Mass-measurement-type optical particle counting method for determination of number concentration of liquid-borne particles

Kuruma, Yuki; Sakaguchi, Takayuki

doi:10.1016/j.apt.2019.12.003

Cited by 6 publications

(20 citation statements)

References 9 publications

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“…In addition, its measurable size range was configured differently compared to that of standard KS-28B model (200 nm-2 μm). In our previous study, agreement between the measurement results obtained via the two OPCs in the overlapping size range (i.e., at 2 μm) was confirmed [19]. In this study, the KS-28B OPC was used for the 600 nm suspensions, while the KS-65 OPC was used for the 10 μm suspensions.…”

Section: Mass-measurement-type Optical Particle Counting Methods Meas...supporting

confidence: 79%

“…Figure 1 shows a schematic of the M-OPC measurement method, while a full description of the method is provided in a previous report [19]. Thus, we only briefly describe the principle and measurement procedure of the M-OPC method in this paper.…”

Section: Mass-measurement-type Optical Particle Counting Methods Meas...mentioning

confidence: 99%

“…An electronic balance (XP504DR, Mettler Toledo, USA) was used for mass measurements in the dilution procedure. The details of the preparation and dispersion procedures were described in a previous paper [19], with the preparation of the 1k suspension from the 1M suspensions replicated to supply five bottles for each diameter. It should be noted that the PSL particles used in this study were confirmed to be sufficiently stable during our experiment even though these dilution factors were more than 1000 without adding any surfactants.…”

Section: Target Suspensions and Preparation Proceduresmentioning

confidence: 99%

“…In 2018, the NMIJ developed a new accurate measurement method known as the mass-measurement-type optical particle counting (M-OPC) method to serve as the primary standard for the number concentration of liquid-borne particles [19]. This method replaced the previous flow cytometer method (T-FCM) and covers an extended diameter range of 600 nm-10 μm and the same concentration range (i.e., 500 particles g −1 -2000 000 particles g −1 ).…”

Section: Introductionmentioning

confidence: 99%

“…This method replaced the previous flow cytometer method (T-FCM) and covers an extended diameter range of 600 nm-10 μm and the same concentration range (i.e., 500 particles g −1 -2000 000 particles g −1 ). The details of the measurement procedures were provided in a previous report, which demonstrated the validity of the new method using a monodisperse 2 μm PSL suspension [19].…”

Section: Introductionmentioning

confidence: 99%

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Primary standard for the number concentration of liquid-borne particles: extension of the diameter range from the micrometer to the sub-micrometer level and reduction of the measurement uncertainty

Kuruma¹,

Sakaguchi²

2021

Metrologia

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The primary measurement standard for the number concentration of liquid-borne particles adopted by the National Metrology Institute of Japan was upgraded to cover particle diameters in the range of 600 nm–10 µm. The main aim was to report the extension of the lower limit of the measurement range in particle diameters from 2 µm to 600 nm, and the reduction of the measurement uncertainty inherent in the previous method using the total-counting-type flow cytometric technique. To extend the measurement range, we adopted a mass-measurement-type optical particle counting method, which consisted of a pair of optical particle counters with a pulse height analyzer, an electronic balance, and a syringe sampler. The relative expanded uncertainties with a coverage factor of k = 2 for the 600 nm and 10 µm suspension measurements were 4.3% and 3.5%, respectively. The validity of the results was confirmed in both cases via comparison with the results obtained using an independent microscopic counting method. With the change in the measurement method, a reduction of the uncertainty was accomplished: e.g., from a 4.4% to a 3.5% relative expanded uncertainty (k = 2) for a 10 µm polystyrene latex particle suspension.

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Section: Mass-measurement-type Optical Particle Counting Methods Meas...supporting

confidence: 79%

Section: Mass-measurement-type Optical Particle Counting Methods Meas...mentioning

confidence: 99%

Section: Target Suspensions and Preparation Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Primary standard for the number concentration of liquid-borne particles: extension of the diameter range from the micrometer to the sub-micrometer level and reduction of the measurement uncertainty

Kuruma¹,

Sakaguchi²

2021

Metrologia

Self Cite

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Use of a sample injection loop for an accurate measurement of particle number concentration by flow cytometry

Shin,

Yang,

Park

et al. 2024

Anal Bioanal Chem

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Flow cytometry plays a pivotal role in biotechnology by providing quantitative measurements for a wide range of applications. Nonetheless, achieving precise particle quantification, particularly without relying on counting beads, remains a challenge. In this study, we introduce a novel exhaustive counting method featuring a sample loop–based injection system that delivers a defined sample volume to a detection system to enhance quantification in flow cytometry. We systematically assess the performance characteristics of this system with micron-sized polystyrene beads, addressing issues related to sample introduction, adsorption, and volume measurement. Results underscore the excellent analytical performance of the proposed method, characterized by high linearity and repeatability. We compare our approach to counting bead–based measurements, and while an approximate bias value was observed, the measured values were found to be similar between the methods, demonstrating its comparability and reliability. This method holds great promise for improving the accuracy and precision of particle quantification in flow cytometry, with implications for various fields including healthcare and environmental monitoring.

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Optimization of Flow Imaging Microscopy Setting Using Spherical Beads with Optical Properties Similar to Those of Biopharmaceuticals

Kurinomaru,

Takeda,

Onaka

et al. 2023

Journal of Pharmaceutical Sciences

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Mass-measurement-type optical particle counting method for determination of number concentration of liquid-borne particles

Cited by 6 publications

References 9 publications

Primary standard for the number concentration of liquid-borne particles: extension of the diameter range from the micrometer to the sub-micrometer level and reduction of the measurement uncertainty

Primary standard for the number concentration of liquid-borne particles: extension of the diameter range from the micrometer to the sub-micrometer level and reduction of the measurement uncertainty

Use of a sample injection loop for an accurate measurement of particle number concentration by flow cytometry

Optimization of Flow Imaging Microscopy Setting Using Spherical Beads with Optical Properties Similar to Those of Biopharmaceuticals

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