Maskless Arrayed Nanofiber Mats by Bipolar Pyroelectrospinning

Rega, Romina; Gennari, Oriella; Mecozzi, Laura; Pagliarulo, Vito; Bramanti, Alessia; Ferraro, Pietro; Grilli, Simonetta

doi:10.1021/acsami.8b12513

Cited by 19 publications

(17 citation statements)

References 52 publications

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“…For each collagen solution we proceeded in the following way: We deposited manually 0.2 μL of the collagen solution onto the tip of the base support by using a Hamilton syringe. The use of the tip as support allows us to expose a thinner liquid meniscus to the electric field, thus guaranteeing high repeatable droplet ejection [ 44 , 45 ]. We call this drop the “mother drop” and Figure 1 d shows the schematic side view, where the dashed line corresponds to the liquid–air interface.…”

Section: Resultsmentioning

confidence: 99%

Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation

Rega

Mugnano

Oleandro

et al. 2020

Sensors

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The demand for sensors capable of measuring low-abundant collagen in human fluids has highly increased in recent years. Indeed, collagen is expected to be a biomarker for chronic diseases and could monitor their progression. Here we show detection of highly diluted samples of collagen at picogram level thanks to an innovative pyro-electrohydrodynamic jet (p-jet) system. Through the intense electric fields generated by the pyroelectric effect in a ferroelectric crystal, the collagen solution was concentrated on a small area of a slide that was appropriately functionalized to bind proteins. The collagen molecules were labeled by an appropriate fluorophore to show how the number of tiny droplets influences the limit of detection of the technique. The results show that the p-jet is extremely promising for overcoming the current detection limits of collagen-based products in human fluids, performing 10 times better than the enzyme-linked immunosorbent assay (ELISA) and thus paving the way for the early diagnosis of related chronic diseases.

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Section: Resultsmentioning

confidence: 99%

Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation

Rega

Mugnano

Oleandro

et al. 2020

Sensors

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“…The pyroelectric effect generates an array of surface charges with opposite sign, following the pattern of the reversed domains. Recently, we demonstrated for the first time the possibility of using the pyroelectric effect for a wide variety of applications ranging from biological to soft matter manipulation application (Ferraro et al, 2010; Mecozzi et al, 2016, 2017; Rega et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

Pyro-Electrification of Freestanding Polymer Sheets: A New Tool for Cation-Free Manipulation of Cell Adhesion in vitro

et al. 2019

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Localized electric fields have become, in recent years, a source of inspiration to researchers and laboratories thanks to a huge amount of applications derived from it, including positioning of microparticles as building blocks for electrical, optical, and magnetic devices. The possibility of producing polymeric materials with surface charge thus opens new perspectives for applications where process simplicity and cost-effectiveness of flexible electronics are of fundamental importance. In particular, the influence of surface charges is widely studied and is a critical issue especially when new materials and functional technologies are introduced. Here, we report a voltage-free pyro-electrification (PE) process able to induce a permanent dipole orientation into polymer sheets under both mono- and bipolar distribution. The technique makes use of the pyroelectric effect for generating electric potentials on the order of kilovolts by an easy-to-accomplish thermal treatment of ferroelectric lithium niobate (LN) crystals. The PE allows us to avoid the expensive and time-consuming fabrication of high-power electrical circuits, as occurs in traditional generator-based techniques. Since the technique is fully compatible with spin-coating-based procedures, the pyro-electrified polymer sheets are easily peeled off the surface of the LN crystal after PE completion, thus providing highly stable and freestanding charged sheets. We show the reliability of the technique for different polymers and for different applications ranging from live cell patterning to biofilm formation tests for bacteria linked to food-processing environments.

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“…Recently, Grilli et al invented bipolar pyroelectrospinning, which is capable of generating ordered arrays of nanofibers by exploiting periodically poled lithium niobate (PPLN) as a substrate [ 81 ]. First, PPLN crystal was patterned in a hexagonal shape, where ferroelectric domains are formed with reversed polarization, achieved by a standard electric field poling onto a template sample defined with photolithography.…”

Section: Structural and Architectural Regulationsmentioning

confidence: 99%

Recent Advances on Nanofiber Fabrications: Unconventional State-of-the-Art Spinning Techniques

2020

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In this review, we describe recent relevant advances in the fabrication of polymeric nanofibers to address challenges in conventional approaches such as electrospinning, namely low throughput and productivity with low size uniformity, assembly with a regulated structure and even architecture, and location with desired alignments and orientations. The efforts discussed have mainly been devoted to realize novel apparatus designed to resolve individual issues that have arisen, i.e., eliminating ejection tips of spinnerets in a simple electrospinning system by effective control of an applied electric field and by using mechanical force, introducing a uniquely designed spinning apparatus including a solution ejection system and a collection system, and employing particular processes using a ferroelectric material and reactive precursors for atomic layer deposition. The impact of these advances to ultimately attain a fabrication technique to solve all the issues simultaneously is highlighted with regard to manufacturing high-quality nanofibers with high- throughput and eventually, practically implementing the nanofibers in cutting-edge applications on an industrial scale.

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Maskless Arrayed Nanofiber Mats by Bipolar Pyroelectrospinning

Cited by 19 publications

References 52 publications

Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation

Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation

Pyro-Electrification of Freestanding Polymer Sheets: A New Tool for Cation-Free Manipulation of Cell Adhesion in vitro

Recent Advances on Nanofiber Fabrications: Unconventional State-of-the-Art Spinning Techniques

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