Maspin and MCM2 immunoprofiling in salivary gland carcinomas

Abstract

BackgroundThe pathogenesis of salivary gland carcinomas is very complex and prognostic markers are difficult to find in these carcinomas of which the different subtypes have varying malignant potential. The study was conducted to examine the cellular distribution of maspin and MCM2 in salivary gland carcinomas and their value to predict lymph node metastasis.Materials and methodsFifty three paraffin blocks of different lesions (15 muco-epidermoid carcinoma, 14 adenoid cystic carcinoma, 3 epi-myoepithelial carcinoma, 5 salivary duct carcinoma, 5 malignant pleomorphic adenoma, 6 polymorphous low grade adenocarcinoma and 5 acinic cell carcinoma) were prepared for immunohistochemical staining with maspin and MCM2 antibodies. ANOVA and Pearson correlation tests were used for the statistical analysis of the results.ResultsAll salivary gland carcinomas express maspin and MCM2 with variable cellular localization. There was a significant difference in the expression of each antibody between mucoepidermoid carcinoma, adenoid cystic carcinoma and polymorphous low grade adenocarcinoma. No association was found between examined markers and lymph node metastasis.ConclusionsSalivary gland carcinomas express maspin and MCM2 with variable levels and cellular localization, consisting important markers of biological behavior in these tumors. The level of MCM2 expression can be used in the differential diagnosis of adenoid cystic carcinoma and polymorphous low grade adenocarcinoma. Further study with large sample size is recommended to assess their value in prediction of lymph node metastasis.

Prognostic Value of P63, Maspin, and MMP-2 Expression in Salivary Gland Adenoid Cystic Carcinoma

“…In accordance with previous studies (20,21), the results of this study showed inverse significant association between maspin expression and histologic grade. No significant correlation was found between maspin expression and lymph node metastasis, which was similar to Ghazy et al's study (22), but in contrast to the findings of Schwartz et al (23). This discrepancy may be attributed to the method of expression evaluation and the number of samples .Of note, we encountered limitation in sample collection due to incomplete data in patients' record and lack of paraffin blocks.…”

Adenoid cystic carcinoma of the head and neck – An update

“…Cho et al [46] suggested that high PCNA (proliferating cell nuclear antigen) expression is significantly associated with shorter disease-free and overall survival of patients with AdCCs. Minichromosome maintenance (MCM) protein expression has been found to be a novel marker for proliferating tumor cells and is diagnostically useful for the differential diagnosis of benign and malignant salivary gland tumors [47]. The expression of the argyrophylic nucleolar organizer region (AgNOR) associated proteins were also an independent prognostic factor in these tumors [48].…”

Expression of Human Tissue Kallikreins (KLKs) in Polymorphous Low Grade Adenocarcinoma (PLGA)

“…Salivary gland tumours, including PLGA and ACC, stained positively for both maspin and MCM2 to a variably degree, but ACC showed a higher proliferation profile versus PLGA. There was no significant correlation between maspin and MCM2 expression and regional metastatic disease [48]. …”

“…Maspin is a mammary serine protease inhibitor, that functions to prevent apoptosis and decrease cell invasion, and may have a role in preventing angiogenesis [48]. MCM2's are mini chromosome maintenance proteins, which are paramount for DNA replication and therefore, have use as markers for cell proliferation [48].…”

“…MCM2's are mini chromosome maintenance proteins, which are paramount for DNA replication and therefore, have use as markers for cell proliferation [48]. In 2011, Ghazy et al studied the expression of maspin and MCM2 in salivary gland tumours, and specifically wanted to determine if their expression correlated to lymph node metastasis [48]. Salivary gland tumours, including PLGA and ACC, stained positively for both maspin and MCM2 to a variably degree, but ACC showed a higher proliferation profile versus PLGA.…”