Mass and Charge Measurements on Heavy Ions

Sugai, Toshiki

doi:10.5702/massspectrometry.s0074

Cited by 7 publications

(4 citation statements)

References 89 publications

(260 reference statements)

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“…For more than 50 years there has been an enduring effort to increase the size of the highest molecular weight ions that can be analyzed by mass spectrometry (MS). The development of electrospray and MALDI (matrix-assisted laser desorption/ionization) in the 1980s , opened the door and facilitated studies of large biomolecular complexes. − High molecular weight ions generated by electrospray are highly charged, and in conventional MS, where only the m / z is measured, the charge must be deduced from the charge state envelope in the m / z spectrum. The upper mass limit for conventional MS is realistically less than a megadalton for a typical heterogeneous sample.…”

mentioning

confidence: 99%

Higher Resolution Charge Detection Mass Spectrometry

et al. 2020

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Charge detection mass spectrometry is a single particle technique where the masses of individual ions are determined from simultaneous measurements of each ion’s m/z ratio and charge. The ions pass through a conducting cylinder, and the charge induced on the cylinder is detected. The cylinder is usually placed inside an electrostatic linear ion trap so that the ions oscillate back and forth through the cylinder. The resulting time domain signal is analyzed by fast Fourier transformation; the oscillation frequency yields the m/z, and the charge is determined from the magnitudes. The mass resolving power depends on the uncertainties in both quantities. In previous work, the mass resolving power was modest, around 30–40. In this work we report around an order of magnitude improvement. The improvement was achieved by coupling high-accuracy charge measurements (obtained with dynamic calibration) with higher resolution m/z measurements. The performance was benchmarked by monitoring the assembly of the hepatitis B virus (HBV) capsid. The HBV capsid assembly reaction can result in a heterogeneous mixture of intermediates extending from the capsid protein dimer to the icosahedral T = 4 capsid with 120 dimers. Intermediates of all possible sizes were resolved, as well as some overgrown species. Despite the improved mass resolving power, the measured peak widths are still dominated by instrumental resolution. Heterogeneity makes only a small contribution. Resonances were observed in some of the m/z spectra. They result from ions with different masses and charges having similar m/z values. Analogous resonances are expected whenever the sample is a heterogeneous mixture assembled from a common building block.

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confidence: 99%

Higher Resolution Charge Detection Mass Spectrometry

et al. 2020

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“…[ [230][231][232][233][234][235] Aleixandre-Tudo and Toit [220] wrote a chapter about the quantification of phenols during the winemaking process by UV-Vis spectroscopy, in which they refer that spectrophotometric methods are commonly used to analyze the content of total phenols, anthocyanins, tannins, and color, referring to several protocols that could be applied to wine and wine byproduct samples.…”

Section: Mass Spectrometrymentioning

confidence: 99%

Beyond the Bottle: Exploring Health-Promoting Compounds in Wine and Wine-Related Products—Extraction, Detection, Quantification, Aroma Properties, and Terroir Effects

Marques,

Dinis,

Santos

et al. 2023

Foods

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Health-promoting compounds in wine and wine-related products are important due to their potential benefits to human health. Through an extensive literature review, this study explores the presence of these compounds in wine and wine-related products, examining their relationship with terroir and their impact on the aromatic and flavor properties that are perceived orally: sunlight exposure, rainfall patterns, and soil composition impact grapevines’ synthesis and accumulation of health-promoting compounds. Enzymes, pH, and the oral microbiome are crucial in sensory evaluation and perception of health promotion. Moreover, their analysis of health-promoting compounds in wine and wine-related products relies on considerations such as the specific target compound, selectivity, sensitivity, and the complexity of the matrix.

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“…The generation of micro/nanodroplets through ESI or nano-ESI serves as a fundamental purpose in producing biological macroions comprising nanometer- and micrometer-sized bioparticles, such as viruses − and bacteria, , in the gas phase. These macroions are typically detected by electrostatic ion traps ,− and orbitraps, , equipped with charge detectors, enabling the determination of their mass through charge numbers ( Z ) and mass-to-charge ( m / z ) ratios. − However, charged solvent microdroplets produced during ESI caused potential interference with bioparticle mass measurements.…”

Section: Introductionmentioning

confidence: 99%

Real-Time Monitoring of the Evaporation and Fission of Electrospray-Ionized Polystyrene Beads and Bacterial Pellets at Elevated Temperatures

Liang,

Cua Estayan,

Hsieh

et al. 2024

Anal. Chem.

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This study uses real-time monitoring, at microsecond time scales, with a charge-sensing particle detector to investigate the evaporation and fission processes of methanol/ micrometer-sized polystyrene beads (PS beads) droplets and bacterial particles droplets generated via electrospray ionization (ESI) under elevated temperatures. By incrementally raising capillary temperatures, the solvent, such as methanol on 0.75 μm PS beads, experiences partial evaporation. Further temperature increase induces fission, and methanol molecules continue to evaporate until PS ions are detected after this range. Similar partial evaporation is observed on 3 μm PS beads. However, the shorter period of the fission temperature range is necessary compared to 0.75 μm PS beads. For the spherical-shaped bacterium, Staphylococcus aureus, the desolvation process shows a similar fission period as compared to 0.75 μm PS beads. Comparably, the rodshaped bacteria, Escherichia coli EC11303, and E. coli strain W have shorter fission periods than S. aureus. This research provides insights into the evaporation and fission mechanisms of ESI droplets containing different sizes and shapes of micrometer-sized particles, contributing to a better understanding of gaseous macroion formation.

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Mass and Charge Measurements on Heavy Ions

Cited by 7 publications

References 89 publications

Higher Resolution Charge Detection Mass Spectrometry

Higher Resolution Charge Detection Mass Spectrometry

Beyond the Bottle: Exploring Health-Promoting Compounds in Wine and Wine-Related Products—Extraction, Detection, Quantification, Aroma Properties, and Terroir Effects

Real-Time Monitoring of the Evaporation and Fission of Electrospray-Ionized Polystyrene Beads and Bacterial Pellets at Elevated Temperatures

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