MASDET—A fast and user-friendly multiplatform software for mass determination by dark-field electron microscopy

Krzyžánek, Vladislav; Müller, Shirley A.; Engel, Andréas; Reichelt, Rudolf

doi:10.1016/j.jsb.2008.10.006

Cited by 29 publications

(27 citation statements)

References 41 publications

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“…Mass analysis was carried out with the MASDET program package (Krzyzanek et al, 2009). Particles having a size between ~100-200 Å were selected in optimized circular boxes, total mass was calculated and background subtracted.…”

Section: Methodsmentioning

confidence: 99%

Rhodopsin–transducin heteropentamer: Three-dimensional structure and biochemical characterization

Jastrzębska

Ringler

Lodowski

et al. 2011

Journal of Structural Biology

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The process of vision is initiated when the G protein-coupled receptor, rhodopsin (Rho), absorbs a photon and transitions to its activated Rho* form. Rho* binds the heterotrimeric G protein, transducin (Gt) inducing GDP to GTP exchange and Gt dissociation. Using nucleotide depletion and affinity chromatography, we trapped and purified the resulting nucleotide-free Rho*•Gt complex. Quantitative SDS-PAGE suggested a 2:1 molar ratio of Rho* to Gt in the complex and its mass determined by scanning transmission electron microscopy was 221±12 kDa. A 21.6Å structure then was calculated from projections of negatively stained Rho*•Gt complexes. The molecular envelope thus determined accommodated two Rho molecules together with one Gt heterotrimer, corroborating the heteropentameric structure of the Rho*•Gt complex.

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Section: Methodsmentioning

confidence: 99%

Rhodopsin–transducin heteropentamer: Three-dimensional structure and biochemical characterization

Jastrzębska

Ringler

Lodowski

et al. 2011

Journal of Structural Biology

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“…Mass analysis was carried out with the MASDET program package. 89 In short, fibril segments were selected in square boxes and tracked. The total scattering within an integration box, following their length, was then calculated, and the scattering contribution of the supporting carbon film was deducted.…”

Section: Scanning Transmission Electron Microscopymentioning

confidence: 99%

A Structural Model for Apolipoprotein C-II Amyloid Fibrils: Experimental Characterization and Molecular Dynamics Simulations

Teoh

Pham

Todorova

et al. 2011

Journal of Molecular Biology

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“…The recording dose used varied between 290 and 635 electrons/nm 2 . The images were evaluated with the MASDET program package as described previously (Krzyžánek et al, 2009). All mass--per--length (MPL) data were corrected for beam--induced mass--loss.…”

Section: Scanning Transmission Electron Microscopymentioning

confidence: 99%

Connecting μ-fluidics to electron microscopy

Kemmerling

Ziegler

Schweighauser

et al. 2012

Journal of Structural Biology

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A versatile methodology for electron microscopy (EM) grid preparation enabling total content sample analysis is presented. A microfluidic-dialysis conditioning module to desalt or mix samples with negative stain solution is used, combined with a robotic writing table to micro-pattern the EM grids. The method allows heterogeneous samples of minute volumes to be processed at physiological pH for structure and mass analysis, and allows the preparation characteristics to be finely tuned.

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MASDET—A fast and user-friendly multiplatform software for mass determination by dark-field electron microscopy

Cited by 29 publications

References 41 publications

Rhodopsin–transducin heteropentamer: Three-dimensional structure and biochemical characterization

Rhodopsin–transducin heteropentamer: Three-dimensional structure and biochemical characterization

A Structural Model for Apolipoprotein C-II Amyloid Fibrils: Experimental Characterization and Molecular Dynamics Simulations

Connecting μ-fluidics to electron microscopy

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