Markers for selection of three alleles of ClSUN25-26-27a (Cla011257) associated with fruit shape in watermelon

Legendre, Reeve; Kuzy, Jesse Daniel; McGregor, Cecilia

doi:10.1007/s11032-020-1104-2

Cited by 17 publications

(23 citation statements)

References 28 publications

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“…Interestingly, we detected a major-effect QTL (PVE = 35.5-50.1%) for SL, SW, and 20SWT (20 seed weight) on chromosome 2 by two watermelon inbred lines with medium size seeds, and we detected none QTL on chromosome 6. Indeed, the major-effect QTL was probably the same one detected in multiple previous studies controlling seed size variation in watermelon [1,17,76]. So the QTL on chromosome 2 may control medium size seed variation in watermelon.…”

Section: Qtl For Fruit Size and Shape In Watermelonsupporting

confidence: 63%

“…found that the round fruit shape is due to a 159-bp deletion in the coding region of Cla011257 [74]. This nding was con rmed by Maragal et al (2019) and Legendre et al (2020) who reported also a novel allele at this locus [75][76].…”

Section: Qtl For Fruit Size and Shape In Watermelonmentioning

confidence: 68%

“…Among them, qsl5.1, qsl5.2, qsl7.1, qsw3.1, qsw10.1, q20swt10.1 were not identi ed in previous studies. Especially, the QTL on chromosome 6, was identi ed in most studies with F 2 and RILs populations derived from different seed size materials, suggesting that this QTL may play the most important role in seed size/weight determination [17,54,55,76]. Interestingly, we detected a major-effect QTL (PVE = 35.5-50.1%) for SL, SW, and 20SWT (20 seed weight) on chromosome 2 by two watermelon inbred lines with medium size seeds, and we detected none QTL on chromosome 6.…”

Section: Qtl For Fruit Size and Shape In Watermelonmentioning

confidence: 99%

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QTL Mapping of Fruit and Seed-related Traits in Watermelon Using Genotyping-by-Sequencing-based High-density Linkage Mapping

Gao

Liang

Liu³

et al. 2020

Preprint

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BackgroundWatermelon is an important vegetable crop with dual use of both fruit and seeds. Understanding the genetic basis of fruit quality and seed size-related traits is important for efficient marker-assisted breeding in watermelon. Linkage mapping in watermelon segregating populations using genotyping-by-sequencing (GBS) provides insights into genetic control of fruit- and seed-related traits and genome collinearity in commercial watermelon cultivars. ResultsIn the present study, we conducted QTL mapping of 12 horticulturally important traits on external and internal fruit quality and seed size/weight using segregating populations derived a cross between two commercial varieties. A high-density genetic map was developed with GBS which contained more than 6,000 SNP loci in 1,004 bins with a total map length of 1261.1 cM and average marker interval of 1.26 cM or 329 kb. Phenotypic data of fruit rind color (RC), rind stripe pattern (RSP), flesh color (FFC), fruit diameter (FD), fruit length (FL), fruit shape index (FSI), fruit weight (FW), Brix content central (BCC), Brix content edge (BCE), seed length (SL), width (SW), and weight (20SWT) were collected from two locations in two years. QTL analysis identified 47 QTL for the 12 traits, of which 24 had moderate- or major-effects, and 34 were novel QTL not identified in previous studies. The QTL for RSP were identified overlapped with previous reports, and mapped the QTL to a small interval on chromosome 6. From the detected novel QTL, we identify FD (qfd2.1), FL (qfl2.1) co-located with FSI (qfsi2.1) QTL on chromosome 2, and the minor QTL qfw3.2 co-located with previously reported fruit shape QTL (qfd3.1, qfl3.1, qfsi3.1), and SW (qsw10.1) co-located with 20SWT QTL (q20swt10.1) on chromosome 10, and 5 minor QTL (qbcc2.1, qbcc5.1, qbce2.1, qbce2.2, qbce5.1) were found to be likely new locus for Brix content.ConclusionWe conducted GBS consisting of 120 F2 individuals and developed a high-density linkage map with more than 6,000 SNP loci in 1004 bins in watermelon. We identified 47 QTL for 12 fruit and seed related traits including 34 novel QTL. Our work expands the molecular breeding toolbox for watermelon to improve the yield and fruit quality.

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Section: Qtl For Fruit Size and Shape In Watermelonsupporting

confidence: 63%

Section: Qtl For Fruit Size and Shape In Watermelonmentioning

confidence: 68%

Section: Qtl For Fruit Size and Shape In Watermelonmentioning

confidence: 99%

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QTL Mapping of Fruit and Seed-related Traits in Watermelon Using Genotyping-by-Sequencing-based High-density Linkage Mapping

Gao

Liang

Liu³

et al. 2020

Preprint

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“…Functional genes conditioning qualitative and quantitative phenotypic traits in watermelon have been identified through comparative genetic analysis [11][12][13][14][15][16][17]. Qualitative phenotypic traits in watermelon are conditioned by major genes [13,14,[18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Retrospective Genetic Analysis of Qualitative and Quantitative Traits in Sweet Watermelon (Citrullus lanatus var. lanatus): A Review

et al. 2022

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Understanding the genetic basis of a crop’s qualitative and quantitative traits is vital to designing market preferred varieties. The aim of this review is to present a retrospective genetic analysis of qualitative and quantitative phenotypic traits in sweet watermelon as a guide for trait integration and the development of novel varieties with yield potential and desirable horticultural attributes. The first section outlines genes conditioning the inheritance of plant architecture (e.g., leaf attributes and plant architecture), floral characters (flowering rate, sex expression, and male sterility), fruit traits (shape, colour, rind colour and stripe patterns and flesh colour) and seed morphology (seed length, width, size and coat colour). In the second section, developments in molecular markers and quantitative trait loci (QTL) to aid marker-assisted breeding are discussed. Further, the review highlights the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) gene-editing technology and its scope in gene manipulations and new variety development. The information presented in this review is useful for optimised and demand-led breeding to develop new varieties to serve growers, consumers and the sweet watermelon industry.

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“…Subsequently, Dou et al (2018) located fsi3.1 in the 26.817-26.863 Mb region on chromosome 3, and determined that a 159-bp deletion in Cla011257 was responsible for fruit elongation; a total of 105 watermelon lines were tested, and all lines with long and thin fruits had this deletion. The Cla017257 allele also causes other variations in fruit shape (Legendre et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Fine mapping and identification of the candidate gene BFS for fruit shape in wax gourd (Benincasa hispida)

Cheng

Liu

et al. 2021

Theor Appl Genet

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Fruit shape is an important agronomic trait in wax gourds [Benincasa hispida (Thunb) Cogn.]. However, the candidate genes for this important trait, and their genetic mechanisms, remain unknown. In this study, we identi ed a candidate gene for fruit shape in wax gourds using a next-generation sequencing-based bulked segregant analysis in F 2 populations derived from a cross between GX-71 (long cylindrical fruit, FSI = 4.56) and MY-1 (round fruit, FSI = 1.06) genotypes. According to bulked segregant analysis, the candidate gene is located in the 17.18 Mb region on chromosome 2, and the kompetitive allele speci c polymerase chain reaction (KASP) marker was used to reduce it to the 19.6 Kb region. There is only one gene in the corresponding region of the reference genome, Bch02G016830 (designated BFS). We sequenced BFS in six wax gourd varieties with different fruit shapes. Sequence analysis showed that there were two non-synonymous mutations in the spherical wax gourd and one non-synonymous mutation in the cylindrical wax gourd. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that the expression of BFS in round fruits was signi cantly higher than in long cylindrical fruits at the ovary formation stage. Therefore, BFS is a candidate gene for determination of the fruit shape of wax gourds. The predicted protein encoded by the BFS gene belongs to the IQD protein family, which have the structural characteristics of scaffold proteins and coordinate Ca 2+ CaM signaling from the membrane to the nucleus. The BFS gene can assist with the breeding of new varieties that possess ideal fruit shapes. Key MessageNon-synonymous mutations in the BFS gene, which encodes the IQD protein, are responsible for the shape of wax gourd fruits.

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Markers for selection of three alleles of ClSUN25-26-27a (Cla011257) associated with fruit shape in watermelon

Cited by 17 publications

References 28 publications

QTL Mapping of Fruit and Seed-related Traits in Watermelon Using Genotyping-by-Sequencing-based High-density Linkage Mapping

QTL Mapping of Fruit and Seed-related Traits in Watermelon Using Genotyping-by-Sequencing-based High-density Linkage Mapping

Retrospective Genetic Analysis of Qualitative and Quantitative Traits in Sweet Watermelon (Citrullus lanatus var. lanatus): A Review

Fine mapping and identification of the candidate gene BFS for fruit shape in wax gourd (Benincasa hispida)

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