Marker-assisted selection of &lt;i&gt;qMrdd8&lt;/i&gt; to improve maize resistance to rough dwarf disease

Xu, Zhennan; Hua, Jinge; Wang, Feifei; Cheng, Zixiang; Meng, Qingchang; Chen, Yanping; Han, Xiaohua; Tie, Shuanggui; Liu, Changlin; Li, Xinhai; Wang, Zhenhua; Weng, Jianfeng

doi:10.1270/jsbbs.19110

Cited by 31 publications

(10 citation statements)

References 40 publications

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“…MRDD is caused by viruses in the Fijivirus genus in the Reoviridae family (Zhang et al 2001 ; Liu et al 2020a ). In China, outbreaks of MRDD mainly occur in the Huang-Huai-Hai plain (Chen et al 2015 ; Xu et al 2020 ). Yield losses caused by MRDD range from 20 to 30% to as high as 100% in severely infected fields (Xu et al 2020 ).…”

Section: Functional Genomics Of Disease Resistance In Maizementioning

confidence: 99%

Genetic dissection of maize disease resistance and its applications in molecular breeding

Zhu

Tong

et al. 2021

Mol Breeding

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Disease resistance is essential for reliable maize production. In a long-term tug-of-war between maize and its pathogenic microbes, naturally occurring resistance genes gradually accumulate and play a key role in protecting maize from various destructive diseases. Recently, significant progress has been made in deciphering the genetic basis of disease resistance in maize. Enhancing disease resistance can now be explored at the molecular level, from marker-assisted selection to genomic selection, transgenesis technique, and genome editing. In view of the continuing accumulation of cloned resistance genes and in-depth understanding of their resistance mechanisms, coupled with rapid progress of biotechnology, it is expected that the large-scale commercial application of molecular breeding of resistant maize varieties will soon become a reality.

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Section: Functional Genomics Of Disease Resistance In Maizementioning

confidence: 99%

Genetic dissection of maize disease resistance and its applications in molecular breeding

Zhu

Tong

et al. 2021

Mol Breeding

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“…The QTL qMrdd8 from the donor parent X178 was introgressed into seven elite inbred lines from three maize heterotic groups using multi-generation backcrossing and MAS. The seven converted inbred lines and five converted hybrids exhibited enhanced resistance to MRDD across different environments, while other agronomic traits were not affected under non-pathogenic stress conditions [16]. A wheat line carrying the gene for resistance to powdery mildew, Pm21, as donor parent was backcrossed to three spring wheat varieties by combining evaluation for disease resistance with selection using molecular markers.…”

Section: Application Of Qtl For Resistance To Mrdd In Maize Breedingmentioning

confidence: 99%

Fine-mapping the recently discovered QTL qMrdd2 that confers resistance to maize rough dwarf disease

Wang²,

Zhou

et al. 2020

Preprint

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Background: Maize rough dwarf disease (MRDD) is a disease caused by a virus that seriously affects maize yield and quality worldwide. Rice black streaked dwarf virus (RBSDV) in the Fijivirus genus in the Reoviridae family causes MRDD in maize. Typical MRDD symptoms of include severe dwarfing of plants, shortening of internodes. MRDD resistance is a complex trait that is quantitatively inherited and is controlled by several quantitative trait loci (QTL). MRDD is most efficiently controlled by the cultivation of disease-resistant corn hybrids. Results: Disease resistance in the MRDD-resistant Qi319 and -susceptible Ye478 parental inbred lines and the 314 recombinant inbred lines (RILs) that were derived from a cross between them was evaluated across three environments. A stable resistance QTL, qMrdd2 , which explained 8.64 to 11.02% of the total phenotypic variance in MRDD resistance, was identified repeatedly and was mapped using BLUP values to a 0.55-Mb region between the markers MK807 and MK811 on chromosome 2. We validated the effect of qMrdd2 using a chromosome segment substitution line population that were derived from a cross between maize inbred Qi319 as the resistance donor and Ye478 as the recipient. The disease-severity index (DSI) of CSSL haplotype II harboring qMrdd2 was significantly lower than the DSI of susceptible parent Ye478 ( P < 0.05). Mapping results using CSSLs were consistent with localization interval determined using RILs. The qMrdd2 locus acted with an additive effect but no significant dominant gene action in conferring MRDD resistance. We fine-mapped qMrdd2 locus into a 315-kb region flanked by the markers RD81 and RD87 by testing recombinant-derived progeny using selfed backcrossed families. Conclusions: qMrdd2 is a recently discovered QTL from Qi319 for resistance to MRDD with an additive effect but no significant dominant gene action for MRDD resistance. qMrdd2 was fine-mapped to a 315-kb interval on maize chromosome 2. Introgression of the MRDD resistance allele at the qMrdd2 locus of CSSL haplotype 2 using linked markers umc1824 and bnlg125 will be useful for maize breeding to reduce yield losses caused by MRDD. Keywords: Maize, Maize rough dwarf disease, QTL, Fine-mapping, RIL；CSSL.

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“…Subsequently, the favorable alleles are transferred from the donor lines to the recipient lines for the improvement of the target trait. Several maize breeding programs have reported improved selection efficiency upon implementation of MAS (Nair et al 2015;Xu et al 2020;Prasanna et al 2020aPrasanna et al , 2021. At CIMMYT, MAS is being routinely deployed to enrich the favorable alleles of large effect QTL for maize lethal necrosis, maize streak virus (MSV), and provitamin A content in tropical maize breeding populations (Prasanna et al 2020a(Prasanna et al , b, 2021.…”

Section: Introductionmentioning

confidence: 99%

Identification and fine mapping of a major QTL (qRtsc8-1) conferring resistance to maize tar spot complex and validation of production markers in breeding lines

Ren

Huestis

et al. 2022

Theor Appl Genet

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Key message A major QTL of qRtsc8-1 conferring TSC resistance was identified and fine mapped to a 721 kb region on chromosome 8 at 81 Mb, and production markers were validated in breeding lines. Abstract Tar spot complex (TSC) is a major foliar disease of maize in many Central and Latin American countries and leads to severe yield loss. To dissect the genetic architecture of TSC resistance, a genome-wide association study (GWAS) panel and a bi-parental doubled haploid population were used for GWAS and selective genotyping analysis, respectively. A total of 115 SNPs in bin 8.03 were detected by GWAS and three QTL in bins 6.05, 6.07, and 8.03 were detected by selective genotyping. The major QTL qRtsc8-1 located in bin 8.03 was detected by both analyses, and it explained 14.97% of the phenotypic variance. To fine map qRtsc8-1, the recombinant-derived progeny test was implemented. Recombinations in each generation were backcrossed, and the backcross progenies were genotyped with Kompetitive Allele Specific PCR (KASP) markers and phenotyped for TSC resistance individually. The significant tests for comparing the TSC resistance between the two classes of progenies with and without resistant alleles were used for fine mapping. In BC5 generation, qRtsc8-1 was fine mapped in an interval of ~ 721 kb flanked by markers of KASP81160138 and KASP81881276. In this interval, the candidate genes GRMZM2G063511 and GRMZM2G073884 were identified, which encode an integral membrane protein-like and a leucine-rich repeat receptor-like protein kinase, respectively. Both genes are involved in maize disease resistance responses. Two production markers KASP81160138 and KASP81160155 were verified in 471 breeding lines. This study provides valuable information for cloning the resistance gene, and it will also facilitate the routine implementation of marker-assisted selection in the breeding pipeline for improving TSC resistance.

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Marker-assisted selection of <i>qMrdd8</i> to improve maize resistance to rough dwarf disease

Cited by 31 publications

References 40 publications

Genetic dissection of maize disease resistance and its applications in molecular breeding

Genetic dissection of maize disease resistance and its applications in molecular breeding

Fine-mapping the recently discovered QTL qMrdd2 that confers resistance to maize rough dwarf disease

Identification and fine mapping of a major QTL (qRtsc8-1) conferring resistance to maize tar spot complex and validation of production markers in breeding lines

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