpinning BLB resistance. In accordance with previous studies of accession panels focusing on indica varieties, our germplasm displays large numbers of SNPs associated with resistance. Despite encouraging data suggesting that many loci contribute to resistance, our findings corroborate previous inferences that multi-strain resistant varieties may not be easily realised in breeding programs without resorting to multi-locus strategies.

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“…Gene pyramiding is one of the most effective solutions to diseases and insects resistance breakdown. The pyramided lines stacking multiple BPH or BB resistance genes showed broader spectrum, higher level and more durable resistance than the lines with only one gene (Singh et al 2001;Sharma et al 2004;Perez et al 2008;Hu et al 2012;Huang et al 2012;Hu et al 2013;Suh et al 2013;Win et al 2013). Moreover, new conventional varieties and hybrid combinations harboring several resistant traits could be developed by pyramiding multiple resistance genes for different traits.…”

Section: Introductionmentioning

confidence: 99%

Improvement of bacterial blight and brown planthopper resistance in an elite restorer line Huazhan of Oryza

Xiao

et al. 2016

Field Crops Research

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：Development of resistant varieties is one of the most economical and effective strategies to prevent rice from bacterial blight disease (BB) and brown planthopper (BPH), the two main pests jeopardizing rice production. The hybrid rice Tianyouhuazhan (TianfengA/Huazhan) has been widely used in rice production in China, but this hybrid is susceptible to BB and BPH. In this study, one BB resistance gene (Xa23) and two BPH resistance genes (Bph14 and Bph15) were simultaneously introgressed into the restorer line Huazhan to improve the BB and BPH resistance of Tianyouhuazhan using marker-assisted backcrossing (MABC) strategy coupled with phenotypic selection. The results of identification of BB and BPH resistance revealed that almost all of the improved restorer lines and their derived hybrids showed high resistance (HR) or resistance (R) to BB and BPH. Almost all of the agronomic traits of improved restorer lines and their hybrids were similar to those of their respective original versions in field trial, except the 1000-grain weight of improved hybrids showed an extremely significant increase than that of Tianyouhuazhan. Plot yields test in 2013 and 2014 suggested that two improved hybrid combinations named as TianfengA/R43-06 and TianfengA/R43-07 had higher grain

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Marker assisted introgression of multiple genes for bacterial blight resistance into aromatic Myanmar rice MK-75

Cited by 22 publications

References 47 publications

Exploring of bacterial blight resistance in landraces, mining of resistant gene(s) and genetic divergence of resistant germplasm through molecular markers and quantitative traits

Exploring of bacterial blight resistance in landraces, mining of resistant gene(s) and genetic divergence of resistant germplasm through molecular markers and quantitative traits

Identification of Bacterial Blight Resistance Loci in Rice (Oryza sativa L.) against Diverse Xoo Thai Strains by Genome-Wide Association Study

Improvement of bacterial blight and brown planthopper resistance in an elite restorer line Huazhan of Oryza

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